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The map label for this gene is yfkN [H]

Identifier: 194735507

GI number: 194735507

Start: 4192377

End: 4193933

Strand: Direct

Name: yfkN [H]

Synonym: SeSA_A4322

Alternate gene names: 194735507

Gene position: 4192377-4193933 (Clockwise)

Preceding gene: 194737573

Following gene: 194734842

Centisome position: 89.03

GC content: 52.09

Gene sequence:

>1557_bases
ATGAAAGTAAAACTGCTTGCTGCCGGTATTTTGTTCACGCTGCCGTTCTGGGCCTGCGCCAAAGATGTCACCATTATTTA
CACCAACGATCTACACGCCCATGTGGAGCCTTATAAAGTGCCGTGGATTGCTGACGGTAAACGCGATATTGGCGGTTGGG
CCAATATCACTACGCTGGTGAAGCAGGAAAAAGCCAAAAATAAGGCGACGTGGTTTTTTGATGCCGGAGATTACTTTACC
GGACCGTATATCAGCAGTCTGACGAAGGGTAAAGCGATTATCGATATTTTGAATACCATGCAGTATGACGCTGCCACTAT
CGGTAATCATGAGTTCGATCATGGTTGGGACAATACATTGTTGCAACTGAGCCGGGCAAAATTCCCTATCGTGCAGGGCA
ATATTTTTTATGAGGACAGCAGTAAATCCTTCTGGGATAAGCCGTACACCATTGTTGAAAAAGATGGCGTCAAGATTGGC
GTAATCGGCTTACACGGTGTCTTTGCTTTTAATGATACGGTTTCCGCCGCGACGCGCGTAGGCATTGAGGCACGCGATGA
AATTAAGTGGCTGCAACGTTACATTGATGAACTTAAAGGTAAAGTCGATCTGACCGTCGCGCTGATTCACGAAGGCACCC
CGGCCCGCCAGTCCAGCATGGGAAATACCGATGTGCGACGCGCGCTGGATAAAGATATTCAGACCGCAAGTCAGGTAAAA
GGGCTGGATATTTTGATTACCGGCCACGCGCATGTCGGTACGCCGGAACCGATTAAAGTCGGTAATACGCTGATCCTCTC
AACGGACAGCGGCGGCATTGATGTCGGCAAACTGGTGCTGGACTACCAGGAAAAGCCGCATCAGTTTACGGTGAAGAACT
TCGAGCTGAAGACCATTTTTGCTGATGAGTGGAAGCCCGATCCGCAAACGAAACAGGTGATCGACGGCTGGAACAAAAAG
CTCGATAAAGTCGTGCAGCAGACGGTGGCCCAGTCGCCGGTTGAGTTGACCCGCGCGTATGGCGAATCGTCGTCGCTGGG
GAATCTGGCGGCGGATGCGCTGCTTTTTACGGCGGGGAAAGACACCCAGTTAGCGCTTACTAACTCTGGCGGTATCCGCA
ACGAAATCCCGGCTGGCGCGGTGACGATGGGGGCGGTAATCAGTACCTTCCCGTTCCCTAATGAACTGGTCACGATGGAT
TTAACCGGTAAACAATTGCGCAGCCTGATGGAGCATGGCGCCGGATTAAGCAACGGCGTATTGCAGGTGTCTAAAGGGCT
GGAGATGAAGTATGACAGCAGCAAACCTCTCGGCCAGCGGGTTACCGTGCTGACGCTCAATGGCAAACCGATTGACGATG
CGGCGGTTTATCACATCGCCACCAACAGCTTCCTTGCCGACGGCGGCGATGGTTTTGCGGCGTTCACGGAAGGCCAGGCG
CGGAATACCTCCGGCGGCTACTATGTGTCGAATGCGATAGTTGATTACTTTAAGGCGGGCAACACCATCACGGATGAGCA
GCTCAAAGGGATGCGCGTTGCGGATGTGAAGAAGTAA

Upstream 100 bases:

>100_bases
TGCCTTCATAAAACGGTACGCGGGTGTGAGGACTCTCACATAAAAAAACCTTTCAGAACGTAACGTAGCGAATCTTTATA
TGACTGAAAGGGACTTATTT

Downstream 100 bases:

>100_bases
TTTCCCCTGCCGCGGCGCGTTCAGTGTCGCGGCGTGACATGAAGAAAATTCAACTTTGCTGAATATTCCTCATTCTTTGG
CGTGATTTTTCAGTTGACGT

Product: 5'-Nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKVKLLAAGILFTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFT
GPYISSLTKGKAIIDILNTMQYDAATIGNHEFDHGWDNTLLQLSRAKFPIVQGNIFYEDSSKSFWDKPYTIVEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGTPARQSSMGNTDVRRALDKDIQTASQVK
GLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKNFELKTIFADEWKPDPQTKQVIDGWNKK
LDKVVQQTVAQSPVELTRAYGESSSLGNLAADALLFTAGKDTQLALTNSGGIRNEIPAGAVTMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSKGLEMKYDSSKPLGQRVTVLTLNGKPIDDAAVYHIATNSFLADGGDGFAAFTEGQA
RNTSGGYYVSNAIVDYFKAGNTITDEQLKGMRVADVKK

Sequences:

>Translated_518_residues
MKVKLLAAGILFTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFT
GPYISSLTKGKAIIDILNTMQYDAATIGNHEFDHGWDNTLLQLSRAKFPIVQGNIFYEDSSKSFWDKPYTIVEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGTPARQSSMGNTDVRRALDKDIQTASQVK
GLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKNFELKTIFADEWKPDPQTKQVIDGWNKK
LDKVVQQTVAQSPVELTRAYGESSSLGNLAADALLFTAGKDTQLALTNSGGIRNEIPAGAVTMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSKGLEMKYDSSKPLGQRVTVLTLNGKPIDDAAVYHIATNSFLADGGDGFAAFTEGQA
RNTSGGYYVSNAIVDYFKAGNTITDEQLKGMRVADVKK
>Mature_518_residues
MKVKLLAAGILFTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFT
GPYISSLTKGKAIIDILNTMQYDAATIGNHEFDHGWDNTLLQLSRAKFPIVQGNIFYEDSSKSFWDKPYTIVEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGTPARQSSMGNTDVRRALDKDIQTASQVK
GLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKNFELKTIFADEWKPDPQTKQVIDGWNKK
LDKVVQQTVAQSPVELTRAYGESSSLGNLAADALLFTAGKDTQLALTNSGGIRNEIPAGAVTMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSKGLEMKYDSSKPLGQRVTVLTLNGKPIDDAAVYHIATNSFLADGGDGFAAFTEGQA
RNTSGGYYVSNAIVDYFKAGNTITDEQLKGMRVADVKK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=531, Percent_Identity=26.5536723163842, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1786687, Length=506, Percent_Identity=24.1106719367589, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790658, Length=257, Percent_Identity=25.6809338521401, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI221329836, Length=540, Percent_Identity=26.2962962962963, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922446, Length=553, Percent_Identity=24.9547920433996, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24654424, Length=553, Percent_Identity=24.9547920433996, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19921980, Length=487, Percent_Identity=25.0513347022587, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652512, Length=487, Percent_Identity=25.0513347022587, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161076508, Length=487, Percent_Identity=25.0513347022587, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922444, Length=562, Percent_Identity=25.0889679715303, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24641187, Length=514, Percent_Identity=26.0700389105058, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28573524, Length=518, Percent_Identity=23.5521235521236, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56538; Mature: 56538

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKLLAAGILFTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLV
CEEEEEEHHHHHHHHHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHHHH
KQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDILNTMQYDAATIGNHEFDHGWDNTL
HHHHCCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCCCCHHH
LQLSRAKFPIVQGNIFYEDSSKSFWDKPYTIVEKDGVKIGVIGLHGVFAFNDTVSAATRV
HHHHHCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHHHC
GIEARDEIKWLQRYIDELKGKVDLTVALIHEGTPARQSSMGNTDVRRALDKDIQTASQVK
CCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCC
GLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKNFELKTIF
CEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEEEEEEE
ADEWKPDPQTKQVIDGWNKKLDKVVQQTVAQSPVELTRAYGESSSLGNLAADALLFTAGK
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCEEEEECCC
DTQLALTNSGGIRNEIPAGAVTMGAVISTFPFPNELVTMDLTGKQLRSLMEHGAGLSNGV
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCC
LQVSKGLEMKYDSSKPLGQRVTVLTLNGKPIDDAAVYHIATNSFLADGGDGFAAFTEGQA
EEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEEECCCCCEEEEECCCC
RNTSGGYYVSNAIVDYFKAGNTITDEQLKGMRVADVKK
CCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCEECCCCC
>Mature Secondary Structure
MKVKLLAAGILFTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLV
CEEEEEEHHHHHHHHHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHHHH
KQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDILNTMQYDAATIGNHEFDHGWDNTL
HHHHCCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCCCCHHH
LQLSRAKFPIVQGNIFYEDSSKSFWDKPYTIVEKDGVKIGVIGLHGVFAFNDTVSAATRV
HHHHHCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHHHC
GIEARDEIKWLQRYIDELKGKVDLTVALIHEGTPARQSSMGNTDVRRALDKDIQTASQVK
CCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCC
GLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKNFELKTIF
CEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEEEEEEE
ADEWKPDPQTKQVIDGWNKKLDKVVQQTVAQSPVELTRAYGESSSLGNLAADALLFTAGK
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCEEEEECCC
DTQLALTNSGGIRNEIPAGAVTMGAVISTFPFPNELVTMDLTGKQLRSLMEHGAGLSNGV
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCC
LQVSKGLEMKYDSSKPLGQRVTVLTLNGKPIDDAAVYHIATNSFLADGGDGFAAFTEGQA
EEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEEECCCCCEEEEECCCC
RNTSGGYYVSNAIVDYFKAGNTITDEQLKGMRVADVKK
CCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]