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The map label for this gene is tusA [H]

Identifier: 194735426

GI number: 194735426

Start: 3630465

End: 3630800

Strand: Reverse

Name: tusA [H]

Synonym: SeSA_A3770

Alternate gene names: 194735426

Gene position: 3630800-3630465 (Counterclockwise)

Preceding gene: 194736660

Following gene: 194738088

Centisome position: 77.1

GC content: 51.19

Gene sequence:

>336_bases
ATGGCTAATTTTCACGCAATCGTTAACCTGCCTGGCTTACTGACGAAGATGGGCGTAAAATATCGCCGTTTTTCTACCAT
TGAGACGAAGATGAGCGATCTGTTTTCCTCCCCTGACCATACGCTTGACGCCCTGGGGCTGCGTTGCCCTGAACCGGTAA
TGATGGTGCGCAAAACGGTGCGCAACATGCAAACTGGCGAAACATTGCTGATTATCGCCGACGATCCGGCGACAACGCGT
GATATTCCGGGGTTCTGTACCTTTATGGAACACGATTTGCTGGCGCAAGAGACGGAGGGTCTGCCGTACCGCTATTTATT
GCGTAAAGCGCATTAA

Upstream 100 bases:

>100_bases
TGATTGTGTAAACGGAGTCATATTGTGACCTTTTTGTTGGATGGGGTGAGGGAACCCAATAAAGAACCGCCGCATAATAC
TGTTTTGCGCTGGCATTGCA

Downstream 100 bases:

>100_bases
CCGCACGGCAGATAACATTCTGATTAGAAGCTTTCCCAGTTCGCCTGTTCTGCGGCGGGCTGTGGCGTTAAACCTTTGCT
TAACGGCGTTGGCGACGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MANFHAIVNLPGLLTKMGVKYRRFSTIETKMSDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQTGETLLIIADDPATTR
DIPGFCTFMEHDLLAQETEGLPYRYLLRKAH

Sequences:

>Translated_111_residues
MANFHAIVNLPGLLTKMGVKYRRFSTIETKMSDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQTGETLLIIADDPATTR
DIPGFCTFMEHDLLAQETEGLPYRYLLRKAH
>Mature_110_residues
ANFHAIVNLPGLLTKMGVKYRRFSTIETKMSDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQTGETLLIIADDPATTRD
IPGFCTFMEHDLLAQETEGLPYRYLLRKAH

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=80, Percent_Identity=90, Blast_Score=155, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 12612; Mature: 12481

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFHAIVNLPGLLTKMGVKYRRFSTIETKMSDLFSSPDHTLDALGLRCPEPVMMVRKTV
CCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
RNMQTGETLLIIADDPATTRDIPGFCTFMEHDLLAQETEGLPYRYLLRKAH
HCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
ANFHAIVNLPGLLTKMGVKYRRFSTIETKMSDLFSSPDHTLDALGLRCPEPVMMVRKTV
CCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
RNMQTGETLLIIADDPATTRDIPGFCTFMEHDLLAQETEGLPYRYLLRKAH
HCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA