Click here to switch to the map view.

The map label for this gene is citA [H]

Identifier: 194735213

GI number: 194735213

Start: 61798

End: 63456

Strand: Reverse

Name: citA [H]

Synonym: SeSA_A0059

Alternate gene names: 194735213

Gene position: 63456-61798 (Counterclockwise)

Preceding gene: 194737067

Following gene: 194735861

Centisome position: 1.35

GC content: 47.38

Gene sequence:

>1659_bases
ATGCAAGAAAATACCGTAATTAAATATCTAAATACCAATATGTTTATACGGGAAATTACACACTGGTTTGCCAGACGCTC
GTTTCAAAACCGGATATTTCTACTGATCCTGTTTACATCCACCATCGTGATGCTGGCAATCACCTGGTATCTGACCGATA
TCACGAAAGAGCGCCTGCATTATCAGGTGGGCCAACGTGCGCTTATTCAGGCAATGCAAATATCCGCTATGCCAGAATTA
GTTGAAGCCGTAGAGGCGCATGATTTGCCACGCATCAAAGCGCTGATTGACCCAATGCGCTCGTTTTCGGACGCGACTTA
CATTACCGTTGGCAACGAAAAAGGTCAGCGACTGTATCACGTCAACCCGAATGAGATCGGCAAATATATGGAGGGCGGCG
ATAGCGACGACGCACTGTATAACGCTAAAAGCTACGTATCGGTGCGCAAAGGTTCATTAGGCTCTTCCTTACGTGGTAAA
TCCCCGATTCAGGACAGTACCGGTAAAGTCATCGGTATTGTTTCCGTCGGCTATACTCTGGAACAGTTGGAGAGCTGGCT
TAACTTACAAATCAGCTCGTTGCTGTTCCCGATGGCGTTACTGCTGCTGGTACTGTTGTACTGCGCCCGCCGCTTCTCGC
TGCATATTAAAAAGCAGATGCTTAACATGGAGCCGCAGCAGCTTTCACAACTATTAATTCAGCAAAGCGTGTTGTTTGAG
TCGGTATTTGAAGGGTTGATCGCCATTGACTCCCATCACCGTATTACCGCCATTAACCAAACGGCTCGCCGCTTACTGAA
TCTGAGCCAACCGGAGTCTGAACTCATTGGCTATGCAATCCACCATATCGTGTTGCCGGAGGCTTTTTTTTACGATGCGC
CGCAAAAAAACAAAAAAGATGAGATCGTCACCTTTAACCAAATGAAAGTCATCGCCAGTCGCATGGCGGTCATTATTGAC
AACCAGCCTCAGGGCTGGGTTATTAGCTTTCGCAACAAAGACGATATCAATACACTCAGCCTGCAGTTAAGTCAGGTGCG
GCAATACGCCGATAACCTGCGCGTCGTGCAGCATGAGCATCGCAACCTTATTTCCACCATTGCCGGACTGCTGTTCCTGA
AGCGCTACGATAATGCGCTGGCGCTGATTCAGCAGCAATCGGAAAGCCACCAGAAAGTACTCGATTTTATTTCCCATAAT
TTCCGCGACCACCACCTTGCGGGGCTATTAATCGGTAAATATTATCGGGCAAAAGAGCTGGGGCTGGAGCTGATTTTCGA
TCCTTCAAGCTTTGTTGAACACCTGCCGTCATCGCTCTCGCATAATGAATGGATTTCGATTGTTGGCAATTTATTGGACA
ACGCCTATAACGCCACCCTGCTCAATCGCGAAGGCTCGCGACAGATTGAGTGCCTGATCAACAGCGAAGGTAATGAAGTC
ATTATTGAAGTTGCCGATCAGGGATGTGGTATTGACGAAAATATCCGCCAGCATATTTTCGAGCGTGGAGTGACCACCCA
CAACAACGGCGATCACGGAATTGGATTATGGTTGGTGCACAGCTACGTCAAGCAGGCCAATGGTGACATTATCGTCGATA
ACAATAATCCTTTTGGCACCATTTTTACCCTCTATATCCCTTTTACCAGAGAAGAATAA

Upstream 100 bases:

>100_bases
TGTAATAGTGGATGGGATACGCATGTCGTATCCCATTTTCATCCAAATTTTTTCTGAAAGAAAAGTAAGTATGAACTGGT
TCAGGTTCCGGTGATGCCGA

Downstream 100 bases:

>100_bases
CATGGATAGCATTACGACACTCATCGTTGAAGACGAACCGATGCTAGCGGAAATCCTGGTGGACACCATCAAGCTTTTTC
CCCAGTTTTCAATCGTCGGC

Product: sensor kinase DpiB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MQENTVIKYLNTNMFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPEL
VEAVEAHDLPRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPNEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGK
SPIQDSTGKVIGIVSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIID
NQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRVVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHN
FRDHHLAGLLIGKYYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE

Sequences:

>Translated_552_residues
MQENTVIKYLNTNMFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPEL
VEAVEAHDLPRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPNEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGK
SPIQDSTGKVIGIVSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIID
NQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRVVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHN
FRDHHLAGLLIGKYYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE
>Mature_552_residues
MQENTVIKYLNTNMFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPEL
VEAVEAHDLPRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPNEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGK
SPIQDSTGKVIGIVSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIID
NQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRVVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHN
FRDHHLAGLLIGKYYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=532, Percent_Identity=42.1052631578947, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790567, Length=532, Percent_Identity=28.9473684210526, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63000; Mature: 63000

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENTVIKYLNTNMFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLH
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YQVGQRALIQAMQISAMPELVEAVEAHDLPRIKALIDPMRSFSDATYITVGNEKGQRLYH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEE
VNPNEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTL
CCHHHHHHHHCCCCCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHH
EQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKD
HHHHHHHEECCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
EIVTFNQMKVIASRMAVIIDNQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRVVQHEH
CEEEHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKEL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
CEEEEECCHHHHHHCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEE
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGT
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEE
IFTLYIPFTREE
EEEEEEEECCCC
>Mature Secondary Structure
MQENTVIKYLNTNMFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLH
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YQVGQRALIQAMQISAMPELVEAVEAHDLPRIKALIDPMRSFSDATYITVGNEKGQRLYH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEE
VNPNEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTL
CCHHHHHHHHCCCCCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHH
EQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SVFEGLIAIDSHHRITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKD
HHHHHHHEECCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
EIVTFNQMKVIASRMAVIIDNQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRVVQHEH
CEEEHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKEL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEV
CEEEEECCHHHHHHCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEE
IIEVADQGCGIDENIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGT
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEE
IFTLYIPFTREE
EEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]