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The map label for this gene is nrfD [H]

Identifier: 194735175

GI number: 194735175

Start: 4409438

End: 4410394

Strand: Direct

Name: nrfD [H]

Synonym: SeSA_A4532

Alternate gene names: 194735175

Gene position: 4409438-4410394 (Clockwise)

Preceding gene: 194734388

Following gene: 194735440

Centisome position: 93.64

GC content: 59.14

Gene sequence:

>957_bases
ATGACACCCGCTTCTGCATTTCATTTTGCATCACTGGTGTGGGACTGGCCCATCGCCATCTATCTGTTCCTGATTGGCAT
CTCTGCCGGACTGGTTACGTTGGCGATTCTGCTGCGCCGCTTCCATCCTGAGGCGGGCGGTTCGGACAGCACGCTGCTGC
GCACCACGCTGGTGTTAGGGCCGGGGGCGATTATTCTTGGTCTGCTGATTCTGGTCTTTCACCTGACGCGCCCGTGGACC
TTCTGGAAGCTGATGTTCCACTACAGCTTTACCTCGGTGATGTCGATGGGGGTGATGCTGTTCCAGTTGTACATGGTAGT
GCTGGTGCTGTGGCTGGCGAAAATCTTTGAAAAAGAGGTGATTGCCCTGCAGCAGCGTTGGCTGCCGCGGTTAGAGATCG
TGCAGAAAGTGTTGGCGCTAATAACGCCGTTTCATCGCGCGCTGGAGACGTTAATGCTGGTGCTGGCGGTCCTGCTCGGG
GCGTACACCGGCTTTCTGCTGTCGGCGCTGAAATCCTATCCGTTCCTGAATAACCCGATCCTGCCGGTGCTGTTCCTGTT
CTCCGGCATCTCCTCCGGCGCGGCGGTGGCGCTGATCGCTATGGCGTTACGCCATCGTAGCAACCCGCACAGCACCGAGG
CGCGCTTTGTGCATCGCATGGAGGTTCCGGTGGTATGGCTGGAAATTTTCCTGCTGGCGGCGTTCTTCGTCGGTCTGGCG
CTGGGCGATGACGGCAAAATGCGCGCGCTGGCGGCGGCGCTGGGCGGCGGATTCTGGAGCTGGTGGTTCTGGCTTGGCGT
CGTAGGGCTGGGATTGATTATCCCGCTGCTGCTGAAACCCTGGGCAAATCGTAGCGTCACGTTTCACGGCGTGCTGGCCG
TTTGCGGCGCCAGTTTGACCGGCGTGCTGCTGCTGCGCTTTTTCATTCTTTATGCGGGACAGCTAACGGTTGCATAA

Upstream 100 bases:

>100_bases
CCCGTTTGTTGCGGCAAAAAACTACCTATCGTTACAAGCTGGCGCTGGGCACAAAACCGAAGGTCTACCGCGTTCCCTTT
AACTATGGGGAGGTGAGCCA

Downstream 100 bases:

>100_bases
TCTGGACATCTTCCTTCCTGAAGCCGGATTTCTGGCGCTGTTGTTGAGTCTCGGGGTCAACGTGTTGACCCCGCTGGCGA
CGTGGTGCGGGATGGGGCAG

Product: cytochrome c nitrite reductase, NrfD subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTPASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWT
FWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRLEIVQKVLALITPFHRALETLMLVLAVLLG
AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRMEVPVVWLEIFLLAAFFVGLA
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA

Sequences:

>Translated_318_residues
MTPASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWT
FWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRLEIVQKVLALITPFHRALETLMLVLAVLLG
AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRMEVPVVWLEIFLLAAFFVGLA
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA
>Mature_317_residues
TPASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWTF
WKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRLEIVQKVLALITPFHRALETLMLVLAVLLGA
YTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRMEVPVVWLEIFLLAAFFVGLAL
GDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=318, Percent_Identity=87.1069182389937, Blast_Score=552, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35245; Mature: 35114

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
PGAIILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALQQRWLPRLEIVQKVLALITPFHRALETLMLVLAVLLGAYTGFLLSALKSYPFLNNPI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LPVLFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRMEVPVVWLEIFLLAAFFVGLA
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLT
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GVLLLRFFILYAGQLTVA
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TPASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC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HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]