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The map label for this gene is entE [H]

Identifier: 194735160

GI number: 194735160

Start: 730765

End: 732375

Strand: Direct

Name: entE [H]

Synonym: SeSA_A0754

Alternate gene names: 194735160

Gene position: 730765-732375 (Clockwise)

Preceding gene: 194736755

Following gene: 194736615

Centisome position: 15.52

GC content: 57.67

Gene sequence:

>1611_bases
ATGCGTATACCTTTCACCCGTTGGCCGGATGAATTTGCCCGCCGTTATCGTGAAAAAGGCTACTGGCAAGACGTGCCGTT
GACCGATATTCTGACCCGCCACGCTGACAGCGATAAGACGGCGGTCATTGAAGGCGAGCGCGCATTCAGCTATCGCCAGC
TCAACCAGGCCGTGGATAATCTGGCCTGCAGTTTACGCCGTCAGGGCATCAAACCTGGCGAAACCGCTCTGGTGCAACTG
GGGAATGTACCGGAACTGTATATCACCTTTTTCGCCCTGTTGAAGCTTGGCGTAGCGCCCGTTCTGGCGCTGTTTAGCCA
TCAACGTACCGAACTTAACGCCTATGCGATGCAGATCGCGCCGACGCTGGTGATTGCCGATCGCCAACATACGCTGTTCG
CCGGGGAGGACTTTCTCAACACGTTTGTGGCTGAACACCGCTCTGTGCGGGTGGTGTTATTGCGCAACGACGACGGCGAT
CACAGCCTGGACGCGGCGATGCGGCAGGCGGCGGAAGATTTCACCGCCACGCCATCACCTGCTGACGAAGTGGCCTACTT
TCAGCTTTCCGGCGGTACTACCGGCACGCCAAAGCTTATTCCCCGTACCCATAACGACTATTACTACAGCGTGCGCCGCA
GCAATGAGATTTGCGGTTTCAACGAGGAGACGCGTTTTCTGTGCGCGATTCCCGCCGCGCATAACTACGCCATGAGTTCG
CCGGGCGCGCTGGGCGTTTTTCTCGCCAAAGGAACGGTAGTGCTGGCGACCGATCCCAGCGCCACGCTCTGTTTCCCGCT
GATCGAAAAACACCAGATTAATGCCACGGCGCTGGTGCCGCCCGCGGTCAGTCTATGGCTACAGGCTATCCAGGAGTGGG
GCGGCAATGCGCCGCTGGCGTCATTAAGGTTATTGCAGGTTGGCGGCGCGCGGCTTTCTGCGACGCTGGCCGCCCGTATT
CCGGCTGAAATTGGCTGTCAGTTGCAGCAGGTTTTCGGCATGGCGGAAGGGTTAGTGAACTATACCCGGCTGGACGATAG
TCCGGAACGGATTATCAATACCCAGGGAAGACCCATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGCCGACGGGAATC
CACTGCCGCCGGGCGAGATTGGTCGTCTGATGACGCGCGGCCCCTATACTTTTCGCGGCTATTTCAACAGTCCGCAACAC
AATGCCAGCGCCTTTGACGCCAACGGTTTTTACTGCTCCGGCGATCTGATCTCCATTGATCAAGACGGCTACATCACCGT
TCACGGGCGTGAAAAAGATCAGATCAATCGGGGCGGCGAGAAGATAGCCGCCGAAGAGATAGAAAATCTGTTACTGCGCC
ACCCGGCGGTGATCCATGCGGCGCTGGTCAGCATGGAAGATGAACTGCTGGGGGAAAAAAGTTGCGCATATCTGGTGGTA
AAAGAGCCGCTGCGAGCGGTACAGGTACGCCGTTTCCTGCGAGAGCAGGGCGTGGCGGAATTTAAATTACCGGATCGCGT
GGAGTGCGTTGCGTCACTGCCACTGACGCCGGTTGGTAAAGTCGATAAAAAACAATTACGCCAGCGGTTGGCGTCACGTT
CACCGCTCTGA

Upstream 100 bases:

>100_bases
CGGGATTGTCCCTGCCTCTTCGCCAGTCGGCGAGTGGCGGGAAACCGGCGTCAAGCTCTCTACGATGTTGAACGTTTTTG
GACTGCATTAAGGAACGATG

Downstream 100 bases:

>100_bases
AGGAGAAAGAGAGATGGCCATTCCGAAACTACAGTCTTACGCGCTGCCCACCGCACTGGATATCCCGACCAACAAAGTGA
ACTGGGCATTTGAGCCGGAG

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAVDNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Sequences:

>Translated_536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAVDNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
>Mature_536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAVDNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=486, Percent_Identity=24.2798353909465, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=24.7563352826511, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=23.7903225806452, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI58082049, Length=561, Percent_Identity=23.5294117647059, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI157311624, Length=563, Percent_Identity=23.0905861456483, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI157311622, Length=563, Percent_Identity=23.0905861456483, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI156151445, Length=192, Percent_Identity=31.25, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=23.5408560311284, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=23.6641221374046, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI187761345, Length=445, Percent_Identity=23.5955056179775, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI187761343, Length=445, Percent_Identity=23.5955056179775, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI8923896, Length=571, Percent_Identity=21.015761821366, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI253970489, Length=585, Percent_Identity=20.1709401709402, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=86.5168539325843, Blast_Score=962, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=32.3970037453184, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=26.4344262295082, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788107, Length=532, Percent_Identity=24.0601503759398, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786529, Length=540, Percent_Identity=23.5185185185185, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790505, Length=547, Percent_Identity=23.9488117001828, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=23.1607629427793, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786801, Length=527, Percent_Identity=23.1499051233397, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=545, Percent_Identity=26.4220183486239, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=27.2222222222222, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=25, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=25, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=25, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=525, Percent_Identity=24.5714285714286, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=528, Percent_Identity=25, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.6111111111111, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=535, Percent_Identity=22.0560747663551, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=23.0769230769231, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=351, Percent_Identity=27.3504273504274, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=23.4848484848485, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=534, Percent_Identity=22.2846441947566, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=534, Percent_Identity=22.2846441947566, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=366, Percent_Identity=24.8633879781421, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=26.5193370165746, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=565, Percent_Identity=22.8318584070796, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=25.0936329588015, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=23.3265720081136, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18859661, Length=375, Percent_Identity=24.5333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=525, Percent_Identity=23.047619047619, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=436, Percent_Identity=24.0825688073394, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=21.8436873747495, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=20.9259259259259, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24667955, Length=540, Percent_Identity=20.9259259259259, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=487, Percent_Identity=21.9712525667351, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648253, Length=476, Percent_Identity=22.4789915966387, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648255, Length=470, Percent_Identity=22.5531914893617, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=325, Percent_Identity=24.6153846153846, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358303, Length=342, Percent_Identity=23.0994152046784, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648260, Length=359, Percent_Identity=24.7910863509749, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667959, Length=127, Percent_Identity=31.496062992126, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648257, Length=390, Percent_Identity=23.8461538461538, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59285; Mature: 59285

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAVDN
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIA
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEC
PTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGDHSLDAAMRQAAEDFTATPSP
CEEEEECCCCEEECCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCHH
SLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMC
HEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
PDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCEEECCCEEEEC
QDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEE
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAVDN
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIA
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEC
PTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGDHSLDAAMRQAAEDFTATPSP
CEEEEECCCCEEECCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCHH
SLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMC
HEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
PDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCEEECCCEEEEC
QDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEE
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]