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The map label for this gene is rluE [H]

Identifier: 194735157

GI number: 194735157

Start: 1287244

End: 1287981

Strand: Reverse

Name: rluE [H]

Synonym: SeSA_A1316

Alternate gene names: 194735157

Gene position: 1287981-1287244 (Counterclockwise)

Preceding gene: 194737143

Following gene: 194737222

Centisome position: 27.35

GC content: 51.49

Gene sequence:

>738_bases
ATGTTTTGTAATGAGCATGTCAATACTACCCTATTGTTTGCCGCCATGAAAGACACTCATTATTGCGCTATAATGCGGCA
ATTAATAAGTTCTGAAAACACCATGCAAAAAACTTCTTTTAGAAATCACTACGTTAAGCGATTCAGCTCACGACAAGCAT
CTAAATCACGGAAAGAAAACCAGCCAAAACGCGTGGTTTTGTTCAATAAACCTTACGATGTGTTGCCGCAGTTCACGGAT
GAAGCAGGACGCCGCACGTTAAAAGATTTTATTCCTGTGCAAGGCGTTTATGCCGCAGGCCGCCTGGACCGCGACAGCGA
AGGGCTTTTGGTGCTGACTAATGACGGCGCGCTGCAGGCGCGCCTCACGCAACCGGGCAAGCGCACCGGCAAGATTTACT
ATGTTCAGGTAGAAGGCATCCCGGATAATGAAGCGCTACAGGCGCTTCGTACTGGCGTCACGCTCAACGATGGCCCTACC
CTGCCCGCCGGTATTGAGATTGTCGCGGAACCTGACTGGCTGTGGCCGCGCACCCCGCCGATTCGCGAACGGAAGAATAT
TCCCACCAGTTGGCTAAAAGTCACCTTATATGAAGGCCGCAACCGCCAGGTAAGACGCATGACTGCGCATGTCGGTCATC
CCACTCTACGATTAATTCGTTACAGCATGGGCGACTACACGCTCAGCGGCCTCGATAATGGACAGTGGCGCGAGATCGCA
CAGGAGAAAGACAGATGA

Upstream 100 bases:

>100_bases
TTCACCTCTCCTTCGAGCGCTTCTGGTTTGCTCGTCTGTTATCGCGCTGCATGTGCAATGGCGTCCTGCTGCACGACTGC
GTTAGAGCAATTTTTTGTTA

Downstream 100 bases:

>100_bases
AACGGCTTCTCATTGCTTTTGCTTTATTAACACCGTTATCCGTTAATGCGGCCAGCTTCGACTGCCAGAAAGCGCAGGCT
GCGGATGAAAAGGCTATCTG

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MFCNEHVNTTLLFAAMKDTHYCAIMRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTD
EAGRRTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNEALQALRTGVTLNDGPT
LPAGIEIVAEPDWLWPRTPPIRERKNIPTSWLKVTLYEGRNRQVRRMTAHVGHPTLRLIRYSMGDYTLSGLDNGQWREIA
QEKDR

Sequences:

>Translated_245_residues
MFCNEHVNTTLLFAAMKDTHYCAIMRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTD
EAGRRTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNEALQALRTGVTLNDGPT
LPAGIEIVAEPDWLWPRTPPIRERKNIPTSWLKVTLYEGRNRQVRRMTAHVGHPTLRLIRYSMGDYTLSGLDNGQWREIA
QEKDR
>Mature_245_residues
MFCNEHVNTTLLFAAMKDTHYCAIMRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTD
EAGRRTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNEALQALRTGVTLNDGPT
LPAGIEIVAEPDWLWPRTPPIRERKNIPTSWLKVTLYEGRNRQVRRMTAHVGHPTLRLIRYSMGDYTLSGLDNGQWREIA
QEKDR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=215, Percent_Identity=83.2558139534884, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787524, Length=183, Percent_Identity=37.1584699453552, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790453, Length=180, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788510, Length=186, Percent_Identity=31.1827956989247, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 28118; Mature: 28118

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCNEHVNTTLLFAAMKDTHYCAIMRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKEN
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPKRVVLFNKPYDVLPQFTDEAGRRTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQA
CCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RLTQPGKRTGKIYYVQVEGIPDNEALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPP
EECCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEECCCCCCCCCEEEEECCCCCCCCCCC
IRERKNIPTSWLKVTLYEGRNRQVRRMTAHVGHPTLRLIRYSMGDYTLSGLDNGQWREIA
CHHHCCCCCHHEEEEEECCCCCHHHHHHHHCCCHHHHHEEHHCCCEEECCCCCCHHHHHH
QEKDR
HHCCC
>Mature Secondary Structure
MFCNEHVNTTLLFAAMKDTHYCAIMRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKEN
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPKRVVLFNKPYDVLPQFTDEAGRRTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQA
CCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RLTQPGKRTGKIYYVQVEGIPDNEALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPP
EECCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEECCCCCCCCCEEEEECCCCCCCCCCC
IRERKNIPTSWLKVTLYEGRNRQVRRMTAHVGHPTLRLIRYSMGDYTLSGLDNGQWREIA
CHHHCCCCCHHEEEEEECCCCCHHHHHHHHCCCHHHHHEEHHCCCEEECCCCCCHHHHHH
QEKDR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]