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The map label for this gene is sgaE [H]

Identifier: 194735150

GI number: 194735150

Start: 4527414

End: 4528100

Strand: Direct

Name: sgaE [H]

Synonym: SeSA_A4656

Alternate gene names: 194735150

Gene position: 4527414-4528100 (Clockwise)

Preceding gene: 194734891

Following gene: 194738193

Centisome position: 96.14

GC content: 55.9

Gene sequence:

>687_bases
ATGCAAAAACTCAAGCAGCAGGTATTTGACGCTAACATGGATTTGCCCCGTTATGGACTGGTGACGTTTACCTGGGGCAA
CGTCAGCGCTATCGATCGCGAACGCGGGCTGGTGGTAATTAAGCCAAGCGGTGTCGCCTATGAAACCATGCAGGCAGATG
ACATGGTGGTGGTGGATATGGATGGCAAAGTGGTGGGGGGAGGTTATCGTCCTTCTTCCGATACCGCTACCCATCTGGCG
CTGTATCAACGCTATCCATCGCTTGGCGGCGTCGTTCATACCCACTCCACCCACGCCACGGCATGGGCGCAGGCGGGGAT
GGCTATTCCAGCGCTCGGCACCACGCACGCGGACTATTTCTTTGGCGATATTCCTTGTACCAGAGCGTTAAGCGAAGAAG
AAGTGCAGGGCGAGTACGAGCTGAACACCGGTAAGGTGATTATCGAAACGCTGGGCGAGGTGGAACCGCTGCATACGCCA
GGCATTGTCGTGTATCAACACGGGCCGTTCGCCTGGGGGAAAGATGCGCACGATGCGGTACATAACGCGGTCGTCATGGA
GGAAGTGGCGCGAATGGCGTGGATTGCGCGCGGTATTAACCCTGGCCTCAACCCCATCGACGATTATCTGATGAACAAGC
ACTTCATGCGTAAGCATGGCCCGAATGCGTATTACGGGCAGAAGTAA

Upstream 100 bases:

>100_bases
TGTGGAGCGAAACAGCAGAGAACCCGGCGGCAGAGGTAGCGAAAGCGCGTGACTGGGTGAAAGCGCGCATGGCCAGCGCC
GGTCTGGTGGAGGCAGCCTA

Downstream 100 bases:

>100_bases
AACAACGCTCCGGGATCCTATCCCTCAGGGATGTAATTGGTCAGATTCCGGAGCTTCTTTCATAACACTCTATAGAATCA
TACTACTGTGTCTATCAACG

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMQADDMVVVDMDGKVVGGGYRPSSDTATHLA
LYQRYPSLGGVVHTHSTHATAWAQAGMAIPALGTTHADYFFGDIPCTRALSEEEVQGEYELNTGKVIIETLGEVEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVARMAWIARGINPGLNPIDDYLMNKHFMRKHGPNAYYGQK

Sequences:

>Translated_228_residues
MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMQADDMVVVDMDGKVVGGGYRPSSDTATHLA
LYQRYPSLGGVVHTHSTHATAWAQAGMAIPALGTTHADYFFGDIPCTRALSEEEVQGEYELNTGKVIIETLGEVEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVARMAWIARGINPGLNPIDDYLMNKHFMRKHGPNAYYGQK
>Mature_228_residues
MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMQADDMVVVDMDGKVVGGGYRPSSDTATHLA
LYQRYPSLGGVVHTHSTHATAWAQAGMAIPALGTTHADYFFGDIPCTRALSEEEVQGEYELNTGKVIIETLGEVEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVARMAWIARGINPGLNPIDDYLMNKHFMRKHGPNAYYGQK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=91.6666666666667, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=59.5652173913044, Blast_Score=293, Evalue=6e-81,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=60, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI1789164, Length=138, Percent_Identity=36.231884057971, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25114; Mature: 25114

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMQADDMVVVDM
CHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCEEEEECCCCEEEEEC
DGKVVGGGYRPSSDTATHLALYQRYPSLGGVVHTHSTHATAWAQAGMAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRALSEEEVQGEYELNTGKVIIETLGEVEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCHHHCCCHHHCCCEEECCCCEEHEEHHCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVARMAWIARGINPGLNPIDDYLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMQADDMVVVDM
CHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCEEEEECCCCEEEEEC
DGKVVGGGYRPSSDTATHLALYQRYPSLGGVVHTHSTHATAWAQAGMAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRALSEEEVQGEYELNTGKVIIETLGEVEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCHHHCCCHHHCCCEEECCCCEEHEEHHCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVARMAWIARGINPGLNPIDDYLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA