Click here to switch to the map view.

The map label for this gene is thiI [H]

Identifier: 194735073

GI number: 194735073

Start: 489407

End: 490855

Strand: Direct

Name: thiI [H]

Synonym: SeSA_A0485

Alternate gene names: 194735073

Gene position: 489407-490855 (Clockwise)

Preceding gene: 194737031

Following gene: 194736804

Centisome position: 10.39

GC content: 53.07

Gene sequence:

>1449_bases
ATGAAGTTTATCATTAAATTGTTCCCGGAAATCACTATCAAAAGCCAATCTGTGCGTTTGCGCTTTATAAAAATTTTAAC
CGGGAACATCCGTAACGTTTTAAAGCACTACGATGAGACCCTCGCGGTTGTCCGTCACTGGGATAACATTGAAGTTCGCG
CCAAAGATGAAAACCAGCGTCTGGCGATTCGCGACGCGCTGACCCGCATTCCGGGGATTCACCATATTCTTGAAGTCGAA
GATGTGCCGTTCACCGATATGCACGACATTTTCGAGAAAGCGTTGGCGCAGTATCGCGAGCAGCTTGAAGGCAAAACCTT
CTGCGTGCGCGTAAAACGTCGCGGTAAGCATGAGTTTAGCTCCATTGAGGTGGAACGCTATGTCGGCGGCGGATTAAATC
AGCATATTGAATCGGCGCGCGTGAAGCTCACTAACCCGGATGTGACGGTGCATCTGGAAGTGGAAGATGATCGCCTGCTG
CTGATCAAAGGGCGTTATGAAGGTATTGGCGGTTTCCCGATTGGCACCCAGGAAGATGTGCTGTCGCTGATCTCCGGCGG
TTTTGACTCCGGCGTCTCCAGCTATATGCTGATGCGTCGCGGCTGCCGCGTACACTACTGCTTCTTTAACCTCGGCGGCG
CGGCGCATGAAATCGGCGTTCGCCAGGTGGCGCATTACCTGTGGAACCGCTTTGGCAGCTCCCATCGCGTGCGTTTTGTG
GCGATTAACTTCGAACCGGTGGTCGGCGAGATTCTGGAGAAAGTTGACGACGGCCAGATGGGCGTGGTGCTCAAACGTAT
GATGGTACGCGCGGCGTCGAAAGTGGCGGAACGTTACGGCGTACAGGCGCTGGTGACCGGCGAAGCGCTGGGCCAGGTGT
CCAGCCAGACGCTAACCAACTTGCGCTTGATCGATAACGTGTCTGACACGCTGATCCTGCGCCCGCTGATCTCTTACGAT
AAAGAGCACATTATCAACCTGGCGCGCCAGATTGGTACGGAAGATTTTGCCCGTACGATGCCGGAATACTGTGGCGTGAT
TTCAAAAAGTCCGACGGTGAAAGCCATTAAAGCGAAAATTGAAGCCGAAGAAGAAAATTTCGACTTCAGTATTCTCGATA
AGGTGGTAGAAGAAGCGAACAACGTCGATATTCGTGAAATCGCCCAGCAGACCCAGCAGGAGGTGGTGGAAGTAGAAACC
GTGAGCGGTTTTGGCGCCAACGATGTGATTCTGGATATCCGTTCTGTCGATGAGCAGGATGACAAGCCGCTGAAAGTGGA
AGGCGTCGACGTCGTTTCGCTGCCTTTCTACAAGCTGAGCACTAAATTTGGCGACCTCGATCAGAGCAAAACCTGGCTGC
TATGGTGCGAACGCGGCGTAATGAGTCGCCTGCAGGCGCTCTATCTGCGCGAGCAGGGGTTTGCCAATGTGAAGGTGTAT
CGTCCGTAA

Upstream 100 bases:

>100_bases
AGTGATATACTTCCGCGCCTGGATGCAGCCGTAGATATGGGCTGCTGTACTATTTCTCCACGACAAGCCTTAGATAGCTT
GCTAAAGAACCATTGCCGCC

Downstream 100 bases:

>100_bases
TTCCTTTGCCGGGGGGCGGCTATGTCCTACCCGGCCTACCGTTTTATTTGTGCCCCAACCGTTTTGCAATGCGCTCCAGC
TTCATCATCAGCAGCAGCGT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFSSIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKIEAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVET
VSGFGANDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFANVKVY
RP

Sequences:

>Translated_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFSSIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKIEAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVET
VSGFGANDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFANVKVY
RP
>Mature_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVE
DVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFSSIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYD
KEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKIEAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVET
VSGFGANDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFANVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=96.4730290456432, Blast_Score=937, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 54778; Mature: 54778

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQR
CCCCEEECCCCEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
LAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFS
EHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCHH
SIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDV
HEEEHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKI
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHEE
EAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVETVSGFGANDVILDIRSVDEQD
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCC
DKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFANVKVY
CCCEEEECCEEEECCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQR
CCCCEEECCCCEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCE
LAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFS
EHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCHH
SIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDV
HEEEHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFV
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKI
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHEE
EAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVETVSGFGANDVILDIRSVDEQD
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCC
DKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFANVKVY
CCCEEEECCEEEECCHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA