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The map label for this gene is basR [H]

Identifier: 194734993

GI number: 194734993

Start: 4424307

End: 4424975

Strand: Reverse

Name: basR [H]

Synonym: SeSA_A4547

Alternate gene names: 194734993

Gene position: 4424975-4424307 (Counterclockwise)

Preceding gene: 194737369

Following gene: 194733917

Centisome position: 93.97

GC content: 53.81

Gene sequence:

>669_bases
ATGAAGATACTGATTGTTGAAGACGACACGCTATTATTACAGGGGTTAATACTCGCCGCGCAAACCGAAGGCTATGCGTG
TGATGGCGTTTCGACAGCGCGTGCCGCCGAGCATAGTCTGGAGTCTGGTCATTACAGTCTGATGGTGCTGGATTTAGGGC
TGCCCGATGAGGATGGCCTGCATTTCCTGACGCGAATCCGACAGAAAAAATATACCCTCCCGGTACTCATTCTGACCGCC
CGCGATACGCTCAATGACCGCATTACCGGGCTGGATGTCGGCGCAGATGATTATCTGGTTAAACCCTTCGCCCTGGAGGA
GTTGCACGCCCGCATCCGCGCGCTGCTGCGCCGCCATAATAATCAGGGTGAAAGTGAACTGACGGTTGGCAATCTGACGC
TCAATATAGGCCGCCATCAGGCATGGAGGGATGGACAGGAACTGACCCTGACGCCTAAGGAGTACGCGCTGCTCTCACGG
TTGATGCTCAAGGCAGGCAGTCCGGTGCACCGGGAAATTCTTTATAACGATATCTACAACTGGGATAACGAACCCTCGAC
CAACACTCTGGAAGTGCATATACATAATTTGCGCGACAAAGTCGGCAAGTCGCGCATTCGCACGGTTCGCGGGTTTGGCT
ACATGCTGGTTGCCACTGAGGAAAGCTAA

Upstream 100 bases:

>100_bases
AGGACAATCTTTTCTCAACCATGCTGGGATTAACCGGCGTACAAACGACGTATTACCAGGCCGCAGATGATATTCTGCAA
CCGTGCAGGAGACTAAGCGA

Downstream 100 bases:

>100_bases
GTGAGCCTGATGCGTTTTCAGCGAAGAGCGATGACCCTTCGCCAGCGTTTAATGCTGACAATTGGTCTTATTCTGCTGGT
GTTCCAGTTAATCAGTACCT

Product: DNA-binding transcriptional regulator BasR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGLHFLTRIRQKKYTLPVLILTA
RDTLNDRITGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELTVGNLTLNIGRHQAWRDGQELTLTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVATEES

Sequences:

>Translated_222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGLHFLTRIRQKKYTLPVLILTA
RDTLNDRITGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELTVGNLTLNIGRHQAWRDGQELTLTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVATEES
>Mature_222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGLHFLTRIRQKKYTLPVLILTA
RDTLNDRITGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELTVGNLTLNIGRHQAWRDGQELTLTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVATEES

Specific function: Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=90.5405405405405, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=46.2962962962963, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=39.5555555555556, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=36.1990950226244, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.8078602620087, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=33.1818181818182, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI2367329, Length=235, Percent_Identity=37.8723404255319, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788394, Length=221, Percent_Identity=37.10407239819, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=32.8828828828829, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=32.5892857142857, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=28.2608695652174, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788550, Length=151, Percent_Identity=27.1523178807947, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790299, Length=133, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25054; Mature: 25054

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGL
CEEEEEECCHHHHHHHHEEEECCCEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCH
HFLTRIRQKKYTLPVLILTARDTLNDRITGLDVGADDYLVKPFALEELHARIRALLRRHN
HHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCC
NQGESELTVGNLTLNIGRHQAWRDGQELTLTPKEYALLSRLMLKAGSPVHREILYNDIYN
CCCCCEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
WDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVATEES
CCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGL
CEEEEEECCHHHHHHHHEEEECCCEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCH
HFLTRIRQKKYTLPVLILTARDTLNDRITGLDVGADDYLVKPFALEELHARIRALLRRHN
HHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCC
NQGESELTVGNLTLNIGRHQAWRDGQELTLTPKEYALLSRLMLKAGSPVHREILYNDIYN
CCCCCEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
WDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVATEES
CCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]