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The map label for this gene is wcaK [H]

Identifier: 194734951

GI number: 194734951

Start: 2204202

End: 2205482

Strand: Reverse

Name: wcaK [H]

Synonym: SeSA_A2334

Alternate gene names: 194734951

Gene position: 2205482-2204202 (Counterclockwise)

Preceding gene: 194734330

Following gene: 194735345

Centisome position: 46.83

GC content: 52.93

Gene sequence:

>1281_bases
ATGAAATTATTAATTTTAGGCAACCACACATGCGGCAACCGTGGTGATAGCGCCATTATGCGCGGTTTGCTTGATGCCAT
CCGTCAACAGGCGCCAGAGGCGGAGATGGATGTGATGAGCCGTTTCCCGGTGAGTTCCGCCTGGTTGCAGGGGCGCCCGA
TTATTGCCGACCCGCTGTATCAGTTAAGTCAGAAACAGCAGGCGGCGGCAGGACTTAACGGTCGGGTGAAAAAAGTATTA
CGCCGTCGTTTTCAGCATAAAATTTTATTGTCTAAAGTCGCCCAGGAAGGATCGCTGCGTAATTTTGCTATCGCGCCGGA
ATTTGCCGAATTCGCGCAATTTATTGCGCAGTATGACGCCGTCATTCAGGTGGGCGGTTCAAACTATGTCGACTTATACG
GTTTGACTCACTTTGAATATCCGCTGTGCGCGTTTATGGCGAACAAACCAATTTATATGGTGGGACATAGCGTCGGCCCT
TTCCAGAACCCTGACTTTAACCAGCTCGCCAATTATGTTTTTGGGCGCACCAGTGCGCTGATCCTGCGTGAAACCGTCAG
CCGCGATCTGATGACCGCCGCGCAGATCGATACCCGAAAAGTAGAGCAGGGGGTGGATACCGCCTGGTTAGTCGAACGCC
GTCACGACGAGTTTGTCGCCAGTTATGCTGTGCAGCACTGGCTGGACGTCACCGCGCGCCGCAAAACCGTCGCGATTACG
TTGCGCGATCTGGAGCCGTTTGATAAACGCCTGGGCACCACTCAGCAGGCCTATGAGCAGGCATTTGCCCAGGTCGTGAA
TCGCCTGATTGCGGACGGTTATCAGGTACTGGCGCTGTCGACCTGCACCAGTATCGATCGCTATAACCGCGATGACCGGA
TGGTTGCGTTACGGATGGCGAAGTATGTAAACGACTCAGAGCATTTTCATATCGCGATGGACGAACTCAATGACGTGGAA
ATCGGAAAGATCCTCGCATCCTGCGTACTGACCATCGGGACGCGCCTGCATTCCGCCATTATCTCCATGAATTTTGGCAC
GCCCGCCATCGCCATTAATTATGAACATAAATCCGCCGGGATTATGCAGCAGCTAGGCCTGGCGGAATTGGCTATCGATA
TCCGTCACTTGCTGAACGGTTCATTACAAAGCGTGGTGGCCGATACGCTCAATCAATTGCCTGCGCTAAAGGCAAAAGTG
GCTAACGCCGTTACCGAGGAGCGGGCGAAAGGGTTCACGATGATCAAATCCGTTCTCGACAGAATTCGGGAGGAACAATG
A

Upstream 100 bases:

>100_bases
ACGCAATGCGCTGGTGATGGAAATTAAAAATCAGCTTGTTGGCAGTGCAAAACTGAAAAAGTTATTACGTGTCGGATGAC
GGGGATATAAGAGGCTATTT

Downstream 100 bases:

>100_bases
AAGTCAGCTTTTTTCTGCTGAAATTTCCACTCTCATCGGAAACCTTTGTGCTGAATCAGATTACTGCGTTTGTTGATATG
GGCCATGAGGTGGAGATTGT

Product: putative pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREEQ

Sequences:

>Translated_426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREEQ
>Mature_426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSEHFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREEQ

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=424, Percent_Identity=68.1603773584906, Blast_Score=622, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47670; Mature: 47670

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCHHHHHH
QLSQKQQAAAGLNGRVKKVLRRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
VIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGPFQNPDFNQLANYVFGRTSAL
HEEECCCCEEEEECCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
ILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMA
EECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHHH
KYVNDSEHFHIAMDELNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAG
HHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHH
IMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKVANAVTEERAKGFTMIKSVLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIREEQ
HHHCCC
>Mature Secondary Structure
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCHHHHHH
QLSQKQQAAAGLNGRVKKVLRRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
VIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGPFQNPDFNQLANYVFGRTSAL
HEEECCCCEEEEECCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
ILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMA
EECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHHH
KYVNDSEHFHIAMDELNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAG
HHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHH
IMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKVANAVTEERAKGFTMIKSVLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIREEQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]