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The map label for this gene is hybD [H]

Identifier: 194734946

GI number: 194734946

Start: 1578147

End: 1578755

Strand: Reverse

Name: hybD [H]

Synonym: SeSA_A1640

Alternate gene names: 194734946

Gene position: 1578755-1578147 (Counterclockwise)

Preceding gene: 194734434

Following gene: 194735217

Centisome position: 33.53

GC content: 53.2

Gene sequence:

>609_bases
ATGGCAGAAGTAACAATCTTAGGGCTTGGCAACCTGCTTTGGGCAGATGAAGGGTTCGGCGTCAGAGCGGCGGAAAAGCT
GTTTGAACAGTATGCCGATAACGAGAAAGTTGACGTTGTTGATGGCGGTACGCAGGGACTGGCGCTGCTGCCCTGGCTGC
AACAGACCGAAAAACTGTTAATCATGGATGCTATCGATTTTGGTATGGCGCCAGGTTCGCTGGCGATGTTTCGTGATGAA
CAGGTTCCCGCCTATCTGACGGCGAAAAAACTCAGTTTACATCAGACCAGTTTTTCTGAAGTGCTGGCATTGCTGCAATT
AACCGGCGGCCAGTTATCGGAGATTGTGCTGATTGGCGTGCAGCCGGAATGTCTGGATGACTATGGCGGGAGTCTTACGC
CGCAGGTAAAGGCGCAACTGATGCCTGCGGTCTATCTCGCGCAGGAAGTGCTGGCGCAATGGGGTATCACCGCCTCTTCA
GCGGCCTTGCCCACTGAGAGACTCAATCATTACAGCCTGTGTATGGAACGCTACGAAGATGAACGGCCAGATGCGCAAAG
CGCTTGCCGGGTCGGCGATATTCGGGTGCTACAGAGAGAGAAATCATAA

Upstream 100 bases:

>100_bases
TCATTGCGCATGTTTATACCGCTATCCGCGAAGACATTATGAGCCGACAAAGCGTGATTTCCGTCATGATAAGCGGCTGG
CGCTGGTTCAGGTGATAACA

Downstream 100 bases:

>100_bases
TGTGTATTGGCGTTCCTGTGCAGGTTATTTCACCCGGTCAGTGGTTCGCCAAATGCCGCGACCGACACGGCGAGCTTATC
GACGTTGATATTCGGCTTGT

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAEVTILGLGNLLWADEGFGVRAAEKLFEQYADNEKVDVVDGGTQGLALLPWLQQTEKLLIMDAIDFGMAPGSLAMFRDE
QVPAYLTAKKLSLHQTSFSEVLALLQLTGGQLSEIVLIGVQPECLDDYGGSLTPQVKAQLMPAVYLAQEVLAQWGITASS
AALPTERLNHYSLCMERYEDERPDAQSACRVGDIRVLQREKS

Sequences:

>Translated_202_residues
MAEVTILGLGNLLWADEGFGVRAAEKLFEQYADNEKVDVVDGGTQGLALLPWLQQTEKLLIMDAIDFGMAPGSLAMFRDE
QVPAYLTAKKLSLHQTSFSEVLALLQLTGGQLSEIVLIGVQPECLDDYGGSLTPQVKAQLMPAVYLAQEVLAQWGITASS
AALPTERLNHYSLCMERYEDERPDAQSACRVGDIRVLQREKS
>Mature_201_residues
AEVTILGLGNLLWADEGFGVRAAEKLFEQYADNEKVDVVDGGTQGLALLPWLQQTEKLLIMDAIDFGMAPGSLAMFRDEQ
VPAYLTAKKLSLHQTSFSEVLALLQLTGGQLSEIVLIGVQPECLDDYGGSLTPQVKAQLMPAVYLAQEVLAQWGITASSA
ALPTERLNHYSLCMERYEDERPDAQSACRVGDIRVLQREKS

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=179, Percent_Identity=53.072625698324, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1789367, Length=156, Percent_Identity=39.7435897435897, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 22146; Mature: 22014

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVTILGLGNLLWADEGFGVRAAEKLFEQYADNEKVDVVDGGTQGLALLPWLQQTEKLL
CCEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHHHEE
IMDAIDFGMAPGSLAMFRDEQVPAYLTAKKLSLHQTSFSEVLALLQLTGGQLSEIVLIGV
EEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
QPECLDDYGGSLTPQVKAQLMPAVYLAQEVLAQWGITASSAALPTERLNHYSLCMERYED
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
ERPDAQSACRVGDIRVLQREKS
CCCCHHHHHHHCHHHEEECCCC
>Mature Secondary Structure 
AEVTILGLGNLLWADEGFGVRAAEKLFEQYADNEKVDVVDGGTQGLALLPWLQQTEKLL
CEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHHHEE
IMDAIDFGMAPGSLAMFRDEQVPAYLTAKKLSLHQTSFSEVLALLQLTGGQLSEIVLIGV
EEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
QPECLDDYGGSLTPQVKAQLMPAVYLAQEVLAQWGITASSAALPTERLNHYSLCMERYED
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
ERPDAQSACRVGDIRVLQREKS
CCCCHHHHHHHCHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624446 [H]