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The map label for this gene is cafA [H]

Identifier: 194734919

GI number: 194734919

Start: 3438052

End: 3439521

Strand: Reverse

Name: cafA [H]

Synonym: SeSA_A3562

Alternate gene names: 194734919

Gene position: 3439521-3438052 (Counterclockwise)

Preceding gene: 194733923

Following gene: 194735455

Centisome position: 73.04

GC content: 52.86

Gene sequence:

>1470_bases
ATGACGGCTGAATTGTTGGTAAACGTAACGCCATCGGAAACGCGCGTGGCGTACATTGATGGCGGTATTCTTCAGGAAAT
CCATATTGAGCGCGAAGCGCGACGCGGAATCGTAGGCAATATCTACAAAGGTCGTGTAAGTCGTGTACTTCCAGGTATGC
AGGCGGCTTTTGTAGATATTGGGCTGGATAAAGCCGCATTTCTTCATGCTTCCGACATTATGCCGCATACCGAATGCGTG
GCGGGCGACGAGCAAAAACAGTTTACGGTGCGCGATATCTCCGAACTGGTGCGTCAGGGACAGGATCTGATGGTGCAGGT
AGTGAAAGATCCGCTCGGCACGAAAGGCGCGCGTTTGACCACGGACATCACGTTGCCCTCCCGTTACCTTGTTTTTATGC
CCGGGGCGTCACACGTTGGCGTTTCCCAGCGTATCGAAAGCGAAAGTGAACGCGAGCGCCTGAAAAAGGTGGTGGCGGAA
TACTGCGACGAACAGGGCGGGTTTATTATCCGTACCGCGGCGGAAGGCGTGTGTGAAGAGGATCTCGCGTCTGACGCCGC
CTATCTCAAGCGCGTCTGGACGAAAGTCATGGAGCGCAAAAAGCGTCCGCAAACGCGCTACCAGATGTATGGCGAACTGG
CGCTGGCGCAGCGCGTGCTGCGCGATTTCGCCGATGCGCAGTTGGACCGAATTCGCGTCGACTCACGTCTGACCTATGAG
TCGCTGCTGGAGTTTACCGCAGAATACATCCCGGAAATGACCAGTAAGCTGGAGCATTACAGTGGCCATCAGCCGATCTT
CGATCTTTATGACGTGGAAAATGAAATCCAGCGCGCGCTGGAGCGTAAGGTTGAACTCAAGTCCGGCGGTTATCTGATTA
TCGACCAAACCGAAGCGATGACGACGGTAGATATCAATACCGGCGCGTTTGTCGGACACCGTAATCTCGACGACACCATT
TTTAATACCAATATTGAAGCGACTCAGGCCATTGCCCGCCAGCTACGCCTGCGTAATCTGGGCGGCATTATCATTATCGA
TTTTATCGACATGAATAATGAAGATCATCGCCGCCGGGTGCTGCATTCGCTGGAACAGGCGTTAAGTAAGGATCGCGTGA
AAACCAGTATTAACGGTTTTTCGCCGCTGGGGCTGGTGGAAATGACCCGTAAACGGACTCGTGAAAGCGTGGAACATGTG
CTTTGTAACGAGTGTCCAACCTGTCATGGCCGCGGGACGGTAAAAACGGTGGAAACCGTCTGCTATGAGATCATGCGTGA
AATTGTACGTGTTCATCACGCTTATGACTCCGATCGCTTCCTGGTTTATGCTTCTCCTGCGGTGGCGGAAGCGCTGAAAG
GCGAAGAATCACACGCGCTGGCGGAAGTCGAAATCTTTGTCGGCAAACAGGTTAAAGTTCAGGTCGAACCGCTTTATAAC
CAGGAGCAGTTTGACGTCGTAATGATGTAA

Upstream 100 bases:

>100_bases
AAGATAAATGGCAGCTATCACGCCGTGGTCGGCTTACCGCTGGTTGAAACGTATGAGTTGTTGAGTCATTTTAACGCACT
GCGTGATAAAAGGGATAAAC

Downstream 100 bases:

>100_bases
TTGAGTAGTAGCACCAGCGCATGTAGGCCGGATAAGGCGCAAGCGCCGCCATCCGGCAATATACCTTCAAGGGGTGTGAG
TCGTATTTTTTGGCAGACAA

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
YCDEQGGFIIRTAAEGVCEEDLASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYE
SLLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQVEPLYN
QEQFDVVMM

Sequences:

>Translated_489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
YCDEQGGFIIRTAAEGVCEEDLASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYE
SLLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQVEPLYN
QEQFDVVMM
>Mature_488_residues
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVA
GDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEY
CDEQGGFIIRTAAEGVCEEDLASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYES
LLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIF
NTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHVL
CNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQVEPLYNQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=96.7280163599182, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=436, Percent_Identity=36.2385321100917, Blast_Score=268, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55437; Mature: 55306

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIMPHTECVAGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEYCDEQGGFIIRTAAEGVCEE
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
DLASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SLLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQVEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCCEEEC
>Mature Secondary Structure 
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIMPHTECVAGDEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEYCDEQGGFIIRTAAEGVCEE
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
DLASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SLLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHALAEVEIFVGKQVKVQVEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]