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The map label for this gene is rsmD [H]

Identifier: 194734826

GI number: 194734826

Start: 3624276

End: 3624899

Strand: Direct

Name: rsmD [H]

Synonym: SeSA_A3764

Alternate gene names: 194734826

Gene position: 3624276-3624899 (Clockwise)

Preceding gene: 194736809

Following gene: 194737556

Centisome position: 76.96

GC content: 56.73

Gene sequence:

>624_bases
TTGCAACAGGCGGTTCGAGCAAGAGAAATGAAAAAACCGACTCACTCTGGCAGCGGCCAAATCCGCATTATTGGCGGGCA
ATGGCGTGGCCGTAAATTACCGGTTCCGGACAGCCCGGGTCTGCGTCCCACCACCGATCGCGTGCGCGAAACGTTATTTA
ACTGGCTGGCTCCGGTAATGGTAGACGCCCATTGTCTGGATTGCTTTGCCGGCAGCGGCGCTCTGGGCCTGGAGGCGCTA
TCGCGTTATGCGGCCCAGGCAACGTTACTGGAAATGGATCGCGCCGTATCGCAGCAGTTGCAAAAAAATCTGGCGACGCT
GAAAGCCAACAATGCTCGCGTCGTCAATACCAATACTCTGGCCTTCCTCAGCCAGCCGGGAACGCCGCATCACGTCGTCT
TTGTCGACCCGCCGTTTCGTAAAGGGCTGCTGGAAGAGACGTTACAATTGCTGGAAACCCAGGGATGGCTGGCCGACGAC
GCGCTAATCTACGTAGAAAGCGAGGTGGAAAACGGTCTGCCGCCCGTTCCGGCGAACTGGGCATTGTATCGAGAAAAGGT
GGCCGGACAGGTCGCTTATCGTCTGTATCAGCGCGACGCGCAAGGAGAAAATGATGTTGATTAA

Upstream 100 bases:

>100_bases
ATCTGCGACTACACCCCTCGCGGCGCTATATACATGGAAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTC
ACATCACCGCCTGGAATAAC

Downstream 100 bases:

>100_bases
TCTGGGCCGCTTATTAATGCTCTTCGTGTGGGCATTTTTGATTCTTAATCTGGTGCAACCCTTCCCGCGCCCGCTCAATA
TCTTCGTCAACGTGGCGTTG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQQAVRAREMKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEAL
SRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADD
ALIYVESEVENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD

Sequences:

>Translated_207_residues
MQQAVRAREMKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEAL
SRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADD
ALIYVESEVENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD
>Mature_207_residues
MQQAVRAREMKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEAL
SRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADD
ALIYVESEVENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=198, Percent_Identity=86.3636363636364, Blast_Score=362, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22928; Mature: 22928

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQAVRAREMKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVM
CCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VDAHCLDCFAGSGALGLEALSRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEE
AFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEVENGLPPVPANW
EEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCCCCCCH
ALYREKVAGQVAYRLYQRDAQGENDVD
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQQAVRAREMKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVM
CCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VDAHCLDCFAGSGALGLEALSRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEE
AFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEVENGLPPVPANW
EEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCCCCCCH
ALYREKVAGQVAYRLYQRDAQGENDVD
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]