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The map label for this gene is pucK [H]

Identifier: 194734802

GI number: 194734802

Start: 3700560

End: 3701885

Strand: Reverse

Name: pucK [H]

Synonym: SeSA_A3829

Alternate gene names: 194734802

Gene position: 3701885-3700560 (Counterclockwise)

Preceding gene: 194734273

Following gene: 194735610

Centisome position: 78.61

GC content: 55.28

Gene sequence:

>1326_bases
ATGCCGCAACCTGGCCTTACTACGCCGGATAACGATGCATTACCCTGGCAAACCACCTTACTTTTGGCGCTTCAGCATGT
GCTGGTCGTTGCCGCCACCCCCATTACCTCCGTCTTCCTGATCGCCAAAGCGCTGCACTTTACCGATACGGTGACCGCCT
CGGTACTTAGCGCGACCTTTCTGATGTGCGGTCTGGGCGCTATTTTGCAAAGCCTGGGTGTGAAAGGGGTGGGCGCGCGT
TTGCCGTTTATTATGGTACCGGGGGGCGCGCCGATTGCGATTTTCGTTGCGATCGCTTTACAGACCAATATCCAGACCGC
GATTGGCGCCGTAATTCTGACCTCGCTGTTCTACTTTATCGCGTTGCCGATTTTCCGCCGCTGCCTGCACCATTTTCCCC
CCTTTATCATTGGCATCATGCTGCTAATGGTGTCGATTAACCTTATTCGCCTGTACGGCGGGTTGATCATTGGCCAGCCA
GGGAGCGCCGACTTCGCTCATCCAACCAGTATCATTTTATCGCTGGGGACTATCCTGATAACGCTGATTTTCGCGCTGGC
GTTTAGCGGTATTTTGCGCCAGTTGGCGGTGATGTTCGGCCTTTTAGCGGGGACGTTGCTGGGAATGGCGCTGGGCAGCA
CCGATTTTAGCGGTGTTGGTCACGGGCCGCTGTTTAGTTTCCCACAGCTTTTGCCGTTCGGCTGGCCGATTTTTGATCTC
TCCGCCTCGCTGCCGTTACTGATTTACGCGGTCATCTCTATGGCTGAAGCAACCGGGCAAACGATTGCGACCGCGGAAAT
AGTCAATTCTACGCAAAATGTGCAGCAGGCGATTCCACGCACCATTCGTGGTGATGCGGTAATGTCGCTGCTGGGGGGGA
TTTTTGGCACCTCCTTAATTATCACCTCCGGCGAAAATATTGGGGTGGTACGCACCACTAATGTGAAATCGCGTTTCGTG
ACTGCGGCTGCCGGAGGGTTGTTAATCCTGATTGCCATATTCGCGCCGCTGGTACGTCTGGCGACCTGTTTGCCAGGTTC
GGTGGTGTGCGGTACGGCGGTGATTGTCTTCAGCATTATCGGGGTCATCGGCATTGATATGATTGCGCGTGAGCCACTGC
ATACGCCGGGTAAAACCTACGCGCTGGCGATGGGGCTGGCCATGGGAATGCTACCTATACTGGTACCCGGCCTGTATCAG
AATTTCCCGGCGGGCGTACAGATGGTCTTTGGCAACGGCATGGCAGCGGGGACGTTGACAGCGATTCTGGTGAACTCGTT
GTTTAACTGGAGCGAAAAGCGCACGCAGGCCAGGGTCAAAAGTTAG

Upstream 100 bases:

>100_bases
TGCGCATCGCTATGCCGCTGATGCCATTAGTTGGTTGAAATAATGCAGACCGCCAGCCCGCGCTGGCGGTTATTTTTTTG
ATGTTTTATGGGAGTTAATC

Downstream 100 bases:

>100_bases
TTTTTGTGATCGCGGTAGGCCGCACGACGGCCTACTTATTTACAATTTTGTGACATATTCGATGGTGTTTTTCAATGTCC
GGATAACTCTTTTTTGCACA

Product: xanthine/uracil/vitamin C permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPQPGLTTPDNDALPWQTTLLLALQHVLVVAATPITSVFLIAKALHFTDTVTASVLSATFLMCGLGAILQSLGVKGVGAR
LPFIMVPGGAPIAIFVAIALQTNIQTAIGAVILTSLFYFIALPIFRRCLHHFPPFIIGIMLLMVSINLIRLYGGLIIGQP
GSADFAHPTSIILSLGTILITLIFALAFSGILRQLAVMFGLLAGTLLGMALGSTDFSGVGHGPLFSFPQLLPFGWPIFDL
SASLPLLIYAVISMAEATGQTIATAEIVNSTQNVQQAIPRTIRGDAVMSLLGGIFGTSLIITSGENIGVVRTTNVKSRFV
TAAAGGLLILIAIFAPLVRLATCLPGSVVCGTAVIVFSIIGVIGIDMIAREPLHTPGKTYALAMGLAMGMLPILVPGLYQ
NFPAGVQMVFGNGMAAGTLTAILVNSLFNWSEKRTQARVKS

Sequences:

>Translated_441_residues
MPQPGLTTPDNDALPWQTTLLLALQHVLVVAATPITSVFLIAKALHFTDTVTASVLSATFLMCGLGAILQSLGVKGVGAR
LPFIMVPGGAPIAIFVAIALQTNIQTAIGAVILTSLFYFIALPIFRRCLHHFPPFIIGIMLLMVSINLIRLYGGLIIGQP
GSADFAHPTSIILSLGTILITLIFALAFSGILRQLAVMFGLLAGTLLGMALGSTDFSGVGHGPLFSFPQLLPFGWPIFDL
SASLPLLIYAVISMAEATGQTIATAEIVNSTQNVQQAIPRTIRGDAVMSLLGGIFGTSLIITSGENIGVVRTTNVKSRFV
TAAAGGLLILIAIFAPLVRLATCLPGSVVCGTAVIVFSIIGVIGIDMIAREPLHTPGKTYALAMGLAMGMLPILVPGLYQ
NFPAGVQMVFGNGMAAGTLTAILVNSLFNWSEKRTQARVKS
>Mature_440_residues
PQPGLTTPDNDALPWQTTLLLALQHVLVVAATPITSVFLIAKALHFTDTVTASVLSATFLMCGLGAILQSLGVKGVGARL
PFIMVPGGAPIAIFVAIALQTNIQTAIGAVILTSLFYFIALPIFRRCLHHFPPFIIGIMLLMVSINLIRLYGGLIIGQPG
SADFAHPTSIILSLGTILITLIFALAFSGILRQLAVMFGLLAGTLLGMALGSTDFSGVGHGPLFSFPQLLPFGWPIFDLS
ASLPLLIYAVISMAEATGQTIATAEIVNSTQNVQQAIPRTIRGDAVMSLLGGIFGTSLIITSGENIGVVRTTNVKSRFVT
AAAGGLLILIAIFAPLVRLATCLPGSVVCGTAVIVFSIIGVIGIDMIAREPLHTPGKTYALAMGLAMGMLPILVPGLYQN
FPAGVQMVFGNGMAAGTLTAILVNSLFNWSEKRTQARVKS

Specific function: Uptake of uric acid [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082181, Length=426, Percent_Identity=26.5258215962441, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082178, Length=434, Percent_Identity=24.8847926267281, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788843, Length=243, Percent_Identity=26.3374485596708, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790087, Length=442, Percent_Identity=25.1131221719457, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081818, Length=412, Percent_Identity=25, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 46144; Mature: 46013

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQPGLTTPDNDALPWQTTLLLALQHVLVVAATPITSVFLIAKALHFTDTVTASVLSATF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMCGLGAILQSLGVKGVGARLPFIMVPGGAPIAIFVAIALQTNIQTAIGAVILTSLFYFI
HHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPIFRRCLHHFPPFIIGIMLLMVSINLIRLYGGLIIGQPGSADFAHPTSIILSLGTILI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHH
TLIFALAFSGILRQLAVMFGLLAGTLLGMALGSTDFSGVGHGPLFSFPQLLPFGWPIFDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHH
SASLPLLIYAVISMAEATGQTIATAEIVNSTQNVQQAIPRTIRGDAVMSLLGGIFGTSLI
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
ITSGENIGVVRTTNVKSRFVTAAAGGLLILIAIFAPLVRLATCLPGSVVCGTAVIVFSII
EECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GVIGIDMIAREPLHTPGKTYALAMGLAMGMLPILVPGLYQNFPAGVQMVFGNGMAAGTLT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
AILVNSLFNWSEKRTQARVKS
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]