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The map label for this gene is phnU [H]

Identifier: 194734791

GI number: 194734791

Start: 491699

End: 492559

Strand: Reverse

Name: phnU [H]

Synonym: SeSA_A0487

Alternate gene names: 194734791

Gene position: 492559-491699 (Counterclockwise)

Preceding gene: 194734712

Following gene: 194737201

Centisome position: 10.46

GC content: 56.91

Gene sequence:

>861_bases
ATGTCACTCATTTTACCTCTGGAAAAACCGGCGCTTAACCTACGCCCGCTGCTGTGGCTGCTGCTCCCGTTGCTGGTGCT
GGCGACACTGTTTTTCTGGCCTCTGTCGCTCATCGTTGAACAGGCGCTACGCGGTGCCAATGGCGAGATCGGGCTCGAGA
CCTTCCGCCAGGTGGTCGACTCGAAACGCTTTGTCGGCGCGTTGCTGAACACGTTGCAAATCGCCTTTTTCGCCACCGCC
GGTTGCCTGCTGCTGGGCAGCGTGATGTCGCTGATTCTGGTGTTTATTCCTTTCCCCGGCAGCGAGTGGATTGGTTGGGT
CGTCGATACTTTTATTGCGCTGCCGACCTTCCTGATTACGCTGGCGTTCACCTTTATTTATGGGTCAGCGGGTCTGCTCA
ACGGTACGCTGATGAGCCTGTTTGCCTTTGAGCTACCGCCGGTAGATTTTCTCTACTCGATGCAGGGCGTCATTCTGGCG
GAGATCACGGTGTTCACTCCTCTGGTGATGCGGCCACTGATGGCCGCGCTGCGGCAAATTGATAAAAGCCAGCTAGAAGC
AGCCAGCATTCTGGGGGCGCACCCGTTACGGGTGATTGGGCAGGTGATTTTCCCGGCGGCGCTACCGGCGCTGATGGCTG
GTGGCAGCCTGTGCCTGCTGTTGACCACTAACGAGTTCGGCATCGTACTGTTTATTGGCGCGAAAGGGGTTAACACCCTG
CCGATGATGGTTTACAGCAAAGCGATTCTGGAGTCCGACTATACGGTCGCCTGCATGATTGCGTTGATTAACATTGTCTT
GTCGCTGGGGCTGTTCTCCCTCTATCGCCTGGCGGCATCACGTACCGGCGTAAGGAGCTAG

Upstream 100 bases:

>100_bases
TGCGGATGGTGTTGACGCATGTCAATCCGCTGCCGCGCGTGGGCGACAAGCTGGCGCTGTGGTTCGAACCGGATGATGCG
GTCCTGATTGAGGTATAACC

Downstream 100 bases:

>100_bases
CCATGCTAATTTGGTCACCTAAAGGACGCGCAGCGGCGGGCGTTGTGGCTAGCGTACTGTTTATCGTGTTCTTTTTCCTG
CCGCTGGCGGTGATTCTGAT

Product: 2-aminoethylphosphonate ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSLILPLEKPALNLRPLLWLLLPLLVLATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATA
GCLLLGSVMSLILVFIPFPGSEWIGWVVDTFIALPTFLITLAFTFIYGSAGLLNGTLMSLFAFELPPVDFLYSMQGVILA
EITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMAGGSLCLLLTTNEFGIVLFIGAKGVNTL
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRS

Sequences:

>Translated_286_residues
MSLILPLEKPALNLRPLLWLLLPLLVLATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATA
GCLLLGSVMSLILVFIPFPGSEWIGWVVDTFIALPTFLITLAFTFIYGSAGLLNGTLMSLFAFELPPVDFLYSMQGVILA
EITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMAGGSLCLLLTTNEFGIVLFIGAKGVNTL
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRS
>Mature_285_residues
SLILPLEKPALNLRPLLWLLLPLLVLATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATAG
CLLLGSVMSLILVFIPFPGSEWIGWVVDTFIALPTFLITLAFTFIYGSAGLLNGTLMSLFAFELPPVDFLYSMQGVILAE
ITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMAGGSLCLLLTTNEFGIVLFIGAKGVNTLP
MMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRS

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=26.8085106382979, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788764, Length=219, Percent_Identity=26.9406392694064, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017636
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30995; Mature: 30864

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLILPLEKPALNLRPLLWLLLPLLVLATLFFWPLSLIVEQALRGANGEIGLETFRQVVD
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SKRFVGALLNTLQIAFFATAGCLLLGSVMSLILVFIPFPGSEWIGWVVDTFIALPTFLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LAFTFIYGSAGLLNGTLMSLFAFELPPVDFLYSMQGVILAEITVFTPLVMRPLMAALRQI
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKSQLEAASILGAHPLRVIGQVIFPAALPALMAGGSLCLLLTTNEFGIVLFIGAKGVNTL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCHH
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLILPLEKPALNLRPLLWLLLPLLVLATLFFWPLSLIVEQALRGANGEIGLETFRQVVD
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SKRFVGALLNTLQIAFFATAGCLLLGSVMSLILVFIPFPGSEWIGWVVDTFIALPTFLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LAFTFIYGSAGLLNGTLMSLFAFELPPVDFLYSMQGVILAEITVFTPLVMRPLMAALRQI
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKSQLEAASILGAHPLRVIGQVIFPAALPALMAGGSLCLLLTTNEFGIVLFIGAKGVNTL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCHH
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA