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The map label for this gene is rumB [H]

Identifier: 194734709

GI number: 194734709

Start: 1006242

End: 1007369

Strand: Direct

Name: rumB [H]

Synonym: SeSA_A1035

Alternate gene names: 194734709

Gene position: 1006242-1007369 (Clockwise)

Preceding gene: 194734063

Following gene: 194737680

Centisome position: 21.37

GC content: 55.14

Gene sequence:

>1128_bases
ATGCAGTGCGCACTCTATGACGCGGGTCGCTGCCGTTCCTGTCAATGGATTACGCAGTCGGTTAATGAACAACTTTCCGC
AAAAACAGCCGATTTACATCGTTTGCTGACCGGGCTGCCTGTTGAACAGTGGTGCGCGTCGATTGGCGGCCCGGAACAGC
ATTTCCGCAACAAAGCGAAAATGGTCGTGAGCGGTAGTGTAGAAAAACCGCTCTTCGGGATGCTGCATCGCGACGGTACG
CCAGTCGATCTTTGCGGCTGCCCGCTGTATCCCGCCTCCTTTGCCCCCGTATTCAGCGCGCTAAAACCTTTTATTGCCCG
TGCCGGACTCACGCCTTATAACGTGGCGCGAAAACGTGGCGAACTGAAATACCTGTTGCTGACGGAAAGTCAGTTCGATG
GCGGCATGATGCTACGCTTCGTGTTGCGTTCAGAAACCAAACTTACGCAACTTCGCGCAGCGTTACCGTGGCTGCGTGCG
CAACTGCCGCAGCTCAGGGTGATCACCGCAAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAAATCTA
TCTGACGGATCAGCAGGCGCTGGCGGAACGTTTTAACGATGTGCCGCTGTGGATTCGTCCGCAAAGTTTCTTTCAGACCA
ACCCTACGGTCGCCAGCCGATTATATGCAACGGCGCGCGATTGGGTAGGGCAACTGCCGGTACGCCATATGTGGGATCTT
TTTTGCGGAGTGGGCGGATTCGGCCTGCACTGCGCGACGCCGCAGATGCAATTAACCGGTATTGAGATCGCGCCGGAGGC
GATTGCCTGCGCGAAGCAATCCGCTGCCGAGCTGGGATTGACCCGCCTTCATTTCCAGGCGTTGGATTCCACCCAATTCG
CCACCGCACAAGGCGAAACGCCCGACCTGGTGTTGGTGAATCCTCCCCGCCGTGGCATTGGCAAGCCGTTGTGCGATTAC
CTTGCACAAATGGCGCCGCGTTTTATTATTTATTCAAGCTGTAACGCGCAGACGATGGCGCAGGATATTCGCCATCTGCC
GAACTACCGTATTCAAAGTGTTCAGCTTTTCGATATGTTCCCGCATACCGCGCATTATGAAGTGTTAACATTGCTTTGCA
GGCTGTAG

Upstream 100 bases:

>100_bases
AATTCTGTTTCTGCTGTTTATTCCCGCTCCCAGCCGTCGGTTCTTCCGGCTGCAATAATGTGTATAATCGTCGCCCCGGA
TAATATGAAGGTTTTTTGGT

Downstream 100 bases:

>100_bases
CGCCAAAAGCCAGACATACAAAATATCTATATTAACTGTCTAATTATCCATAAATAATAATAACCATGATCGATCGATAA
AAAACTCCGATCGATCATGG

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLTGLPVEQWCASIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFAPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQSVQLFDMFPHTAHYEVLTLLCRL

Sequences:

>Translated_375_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLTGLPVEQWCASIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFAPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQSVQLFDMFPHTAHYEVLTLLCRL
>Mature_375_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLTGLPVEQWCASIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFAPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQSVQLFDMFPHTAHYEVLTLLCRL

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=186, Percent_Identity=28.494623655914, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI51173878, Length=186, Percent_Identity=28.494623655914, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=87.1657754010695, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=384, Percent_Identity=26.8229166666667, Blast_Score=101, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24666579, Length=368, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41977; Mature: 41977

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLTGLPVEQWCASIGGPEQHFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCHHE
MVVSGSVEKPLFGMLHRDGTPVDLCGCPLYPASFAPVFSALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRAQLPQLRVITANIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEE
EGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
CCCEEEEEECHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGET
HHCCCCCCEEECCCCEEEECEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCC
PDLVLVNPPRRGIGKPLCDYLAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQSVQLFDMF
CCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECC
PHTAHYEVLTLLCRL
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLTGLPVEQWCASIGGPEQHFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCHHE
MVVSGSVEKPLFGMLHRDGTPVDLCGCPLYPASFAPVFSALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRAQLPQLRVITANIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEE
EGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
CCCEEEEEECHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGET
HHCCCCCCEEECCCCEEEECEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCC
PDLVLVNPPRRGIGKPLCDYLAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQSVQLFDMF
CCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECC
PHTAHYEVLTLLCRL
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA