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The map label for this gene is fcl [H]

Identifier: 194734429

GI number: 194734429

Start: 2213179

End: 2214144

Strand: Reverse

Name: fcl [H]

Synonym: SeSA_A2341

Alternate gene names: 194734429

Gene position: 2214144-2213179 (Counterclockwise)

Preceding gene: 194738147

Following gene: 194736908

Centisome position: 47.02

GC content: 59.73

Gene sequence:

>966_bases
ATGAATAAGCAACGAATTTTTGTGGCGGGCCATCGTGGGATGGTGGGATCCGCCATTGTACGGCAGCTTGCGCAGCGCGG
CGACGTGGAACTGGTACTGCGCACCCGCGATGAGCTGGATCTGCTCGACGGGCGTGCGGTACAGGCGTTCTTTGCCGGGG
CGGGTATCGACCAGGTTTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTCGCCAACAACACGTATCCGGCGGATTTTATT
TATGAAAACATGATGATAGAGAGCAACATTATTCACGCCGCGCACCTGCACAACGTGAACAAACTGCTGTTTCTCGGTTC
GTCCTGTATCTATCCGAAACTGGCAAGGCAGCCGATGGCGGAAAGCGAGCTGCTGCAGGGGACGCTGGAGCCGACCAACG
AGCCGTACGCCATCGCCAAGATCGCCGGGATTAAACTGTGCGAGTCCTACAACCGGCAGTACGGTCGCGACTACCGTTCG
GTGATGCCAACCAACCTGTACGGCCCGCATGACAATTTCCACCCGGACAATTCACATGTGATCCCGGCGCTGCTGCGTCG
CTTTCATGAGGCTGCGCAGAGCCACGCGCCGGAGGTGGTGGTGTGGGGCAGCGGCACGCCGATGCGTGAATTTCTGCACG
TTGACGATATGGCGGCGGCCAGTATTCACGTGATGGAGCTGGCGCGTGAAGTGTGGCAGGAGAACACTGCCCCGATGCTG
TCGCACATTAACGTCGGCACTGGCGTGGACTGCACCATCCGCGAGCTGGCGCAGACCATCGCAAAGGTGGTGGGTTACCA
GGGCCGGGTGGTGTTCGACGCCGCGAAGCCGGACGGCACGCCGCGTAAATTGCTCGACGTCACGCGCCTGCATCAGCTTG
GCTGGTATCACGAAATTTCACTGGAGGCAGGGCTTGCCGGTACTTACCAGTGGTTCCTTGAGAATCAGCAACGGTTCCGG
GGGTGA

Upstream 100 bases:

>100_bases
TGGTCTCCGAGATGGTGGCGAACGATCTGGAGGCCGCGAAAAAACACTCACTGCTGAAATCTCACGGTTACGAGGTGGCC
ATCGCGCTGGAGTCCTGAGA

Downstream 100 bases:

>100_bases
CAATGTTTTTACGTCAGGAAGATTTCGCCGCCGTGGTGCGGGCGACGCCCCTCATCTCCCTCGATTTCATTGTGGAAAAC
GGCCAGGGGGAAATTTTACT

Product: GDP-L-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDGRAVQAFFAGAGIDQVYLAAAKVGGIVANNTYPADFI
YENMMIESNIIHAAHLHNVNKLLFLGSSCIYPKLARQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAASIHVMELAREVWQENTAPML
SHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAKPDGTPRKLLDVTRLHQLGWYHEISLEAGLAGTYQWFLENQQRFR
G

Sequences:

>Translated_321_residues
MNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDGRAVQAFFAGAGIDQVYLAAAKVGGIVANNTYPADFI
YENMMIESNIIHAAHLHNVNKLLFLGSSCIYPKLARQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAASIHVMELAREVWQENTAPML
SHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAKPDGTPRKLLDVTRLHQLGWYHEISLEAGLAGTYQWFLENQQRFR
G
>Mature_321_residues
MNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDGRAVQAFFAGAGIDQVYLAAAKVGGIVANNTYPADFI
YENMMIESNIIHAAHLHNVNKLLFLGSSCIYPKLARQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAASIHVMELAREVWQENTAPML
SHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAKPDGTPRKLLDVTRLHQLGWYHEISLEAGLAGTYQWFLENQQRFR
G

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=320, Percent_Identity=30.3125, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI7657641, Length=291, Percent_Identity=24.0549828178694, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788365, Length=321, Percent_Identity=90.6542056074766, Blast_Score=613, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17554428, Length=321, Percent_Identity=30.8411214953271, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982035, Length=356, Percent_Identity=23.5955056179775, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=358, Percent_Identity=23.463687150838, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922778, Length=326, Percent_Identity=31.2883435582822, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=26.6081871345029, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35815; Mature: 35815

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDGRAVQAFFAGAGIDQVY
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHCCHHHHHHHHCCCHHHHH
LAAAKVGGIVANNTYPADFIYENMMIESNIIHAAHLHNVNKLLFLGSSCIYPKLARQPMA
HHHHHHCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCHHEEEECCCCCCHHHHCCCCC
ESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGPHDNFHPDNSHV
HHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCH
IPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAASIHVMELAREVWQENTAPML
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAKPDGTPRKLLDVTRLHQLGWYHEIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEAGLAGTYQWFLENQQRFRG
ECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKQRIFVAGHRGMVGSAIVRQLAQRGDVELVLRTRDELDLLDGRAVQAFFAGAGIDQVY
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHCCHHHHHHHHCCCHHHHH
LAAAKVGGIVANNTYPADFIYENMMIESNIIHAAHLHNVNKLLFLGSSCIYPKLARQPMA
HHHHHHCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCHHEEEECCCCCCHHHHCCCCC
ESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGPHDNFHPDNSHV
HHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCH
IPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAASIHVMELAREVWQENTAPML
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SHINVGTGVDCTIRELAQTIAKVVGYQGRVVFDAAKPDGTPRKLLDVTRLHQLGWYHEIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEAGLAGTYQWFLENQQRFRG
ECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]