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The map label for this gene is nemA [H]

Identifier: 194734365

GI number: 194734365

Start: 1476841

End: 1477938

Strand: Reverse

Name: nemA [H]

Synonym: SeSA_A1533

Alternate gene names: 194734365

Gene position: 1477938-1476841 (Counterclockwise)

Preceding gene: 194737438

Following gene: 194737858

Centisome position: 31.38

GC content: 57.56

Gene sequence:

>1098_bases
ATGTCATCAGCAAAACTGTTTACCCCCCTCAAGGTGGGTGCCATCACGGCGACAAATCGCGTATTTATGGCGCCTTTGAC
GCGTTTGCGGAGTATTGAACCGGGCGACATTCCTACGCCGTTAATGGCGGAATATTACCGTCAACGCGCCAGCGCCGGAT
TGATCATCAGCGAAGCCACACAAATCTCCGCACAGGCGAAAGGCTATGCCGGCGCACCGGGATTACACAGCGATGAGCAG
ATCGCCGCCTGGAAAAAAATCACTCAGGGAGTTCATGCTCAGGGCGGCCATATGGCGGTGCAACTGTGGCATACGGGCCG
TATTTCACACGCCAGCCTGCAACCAGGCGGACAAGCGCCCGTCGCGCCATCCGCTATCAATGCGGGAACCCGCACCTCTT
TGCGTGACGAAAACGGCCAGGCCATCCGCGTAGAGACTTCCACGCCGCGCGCGCTGGATACTCACGAGATCCCCGGGATC
GTCAACGATTTCCGCCAGGCTATCGCCAATGCCCGAGAGGCAGGCTTCGAACTGGTGGAGCTGCACTCCGCGCACGGTTA
TCTGCTGCACCAGTTCCTCTCACCGTCTTCTAATCACCGTACCGATCAGTACGGCGGCAGCGTAGAGAATCGCGCCCGTC
TGGTGCTGGAAGTGGTTGATGCCGGCATTAAGGAATGGGGCGCGGACCGTATCGGCATCCGCCTTTCTCCTGTCGGCACT
TTCCAGAATGTCGATAACGGCCCGAATGAAGAGGCTGACGCCCTTTATCTGATTGAAGCGTTAGGCAAACGCGGCATTGC
TTATCTGCATATGTCTGAACCAGACTGGGCGGGCGGCGAACCGTACAGCGACGCGTTCCGTGAAAAAGTGCGCGCACGTT
TCCACGGTCCCATCATCGGCGCTGGCGCCTATACGCCAGAAAAAGCGGAAGATCTGATCGAAAAAGGGCTGATTGACGCC
GTGGCGTTTGGTCGCGATTACATCGCTAACCCGGATCTGGTAGCGCGTCTGCAATGTAAAGCGAAACTCAATCCACAGCG
TCCCGAAAGTTTTTATGGCGGCGGCGCGGAAGGCTACACTGACTACCCTACTCTGTAA

Upstream 100 bases:

>100_bases
TTGGCGCATGTTAAGACCATCATTGCAACGCCTGAGCTGTAACAGGCGTTTTTATTTCCTTTATTACAAGACGACCGGTC
TACTTATATCAGGAGCCACT

Downstream 100 bases:

>100_bases
TTTCATTGATAGCGGCCTCACATCGCCGCTATACTTTGAATATCTTGACTATTTTAAGGACAACGAAAATGCGTTTATTG
CATACTATGCTGCGCGTCGG

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQ
IAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGI
VNDFRQAIANAREAGFELVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDA
VAFGRDYIANPDLVARLQCKAKLNPQRPESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQ
IAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGI
VNDFRQAIANAREAGFELVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDA
VAFGRDYIANPDLVARLQCKAKLNPQRPESFYGGGAEGYTDYPTL
>Mature_364_residues
SSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQI
AAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIV
NDFRQAIANAREAGFELVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGTF
QNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDAV
AFGRDYIANPDLVARLQCKAKLNPQRPESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=91.7808219178082, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=32.1937321937322, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=28.0453257790368, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=351, Percent_Identity=28.4900284900285, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=353, Percent_Identity=28.6118980169972, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=352, Percent_Identity=28.4090909090909, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=353, Percent_Identity=26.9121813031161, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=273, Percent_Identity=29.3040293040293, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=270, Percent_Identity=27.4074074074074, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=386, Percent_Identity=39.6373056994819, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=382, Percent_Identity=37.17277486911, Blast_Score=219, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39442; Mature: 39311

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCCCCCCEEECEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGLHSDEQIAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIVNDFRQAIANAREAGFELVE
CCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIG
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTPEKAEDLIEKGLIDAVAFGRDYIANPDLVARLQCKAKLNPQRPESFYGGGAEGYT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCCCCCEEECEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGLHSDEQIAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIVNDFRQAIANAREAGFELVE
CCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIG
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTPEKAEDLIEKGLIDAVAFGRDYIANPDLVARLQCKAKLNPQRPESFYGGGAEGYT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]