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The map label for this gene is ligB [H]

Identifier: 194734322

GI number: 194734322

Start: 3816318

End: 3818003

Strand: Reverse

Name: ligB [H]

Synonym: SeSA_A3947

Alternate gene names: 194734322

Gene position: 3818003-3816318 (Counterclockwise)

Preceding gene: 194733970

Following gene: 194735115

Centisome position: 81.08

GC content: 56.35

Gene sequence:

>1686_bases
ATGAGATTATGGAAAAGTATGGCGTGGGGAATTTTATTGTGGCATTCGCAGAGTGGGGCGCTTTGCCCGGCCTGGCCGCC
AGCAAGGGCCGCTGAAGAAATCGCCCGTTTACAGCAACAGCTCGCTGACTGGAATGACATTTACTGGAAACAAGGCGTTA
GCGCGGTTGACGATAGTGTGTACGACCAACTCAGCGCAAGGCTGGTTCAGTGGCAACGCTGTGTTGGTCAGGATGTTTCA
TCTACTCCGGTTTCACCGCCGTTAAACGGTACAACAATGCATCCTGTTGCGCATACGGGCGTACGTAAACTCGCGGACCG
GCAGGCGGTAGAACAGTGGATGCGCGGGCGCAGCGAGCTTTGGGTACAACCAAAAGTGGATGGCGTAGCGGTAACGCTGG
TTTATCAAAACGGTAAACTGGCCAGGGCTATCAGCCGAGGTAACGGACTACAAGGCGAGGACTGGACGCCAAAAATTCGC
CTGATCCCCTCTATACCGCAGACAACACAAGGCGCGCTTGCTAACGCGGTGTTGCAGGGCGAAATCTTTCTACAGCGCGA
GGGGCATATCCAGCAACGGATGGGCGGGATGAATGCGCGCTCGAAAGTCGCAGGAATGTTAATGCGCCAGGATAACGCCT
CCGCGCTAAATTCGTTGGGTATTTTTATTTGGGCGTGGCCGGACGGTCCGGCAAATATGCCCGAAAGATTAAGCCAACTT
GCTAAGGCTGGATTCAGTCTGACGAAGAAATATACCCTGGCGGTAAAAGATGCCAGCGAGGTCGAGCGGGCGCGCCAGTC
ATGGCTGACGTCAGCGTTGCCCTTTGTGACGGATGGCGTAGTGATACGCATGGCTAAAGAGCCCGCGGCACAATACTGGC
GACCCGGGCAGGGCGACTGGCTGGCAGCATGGAAATATCCGCCAGTAGCGCAGGTCGCGCAAGTGAGCGCTATTCAGTTC
TCGGTGGGGAAAAGCGGCAAAATTACCGTAGTAGCGTCGCTTGTCCCCGTTATATTGGATGATAAGCGGGTTCAGCGGGT
CAATATCGGCTCTGTGAAACGTTGGGAAGCGTGGGATATCGCGCCGGGCGATCAGATCCTGGTGAGTCTGGCCGGGCAAG
GCATTCCCCGGCTTGATGAGGTCGTCTGGCGGAGTCGTGAGCGGAGTAAGCCTGTACCGCCTGATAGCCATTTCAACTCG
CTGACCTGCTTTTACGCGTCGGCAACGTGTCAGGAACAGTTTATCTCCAGGCTGGTATGGCTGGGATCACGATCGGCGTT
GGGTCTGGACGGAATGGGCGAAGCCAGCTGGCGGGCCTTGCATCAGACGCATCGCTTTGAGCATATCTTCTCCTGGCTTG
CCCTGACGTCAGCGCAAATAGCCAACACGCCAGGCTTTGCTAAAGGAAAAAGCGAGCAGATATGGCGGCAATTTAACCTG
GCGCGCCGGCAGTCGTTTACCCGCTGGATCATGGCGATGGATATCCCCTTAACGCAGGCCGCATTACAGGCCAGCGGCGA
TCGTTCATGGGAGCAGTTATTAATGCGAACAGAGCAACACTGGCGGCAGTTGCCAGCGACGGGCGAGCGCCGTGCCGGGA
GAGTTATCGACTGGCGGGATAATCCGCAGATCAAAGCGCTGAGCAGATGGCTGTCTGCTCAGCATATTCCCGGATTTGGG
TCTTAG

Upstream 100 bases:

>100_bases
CCAAAAAATCTGGAAAGCGGCTCGGATGATGCATTTTTACCTGTCGGTTTTTATTCACTAAAGCGCTATACCTGCCGGGC
AATGTGCGCAGGAGGTCGTG

Downstream 100 bases:

>100_bases
TGCCCTTCATGGCGGTAATAAAATACCGGCAGGCCAAGTTTAAGACGTAGCGCTAATAACCTTGCTGTAAAGCCAAACAG
TAACGTAGAGATAACGACGA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRLWKSMAWGILLWHSQSGALCPAWPPARAAEEIARLQQQLADWNDIYWKQGVSAVDDSVYDQLSARLVQWQRCVGQDVS
STPVSPPLNGTTMHPVAHTGVRKLADRQAVEQWMRGRSELWVQPKVDGVAVTLVYQNGKLARAISRGNGLQGEDWTPKIR
LIPSIPQTTQGALANAVLQGEIFLQREGHIQQRMGGMNARSKVAGMLMRQDNASALNSLGIFIWAWPDGPANMPERLSQL
AKAGFSLTKKYTLAVKDASEVERARQSWLTSALPFVTDGVVIRMAKEPAAQYWRPGQGDWLAAWKYPPVAQVAQVSAIQF
SVGKSGKITVVASLVPVILDDKRVQRVNIGSVKRWEAWDIAPGDQILVSLAGQGIPRLDEVVWRSRERSKPVPPDSHFNS
LTCFYASATCQEQFISRLVWLGSRSALGLDGMGEASWRALHQTHRFEHIFSWLALTSAQIANTPGFAKGKSEQIWRQFNL
ARRQSFTRWIMAMDIPLTQAALQASGDRSWEQLLMRTEQHWRQLPATGERRAGRVIDWRDNPQIKALSRWLSAQHIPGFG
S

Sequences:

>Translated_561_residues
MRLWKSMAWGILLWHSQSGALCPAWPPARAAEEIARLQQQLADWNDIYWKQGVSAVDDSVYDQLSARLVQWQRCVGQDVS
STPVSPPLNGTTMHPVAHTGVRKLADRQAVEQWMRGRSELWVQPKVDGVAVTLVYQNGKLARAISRGNGLQGEDWTPKIR
LIPSIPQTTQGALANAVLQGEIFLQREGHIQQRMGGMNARSKVAGMLMRQDNASALNSLGIFIWAWPDGPANMPERLSQL
AKAGFSLTKKYTLAVKDASEVERARQSWLTSALPFVTDGVVIRMAKEPAAQYWRPGQGDWLAAWKYPPVAQVAQVSAIQF
SVGKSGKITVVASLVPVILDDKRVQRVNIGSVKRWEAWDIAPGDQILVSLAGQGIPRLDEVVWRSRERSKPVPPDSHFNS
LTCFYASATCQEQFISRLVWLGSRSALGLDGMGEASWRALHQTHRFEHIFSWLALTSAQIANTPGFAKGKSEQIWRQFNL
ARRQSFTRWIMAMDIPLTQAALQASGDRSWEQLLMRTEQHWRQLPATGERRAGRVIDWRDNPQIKALSRWLSAQHIPGFG
S
>Mature_561_residues
MRLWKSMAWGILLWHSQSGALCPAWPPARAAEEIARLQQQLADWNDIYWKQGVSAVDDSVYDQLSARLVQWQRCVGQDVS
STPVSPPLNGTTMHPVAHTGVRKLADRQAVEQWMRGRSELWVQPKVDGVAVTLVYQNGKLARAISRGNGLQGEDWTPKIR
LIPSIPQTTQGALANAVLQGEIFLQREGHIQQRMGGMNARSKVAGMLMRQDNASALNSLGIFIWAWPDGPANMPERLSQL
AKAGFSLTKKYTLAVKDASEVERARQSWLTSALPFVTDGVVIRMAKEPAAQYWRPGQGDWLAAWKYPPVAQVAQVSAIQF
SVGKSGKITVVASLVPVILDDKRVQRVNIGSVKRWEAWDIAPGDQILVSLAGQGIPRLDEVVWRSRERSKPVPPDSHFNS
LTCFYASATCQEQFISRLVWLGSRSALGLDGMGEASWRALHQTHRFEHIFSWLALTSAQIANTPGFAKGKSEQIWRQFNL
ARRQSFTRWIMAMDIPLTQAALQASGDRSWEQLLMRTEQHWRQLPATGERRAGRVIDWRDNPQIKALSRWLSAQHIPGFG
S

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=559, Percent_Identity=68.8729874776386, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=519, Percent_Identity=25.0481695568401, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 62795; Mature: 62795

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLWKSMAWGILLWHSQSGALCPAWPPARAAEEIARLQQQLADWNDIYWKQGVSAVDDSV
CCHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
YDQLSARLVQWQRCVGQDVSSTPVSPPLNGTTMHPVAHTGVRKLADRQAVEQWMRGRSEL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHE
WVQPKVDGVAVTLVYQNGKLARAISRGNGLQGEDWTPKIRLIPSIPQTTQGALANAVLQG
EECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
EIFLQREGHIQQRMGGMNARSKVAGMLMRQDNASALNSLGIFIWAWPDGPANMPERLSQL
EEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHH
AKAGFSLTKKYTLAVKDASEVERARQSWLTSALPFVTDGVVIRMAKEPAAQYWRPGQGDW
HHCCCHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCE
LAAWKYPPVAQVAQVSAIQFSVGKSGKITVVASLVPVILDDKRVQRVNIGSVKRWEAWDI
EEEECCCCHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
APGDQILVSLAGQGIPRLDEVVWRSRERSKPVPPDSHFNSLTCFYASATCQEQFISRLVW
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
LGSRSALGLDGMGEASWRALHQTHRFEHIFSWLALTSAQIANTPGFAKGKSEQIWRQFNL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ARRQSFTRWIMAMDIPLTQAALQASGDRSWEQLLMRTEQHWRQLPATGERRAGRVIDWRD
HHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
NPQIKALSRWLSAQHIPGFGS
CCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLWKSMAWGILLWHSQSGALCPAWPPARAAEEIARLQQQLADWNDIYWKQGVSAVDDSV
CCHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
YDQLSARLVQWQRCVGQDVSSTPVSPPLNGTTMHPVAHTGVRKLADRQAVEQWMRGRSEL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCHHE
WVQPKVDGVAVTLVYQNGKLARAISRGNGLQGEDWTPKIRLIPSIPQTTQGALANAVLQG
EECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
EIFLQREGHIQQRMGGMNARSKVAGMLMRQDNASALNSLGIFIWAWPDGPANMPERLSQL
EEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHH
AKAGFSLTKKYTLAVKDASEVERARQSWLTSALPFVTDGVVIRMAKEPAAQYWRPGQGDW
HHCCCHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCE
LAAWKYPPVAQVAQVSAIQFSVGKSGKITVVASLVPVILDDKRVQRVNIGSVKRWEAWDI
EEEECCCCHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
APGDQILVSLAGQGIPRLDEVVWRSRERSKPVPPDSHFNSLTCFYASATCQEQFISRLVW
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
LGSRSALGLDGMGEASWRALHQTHRFEHIFSWLALTSAQIANTPGFAKGKSEQIWRQFNL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ARRQSFTRWIMAMDIPLTQAALQASGDRSWEQLLMRTEQHWRQLPATGERRAGRVIDWRD
HHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
NPQIKALSRWLSAQHIPGFGS
CCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA