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The map label for this gene is rfbI [H]

Identifier: 194734228

GI number: 194734228

Start: 2195681

End: 2196655

Strand: Reverse

Name: rfbI [H]

Synonym: SeSA_A2326

Alternate gene names: 194734228

Gene position: 2196655-2195681 (Counterclockwise)

Preceding gene: 194734639

Following gene: 194737866

Centisome position: 46.65

GC content: 40.72

Gene sequence:

>975_bases
GTGTCTCATATTATTAAGATTTTTCCATCAAATATTGAATTTTCCGGTAGAGAGGATGAATCAATCCTCGATGCTGCGCT
ATCGGCTGGCATCCATCTTGAACATAGCTGCAAAGCGGGTGATTGTGGTATCTGTGAGTCCGATTTGTTAGCGGGAGAAG
TTGTTGACTCCAAAGGTAATATTTTTGGACAGGGTGATAAAATACTAACCTGCTGCTGTAAACCTAAAACCGCCCTTGAG
CTAAATGCACATTTTTTTCCTGAACTAGCTGGACAGACAAAAAAAATTGTCCCATGCAAGGTAAATAGTGCTGTACTGGT
TTCAGGCGATGTTATGACTTTGAAGTTACGTACACCACCAACAGCAAAAATTGGCTTCCTTCCAGGGCAGTATATCAATT
TACATTATAAAGGTGTAACTCGCAGTTATTCTATCGCTAATAGTGATGAGTCGAATGGTATTGAGTTGCATGTAAGGAAT
GTTCCCAATGGTCAGATGAGCTCGCTCATTTTTGGGGAGTTACAAGAAAATACTCTTATGCGCATTGAAGGGCCTTGCGG
AACATTTTTTATTCGTGAAAGTGACAGACCTATAATCTTCCTTGCAGGCGGTACTGGATTCGCTCCAGTTAAATCAATGG
TTGAGCATCTCATTCAGGGAAAATGTCGTCGTGAGATCTACATTTACTGGGGAATGCAAGATAGTAAAGATTTTTACTCT
GAATTACCGCAGCAGTGGAGTGAACAGCACGACAACGTTCATTATATCCCTGTTGTTTCTGGTGATGACGCCGAATGGGG
GGGAAGAAAGGGATTTGTCCATCATGCCGTGATGGATGATTTTGATTCTCTAGAGTTCTTCGATATATATGCATGTGGTT
CACCTGTGATGATCGATGCCAGTAAAAAGGACTTTATGATGAAAAATCTCTCTGTAGAACATTTCTATTCTGATGCATTT
ACCGCATCTAAATAA

Upstream 100 bases:

>100_bases
CAATAGGTATTGAATGGCCTTTTTCTCAGCTGCCTGAGCTTTCAGCAAAAGATGCTGCAGCACCTTTACTGCATCAAGCC
TTGTTAACAGAGTAAGCATC

Downstream 100 bases:

>100_bases
TATTGAGGATAATTTATGAAAGCGGTCATCCTGGCTGGTGGACTTGGTACCAGACTAAGTGAAGAAACAATTGTAAAACC
AAAACCGATGGTAGAAATTG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALE
LNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRN
VPNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQDSKDFYS
ELPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAF
TASK

Sequences:

>Translated_324_residues
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALE
LNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRN
VPNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQDSKDFYS
ELPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAF
TASK
>Mature_323_residues
SHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALEL
NAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNV
PNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQDSKDFYSE
LPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAFT
ASK

Specific function: Catalyzes The Reduction Of Soluble Flavins By Reduced Pyridine Nucleotides. Seems To Reduces The Complexed Fe(3+) Iron Of Siderophores To Fe(2+), Thus Releasing It From The Chelator. [C]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=230, Percent_Identity=29.5652173913043, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 35908; Mature: 35777

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGN
CCCEEEEECCCEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCC
IFGQGDKILTCCCKPKTALELNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPP
EEECCCEEEEEECCCCCEEEECCEECHHHCCCCCEEEEEECCCEEEEECCEEEEEECCCC
TAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNVPNGQMSSLIFGELQENTLM
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCHHHHHEEEECCCCEEE
RIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQDSKDFYS
EEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
ELPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDA
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCEEEEEEECCCCEEEEC
SKKDFMMKNLSVEHFYSDAFTASK
CCCHHHHHCCCHHHEECCHHCCCC
>Mature Secondary Structure 
SHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGN
CCEEEEECCCEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCC
IFGQGDKILTCCCKPKTALELNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPP
EEECCCEEEEEECCCCCEEEECCEECHHHCCCCCEEEEEECCCEEEEECCEEEEEECCCC
TAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNVPNGQMSSLIFGELQENTLM
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCHHHHHEEEECCCCEEE
RIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQDSKDFYS
EEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
ELPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDA
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCEEEEEEECCCCEEEEC
SKKDFMMKNLSVEHFYSDAFTASK
CCCHHHHHCCCHHHEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]