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The map label for this gene is hgiDIIM [H]

Identifier: 194335097

GI number: 194335097

Start: 2491044

End: 2492066

Strand: Reverse

Name: hgiDIIM [H]

Synonym: Paes_2314

Alternate gene names: 194335097

Gene position: 2492066-2491044 (Counterclockwise)

Preceding gene: 194335098

Following gene: 194335096

Centisome position: 99.17

GC content: 37.15

Gene sequence:

>1023_bases
ATGAAATTGATATCTATAGATATATTTAGTGGAGTTGGAGGGCTTACAGAAGGACTCCACAAGGCTGGTTTCCAGACAGA
ACTTGCTTTTGAAATTGACGAGCTTGCATCAAAGGTATATAAACTGAATCATAAAAAGACCAAAGTCATAACTGATGATA
TCAGAAACATCTCAACTGAAAATGTAAAGCAAGAACTAGGCAACAAAACGATTCATTTACTTGCTGGCTGCCCACCTTGT
CAGGGATTCAGTTCAATACGAAGACTTAATAAACCCGAGCCCGTCAAAGATGATAGAAACAAGCTAATCAAGGAATTTGT
AAGGTTTGTGAAAGAATTAATGCCCTATACCTTCATGATGGAAAACGTGCCGGGTTTGGCACTCGACAAATCCTTTAAAT
CTGCTCTTAAAGAATTGGAAAATGCAGGCTACTACAAGCCTGATTGGAAGATTGTTAACATTAAGGATTACGGTGTTCCT
CAAAGCAGAAAAAGACTCGTGTTGGTTGGTTCAAGATTAGCTCCCTTAAGAATTGCAGAACCTACAAAAAAGAAAAAGAC
AGTTAGACAAGTAATAGGCAAACTCCCAAAACCTGAGAACTCAATGGACCCATTACACCAGATTTTTCCTAGTCACTCAG
ATGAAATATTGAATTTGATTAAGGACATCCCCAAAAATGGTGGAAGTAGGAAAGATTTGGGGAAAGACCGACAATTAAAA
TGTCATCAAAAAGAAAATGTAGGTTTTAATGATGTATATGGTCGGTTAAGATGGGATGATTTTTCTACAACAATCACGGG
TGGTTGTTTAAATCCTTCTAAAGGTCGTTTTCTTCATCCAGAACAAGATAGATGTATTTCAGCAAGAGAAGCGTCTATGC
TTCAGTCATTCCCAAAGACTTATAAGTTTCCAATTGATGCTCCAAGAACAAAAATTGCATTGATGATTGGGAATGCTTTG
CCACCTGAGTTTAGTAAAATCCAAGCTGAGAACATTAAATCGCACATTTTAACTCACCTTTAA

Upstream 100 bases:

>100_bases
AAAATATAAACAATTGTGTTTATCTGTCCAATTTTGCGGTATAAGAACTATGAATATACATAGACGTTGAAAGCCATATT
AGAAAACCGATATTAGATAT

Downstream 100 bases:

>100_bases
CTAAAAAATTTTGAATGCCACTATTTAGAGACACTTATTCCGCTGCTGATTTATCATTAACTGATGATCAATATAATGCA
GTCATAGATGACACAAATGA

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKLISIDIFSGVGGLTEGLHKAGFQTELAFEIDELASKVYKLNHKKTKVITDDIRNISTENVKQELGNKTIHLLAGCPPC
QGFSSIRRLNKPEPVKDDRNKLIKEFVRFVKELMPYTFMMENVPGLALDKSFKSALKELENAGYYKPDWKIVNIKDYGVP
QSRKRLVLVGSRLAPLRIAEPTKKKKTVRQVIGKLPKPENSMDPLHQIFPSHSDEILNLIKDIPKNGGSRKDLGKDRQLK
CHQKENVGFNDVYGRLRWDDFSTTITGGCLNPSKGRFLHPEQDRCISAREASMLQSFPKTYKFPIDAPRTKIALMIGNAL
PPEFSKIQAENIKSHILTHL

Sequences:

>Translated_340_residues
MKLISIDIFSGVGGLTEGLHKAGFQTELAFEIDELASKVYKLNHKKTKVITDDIRNISTENVKQELGNKTIHLLAGCPPC
QGFSSIRRLNKPEPVKDDRNKLIKEFVRFVKELMPYTFMMENVPGLALDKSFKSALKELENAGYYKPDWKIVNIKDYGVP
QSRKRLVLVGSRLAPLRIAEPTKKKKTVRQVIGKLPKPENSMDPLHQIFPSHSDEILNLIKDIPKNGGSRKDLGKDRQLK
CHQKENVGFNDVYGRLRWDDFSTTITGGCLNPSKGRFLHPEQDRCISAREASMLQSFPKTYKFPIDAPRTKIALMIGNAL
PPEFSKIQAENIKSHILTHL
>Mature_340_residues
MKLISIDIFSGVGGLTEGLHKAGFQTELAFEIDELASKVYKLNHKKTKVITDDIRNISTENVKQELGNKTIHLLAGCPPC
QGFSSIRRLNKPEPVKDDRNKLIKEFVRFVKELMPYTFMMENVPGLALDKSFKSALKELENAGYYKPDWKIVNIKDYGVP
QSRKRLVLVGSRLAPLRIAEPTKKKKTVRQVIGKLPKPENSMDPLHQIFPSHSDEILNLIKDIPKNGGSRKDLGKDRQLK
CHQKENVGFNDVYGRLRWDDFSTTITGGCLNPSKGRFLHPEQDRCISAREASMLQSFPKTYKFPIDAPRTKIALMIGNAL
PPEFSKIQAENIKSHILTHL

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=184, Percent_Identity=32.6086956521739, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI195927037, Length=184, Percent_Identity=32.6086956521739, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI4758184, Length=200, Percent_Identity=24.5, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788271, Length=199, Percent_Identity=30.1507537688442, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38516; Mature: 38516

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISIDIFSGVGGLTEGLHKAGFQTELAFEIDELASKVYKLNHKKTKVITDDIRNISTE
CEEEEEEHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
NVKQELGNKTIHLLAGCPPCQGFSSIRRLNKPEPVKDDRNKLIKEFVRFVKELMPYTFMM
HHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
ENVPGLALDKSFKSALKELENAGYYKPDWKIVNIKDYGVPQSRKRLVLVGSRLAPLRIAE
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
PTKKKKTVRQVIGKLPKPENSMDPLHQIFPSHSDEILNLIKDIPKNGGSRKDLGKDRQLK
CHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
CHQKENVGFNDVYGRLRWDDFSTTITGGCLNPSKGRFLHPEQDRCISAREASMLQSFPKT
CHHHCCCCHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
YKFPIDAPRTKIALMIGNALPPEFSKIQAENIKSHILTHL
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLISIDIFSGVGGLTEGLHKAGFQTELAFEIDELASKVYKLNHKKTKVITDDIRNISTE
CEEEEEEHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
NVKQELGNKTIHLLAGCPPCQGFSSIRRLNKPEPVKDDRNKLIKEFVRFVKELMPYTFMM
HHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
ENVPGLALDKSFKSALKELENAGYYKPDWKIVNIKDYGVPQSRKRLVLVGSRLAPLRIAE
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
PTKKKKTVRQVIGKLPKPENSMDPLHQIFPSHSDEILNLIKDIPKNGGSRKDLGKDRQLK
CHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
CHQKENVGFNDVYGRLRWDDFSTTITGGCLNPSKGRFLHPEQDRCISAREASMLQSFPKT
CHHHCCCCHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
YKFPIDAPRTKIALMIGNALPPEFSKIQAENIKSHILTHL
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]