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The map label for this gene is murF [H]

Identifier: 194335055

GI number: 194335055

Start: 2445772

End: 2447178

Strand: Reverse

Name: murF [H]

Synonym: Paes_2267

Alternate gene names: 194335055

Gene position: 2447178-2445772 (Counterclockwise)

Preceding gene: 194335056

Following gene: 194335054

Centisome position: 97.38

GC content: 53.8

Gene sequence:

>1407_bases
ATGGGGATGTGTTTGACAAAGGATGATCTTGAATCGGTTGGCACTCCTGCCGGCTTTGCTCTGGGTGAAGCGGTGGAGTG
GTGTGATCCATCTGTTTCAATCGATTCAAGAGATATCAGGAGCGGCAGTATTTTCTTTGCTCTGAAGGGAGAGCGGACAG
ATGGTCATGAGTATGTCTCCCTCGCGTTCAAAAGCGGGGCGCGTTGTGCTGTGGTCAACAGGGAGTGGTACAGCAGGCAT
GCTGAATCCGTTCATCAGGAGGGTTGGCGATATCTTGTGGTGCACGATACCGTTCGGGCCCTGCAGCAGCTTGCCTCGAT
CTATCGGAGCAAGTTCAGCATTCCTGTTATCGGTATCGGCGGCAGTAATGGTAAAACGACAACCAAGGAGATGACCGCAG
CCGTTCTCGGGACGACATACAAGGTGCATATGAGCAAGGGAAATCTCAATAACCATCTCGGTGTGCCGCTCACGCTTTTC
GGTTTACGCGATACCCATGATCTTGCGGTTGTCGAAATGGGAATCAATCATCCCGGAGAGATGGAACTGCTCACCTCCAT
TGCCCGTCCCGACTTCGCTCTCCTGACAAATATCGGTCATGAACATCTCGAGTTTCTTGGCGATCTGGATGGAGTGGCTG
AGGCTGAGACGGTGCTTTACCATGATACCATCATGCGTGGCGGAACGGTTTTTGTCAATACCGGCGACGCACGGCTTCTT
GCTGCGGCTCGCGAGATCCAAAATCGTGTTGACTACGGTCCTGAGCGGGACGGGAACCTGATCTGGCCGGAGAAGATTGT
GCTTGATCCCGCTGGCAGGACGACCTTTACTCTTTGCACTGCTTCCGGAAGCGTCAGCCTGATGCTTCATTTTTCCGGAC
GTCACAATGTTGATAACGCCGTTGCTGCCGCTGCTGTCGGGAGCTGTTTCAATGTTCCTCTGAACGCTATCGCAGAAGGT
CTCGGGTCGCTTGTGCCTGCCGATGGGTGGAAACGGCTTGAGTTTATTGAGGCTGGCGGGATAAGGATTTTCAACGATAC
CTATAACGCCAATCCCGATTCGGTTACGATGGCGCTGAAAACCGTTTGCGAGATCCCCTGTTCCGGCCGTAGGGTCCTGG
TCTTTGCCGATATGCTTGAACTTGGAGAGGTTTCAGATGCCGAGCACCGCCGGATGGGAGCGCTTGTTGCTCAGTTGCCG
TTCGACGCTGTCTATACCTATGGAGAGCATGCGGCATTGAGCTGTCTCTCTGCCGGACCGAAGTGCAGGGGTCATTATAC
CAGTCACGCGCATTTGCTCGATGCACTGAAGGCGTATCTCTCTCAGGACGACCTTCTCCTTCTTAAAGGGTCGAGAGGGA
TGAGGCTCGAACTGATAGCTGAAGGCCTTCGAGCCACCGACAAGTAG

Upstream 100 bases:

>100_bases
GGGATGAGGATTTCGAGCACCGACCAACGACGTATTCGGGCCGCGCAACAAATTTATAGAGGAGCCCTGGAGTAACCGAG
TAAATGAAAGGTGTATTTTC

Downstream 100 bases:

>100_bases
AAAGAATTATTTTTTCAGAAAATCTCTATCATGCTCTATTACCTGTTACAGTATATCAACGACGTTTTTGATCCACCGGG
TTTCGGTGTTGTCGAATATA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGMCLTKDDLESVGTPAGFALGEAVEWCDPSVSIDSRDIRSGSIFFALKGERTDGHEYVSLAFKSGARCAVVNREWYSRH
AESVHQEGWRYLVVHDTVRALQQLASIYRSKFSIPVIGIGGSNGKTTTKEMTAAVLGTTYKVHMSKGNLNNHLGVPLTLF
GLRDTHDLAVVEMGINHPGEMELLTSIARPDFALLTNIGHEHLEFLGDLDGVAEAETVLYHDTIMRGGTVFVNTGDARLL
AAAREIQNRVDYGPERDGNLIWPEKIVLDPAGRTTFTLCTASGSVSLMLHFSGRHNVDNAVAAAAVGSCFNVPLNAIAEG
LGSLVPADGWKRLEFIEAGGIRIFNDTYNANPDSVTMALKTVCEIPCSGRRVLVFADMLELGEVSDAEHRRMGALVAQLP
FDAVYTYGEHAALSCLSAGPKCRGHYTSHAHLLDALKAYLSQDDLLLLKGSRGMRLELIAEGLRATDK

Sequences:

>Translated_468_residues
MGMCLTKDDLESVGTPAGFALGEAVEWCDPSVSIDSRDIRSGSIFFALKGERTDGHEYVSLAFKSGARCAVVNREWYSRH
AESVHQEGWRYLVVHDTVRALQQLASIYRSKFSIPVIGIGGSNGKTTTKEMTAAVLGTTYKVHMSKGNLNNHLGVPLTLF
GLRDTHDLAVVEMGINHPGEMELLTSIARPDFALLTNIGHEHLEFLGDLDGVAEAETVLYHDTIMRGGTVFVNTGDARLL
AAAREIQNRVDYGPERDGNLIWPEKIVLDPAGRTTFTLCTASGSVSLMLHFSGRHNVDNAVAAAAVGSCFNVPLNAIAEG
LGSLVPADGWKRLEFIEAGGIRIFNDTYNANPDSVTMALKTVCEIPCSGRRVLVFADMLELGEVSDAEHRRMGALVAQLP
FDAVYTYGEHAALSCLSAGPKCRGHYTSHAHLLDALKAYLSQDDLLLLKGSRGMRLELIAEGLRATDK
>Mature_467_residues
GMCLTKDDLESVGTPAGFALGEAVEWCDPSVSIDSRDIRSGSIFFALKGERTDGHEYVSLAFKSGARCAVVNREWYSRHA
ESVHQEGWRYLVVHDTVRALQQLASIYRSKFSIPVIGIGGSNGKTTTKEMTAAVLGTTYKVHMSKGNLNNHLGVPLTLFG
LRDTHDLAVVEMGINHPGEMELLTSIARPDFALLTNIGHEHLEFLGDLDGVAEAETVLYHDTIMRGGTVFVNTGDARLLA
AAREIQNRVDYGPERDGNLIWPEKIVLDPAGRTTFTLCTASGSVSLMLHFSGRHNVDNAVAAAAVGSCFNVPLNAIAEGL
GSLVPADGWKRLEFIEAGGIRIFNDTYNANPDSVTMALKTVCEIPCSGRRVLVFADMLELGEVSDAEHRRMGALVAQLPF
DAVYTYGEHAALSCLSAGPKCRGHYTSHAHLLDALKAYLSQDDLLLLKGSRGMRLELIAEGLRATDK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=439, Percent_Identity=34.3963553530752, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50743; Mature: 50612

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMCLTKDDLESVGTPAGFALGEAVEWCDPSVSIDSRDIRSGSIFFALKGERTDGHEYVS
CCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEE
LAFKSGARCAVVNREWYSRHAESVHQEGWRYLVVHDTVRALQQLASIYRSKFSIPVIGIG
EEECCCCEEEEECHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEC
GSNGKTTTKEMTAAVLGTTYKVHMSKGNLNNHLGVPLTLFGLRDTHDLAVVEMGINHPGE
CCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCH
MELLTSIARPDFALLTNIGHEHLEFLGDLDGVAEAETVLYHDTIMRGGTVFVNTGDARLL
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCHHHHHEEEEHHHHCCCEEEEECCCHHHH
AAAREIQNRVDYGPERDGNLIWPEKIVLDPAGRTTFTLCTASGSVSLMLHFSGRHNVDNA
HHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCHHH
VAAAAVGSCFNVPLNAIAEGLGSLVPADGWKRLEFIEAGGIRIFNDTYNANPDSVTMALK
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHH
TVCEIPCSGRRVLVFADMLELGEVSDAEHRRMGALVAQLPFDAVYTYGEHAALSCLSAGP
HHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCC
KCRGHYTSHAHLLDALKAYLSQDDLLLLKGSRGMRLELIAEGLRATDK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GMCLTKDDLESVGTPAGFALGEAVEWCDPSVSIDSRDIRSGSIFFALKGERTDGHEYVS
CCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEE
LAFKSGARCAVVNREWYSRHAESVHQEGWRYLVVHDTVRALQQLASIYRSKFSIPVIGIG
EEECCCCEEEEECHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEC
GSNGKTTTKEMTAAVLGTTYKVHMSKGNLNNHLGVPLTLFGLRDTHDLAVVEMGINHPGE
CCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCH
MELLTSIARPDFALLTNIGHEHLEFLGDLDGVAEAETVLYHDTIMRGGTVFVNTGDARLL
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCHHHHHEEEEHHHHCCCEEEEECCCHHHH
AAAREIQNRVDYGPERDGNLIWPEKIVLDPAGRTTFTLCTASGSVSLMLHFSGRHNVDNA
HHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCHHH
VAAAAVGSCFNVPLNAIAEGLGSLVPADGWKRLEFIEAGGIRIFNDTYNANPDSVTMALK
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHH
TVCEIPCSGRRVLVFADMLELGEVSDAEHRRMGALVAQLPFDAVYTYGEHAALSCLSAGP
HHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCC
KCRGHYTSHAHLLDALKAYLSQDDLLLLKGSRGMRLELIAEGLRATDK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]