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The map label for this gene is phoA [H]

Identifier: 194335014

GI number: 194335014

Start: 2405262

End: 2406707

Strand: Reverse

Name: phoA [H]

Synonym: Paes_2225

Alternate gene names: 194335014

Gene position: 2406707-2405262 (Counterclockwise)

Preceding gene: 194335015

Following gene: 194335013

Centisome position: 95.77

GC content: 56.78

Gene sequence:

>1446_bases
ATGAGAAGACCTGGCAGGGCGGCTTATCTTCTGTTTCTCCTGTTCATGATCAGCGCTTTGTCCGCCTGCCGCTCAACGGC
TGATGGCAGGCAGCACCAGCCAGATCAACAGCAATCGTTTCCCCGCTACATCTTTTACTTTATCGGAGACGGTATGGGAC
TGGCCCAGGTTGCGCTCGGCGAAGCGCTTGCTGGAGAGCAAGGGGGGTTAGCCATGCTCAGGATGCCGGTAACGGGACTG
ATGACGACGTACGCTCTCGACCGCTCTATTACGGATTCTGCGGCAGCAGGGACAGCTATGGCAACAGGCTATAAAACGAC
GGTAGGCACCATTGCCCGTAATGACCGTCACAGTGCTGATCTGACAACAATTGCTGAAGCGGCCAGGGATCACGGCTTCG
GAGTCGGGATTGTCAGCAGCGTCAGTATCGATCACGCAACGCCAGCCTGTTTTTATGCCCATGCAGACAGCAGAAATGAG
TATGGGGCAATTGCCCGGCAGATGGCGCAGAGCGGTTTCGATTACTTCGGCGGCGGCTACGCCCTGTCGGCGTTACGTGA
CGACACGCTTGACGATGAGCTTCGCCGTCTCATGTTGCAGAAGGGCTACACGATTGCCGCCGGAAAAGAGGCTATCGAGG
CTGCCTTGCCCGGATCGCGATGCTGGGCGGTCGGGGCGGTTGACTCGAAAGGCGCGCTGGCGTATGAGATTGACCGCAAT
GAGGAAGACGCTGATCTGGCCTTTTTTACCCGCTACGGTATCCGGCTGCTTGATCATCACCGGGGTTTTTTTCTGATGGT
CGAGGGTGGCAAAATCGACTGGGCATGCCATGCCAATGACGGAGCAACCGTGTCGAGAGAGGTTAAGGCGTTTGACCGCG
CTATCGGTGAAGCGCTGGCGTTTTACCGGAGCCATCCGGATGAGACCCTGATTATTGTGACCGCAGACCATGAATGCGGC
GGTCTTGCCCTCGGCAACAATCAGGTCCGCTATAGCGGTCGGCCCTCTTTGCTCGACTATCAGCAGCTTTCGCTCGAGCG
GTTTTCAGCGAAAGTCAGAGCATGGTCAGGGAGCGGCAGGGTATCGTTTCCCATGGCGATGGACAGTCTCAGGGTGTATT
TCGGTCTTGGCAACATTGATCTGGATCCCCTGCTCGCACTTTCGCCCCCGGACAGCATCGATCTGGCAAAGGCCTACCAG
GCGTGCATGACGAGCAGTGAAGAACTCAATACCTACGGACGTAGCGATCCTTTTACTTCTTCTGCGGGTGCGCTTCTCAA
CCGTAAGGCCGGGATTGGCTGGACAACCGGTCACCATACCTCGGTTCCTGTTCCGGTTTTTGCGCTTGGCCGGGGACAGG
AACGCTTTGGAGGAAGCTATGATAATACCGAACTCGCCAAGAAGATGATGGATCTTTGCGGGTTGAGCCCTGTCGCCAAG
GAGTGA

Upstream 100 bases:

>100_bases
TTTTACCTGCTTTCGGATCGGAGACCTTCCTCCGTGAGCAGGTAAGGGTTTCGTCGTTATCGGACCCATTGAAATATGTT
CATCCTCAATCAACACTCCT

Downstream 100 bases:

>100_bases
AGTTTCTCCTTCCCTGAAGGGCTTTTTTTTTGTGTTTGTAAAAACAAAGTATTATTCTTTAAGAGAGCATTTGGATCGCA
TAATCTTTCTCTTCTCCCCA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRRPGRAAYLLFLLFMISALSACRSTADGRQHQPDQQQSFPRYIFYFIGDGMGLAQVALGEALAGEQGGLAMLRMPVTGL
MTTYALDRSITDSAAAGTAMATGYKTTVGTIARNDRHSADLTTIAEAARDHGFGVGIVSSVSIDHATPACFYAHADSRNE
YGAIARQMAQSGFDYFGGGYALSALRDDTLDDELRRLMLQKGYTIAAGKEAIEAALPGSRCWAVGAVDSKGALAYEIDRN
EEDADLAFFTRYGIRLLDHHRGFFLMVEGGKIDWACHANDGATVSREVKAFDRAIGEALAFYRSHPDETLIIVTADHECG
GLALGNNQVRYSGRPSLLDYQQLSLERFSAKVRAWSGSGRVSFPMAMDSLRVYFGLGNIDLDPLLALSPPDSIDLAKAYQ
ACMTSSEELNTYGRSDPFTSSAGALLNRKAGIGWTTGHHTSVPVPVFALGRGQERFGGSYDNTELAKKMMDLCGLSPVAK
E

Sequences:

>Translated_481_residues
MRRPGRAAYLLFLLFMISALSACRSTADGRQHQPDQQQSFPRYIFYFIGDGMGLAQVALGEALAGEQGGLAMLRMPVTGL
MTTYALDRSITDSAAAGTAMATGYKTTVGTIARNDRHSADLTTIAEAARDHGFGVGIVSSVSIDHATPACFYAHADSRNE
YGAIARQMAQSGFDYFGGGYALSALRDDTLDDELRRLMLQKGYTIAAGKEAIEAALPGSRCWAVGAVDSKGALAYEIDRN
EEDADLAFFTRYGIRLLDHHRGFFLMVEGGKIDWACHANDGATVSREVKAFDRAIGEALAFYRSHPDETLIIVTADHECG
GLALGNNQVRYSGRPSLLDYQQLSLERFSAKVRAWSGSGRVSFPMAMDSLRVYFGLGNIDLDPLLALSPPDSIDLAKAYQ
ACMTSSEELNTYGRSDPFTSSAGALLNRKAGIGWTTGHHTSVPVPVFALGRGQERFGGSYDNTELAKKMMDLCGLSPVAK
E
>Mature_481_residues
MRRPGRAAYLLFLLFMISALSACRSTADGRQHQPDQQQSFPRYIFYFIGDGMGLAQVALGEALAGEQGGLAMLRMPVTGL
MTTYALDRSITDSAAAGTAMATGYKTTVGTIARNDRHSADLTTIAEAARDHGFGVGIVSSVSIDHATPACFYAHADSRNE
YGAIARQMAQSGFDYFGGGYALSALRDDTLDDELRRLMLQKGYTIAAGKEAIEAALPGSRCWAVGAVDSKGALAYEIDRN
EEDADLAFFTRYGIRLLDHHRGFFLMVEGGKIDWACHANDGATVSREVKAFDRAIGEALAFYRSHPDETLIIVTADHECG
GLALGNNQVRYSGRPSLLDYQQLSLERFSAKVRAWSGSGRVSFPMAMDSLRVYFGLGNIDLDPLLALSPPDSIDLAKAYQ
ACMTSSEELNTYGRSDPFTSSAGALLNRKAGIGWTTGHHTSVPVPVFALGRGQERFGGSYDNTELAKKMMDLCGLSPVAK
E

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=378, Percent_Identity=26.984126984127, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157266292, Length=332, Percent_Identity=30.1204819277108, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI94721246, Length=341, Percent_Identity=30.791788856305, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI294660770, Length=326, Percent_Identity=28.5276073619632, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI157266296, Length=340, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI48994877, Length=330, Percent_Identity=30, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320689, Length=492, Percent_Identity=26.4227642276423, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24651554, Length=352, Percent_Identity=27.8409090909091, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355981, Length=330, Percent_Identity=29.3939393939394, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355151, Length=324, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355149, Length=332, Percent_Identity=29.2168674698795, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI85725200, Length=328, Percent_Identity=27.4390243902439, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI85815833, Length=328, Percent_Identity=27.4390243902439, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921912, Length=341, Percent_Identity=28.1524926686217, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24657842, Length=338, Percent_Identity=27.5147928994083, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18859923, Length=295, Percent_Identity=25.4237288135593, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51951; Mature: 51951

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPGRAAYLLFLLFMISALSACRSTADGRQHQPDQQQSFPRYIFYFIGDGMGLAQVALG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHHHH
EALAGEQGGLAMLRMPVTGLMTTYALDRSITDSAAAGTAMATGYKTTVGTIARNDRHSAD
HHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHCCCHHHCCCCHHHHHHCCCCCCCCH
LTTIAEAARDHGFGVGIVSSVSIDHATPACFYAHADSRNEYGAIARQMAQSGFDYFGGGY
HHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
ALSALRDDTLDDELRRLMLQKGYTIAAGKEAIEAALPGSRCWAVGAVDSKGALAYEIDRN
EEHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
EEDADLAFFTRYGIRLLDHHRGFFLMVEGGKIDWACHANDGATVSREVKAFDRAIGEALA
CCCCHHHHHHHHHHHEEECCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
FYRSHPDETLIIVTADHECGGLALGNNQVRYSGRPSLLDYQQLSLERFSAKVRAWSGSGR
HHHCCCCCEEEEEEECCCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHEEEECCCCC
VSFPMAMDSLRVYFGLGNIDLDPLLALSPPDSIDLAKAYQACMTSSEELNTYGRSDPFTS
EEECHHHHCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCC
SAGALLNRKAGIGWTTGHHTSVPVPVFALGRGQERFGGSYDNTELAKKMMDLCGLSPVAK
CHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCHHCC
E
C
>Mature Secondary Structure
MRRPGRAAYLLFLLFMISALSACRSTADGRQHQPDQQQSFPRYIFYFIGDGMGLAQVALG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHHHH
EALAGEQGGLAMLRMPVTGLMTTYALDRSITDSAAAGTAMATGYKTTVGTIARNDRHSAD
HHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHCCCHHHCCCCHHHHHHCCCCCCCCH
LTTIAEAARDHGFGVGIVSSVSIDHATPACFYAHADSRNEYGAIARQMAQSGFDYFGGGY
HHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
ALSALRDDTLDDELRRLMLQKGYTIAAGKEAIEAALPGSRCWAVGAVDSKGALAYEIDRN
EEHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
EEDADLAFFTRYGIRLLDHHRGFFLMVEGGKIDWACHANDGATVSREVKAFDRAIGEALA
CCCCHHHHHHHHHHHEEECCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
FYRSHPDETLIIVTADHECGGLALGNNQVRYSGRPSLLDYQQLSLERFSAKVRAWSGSGR
HHHCCCCCEEEEEEECCCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHEEEECCCCC
VSFPMAMDSLRVYFGLGNIDLDPLLALSPPDSIDLAKAYQACMTSSEELNTYGRSDPFTS
EEECHHHHCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCC
SAGALLNRKAGIGWTTGHHTSVPVPVFALGRGQERFGGSYDNTELAKKMMDLCGLSPVAK
CHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]