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The map label for this gene is prtF [H]

Identifier: 194334992

GI number: 194334992

Start: 2375705

End: 2377030

Strand: Reverse

Name: prtF [H]

Synonym: Paes_2203

Alternate gene names: 194334992

Gene position: 2377030-2375705 (Counterclockwise)

Preceding gene: 194334993

Following gene: 194334991

Centisome position: 94.59

GC content: 55.2

Gene sequence:

>1326_bases
ATGACCAAACCATTGATGTTACTGATGCTTTCTGTCCTCTGCGGGCTCGGGAGCGCATCGGCTGAATCGCTTGATCTTCG
GGGTGCATGGGAGCAGGCCCGCTCCTATGACGCCAGGCTTCGTGTTGCAGAAGCGGAGTATGCCATGCAGCAGGAGGAGA
TCGCTAAGGCGCGTGCAGCCTTGCGTCCCAACATTCAGGCGCAGAGCTCCCGCGGAAGAAACAGTACCCGCAGCAGTTCG
CTGGCTGGCACCAGCTACTACAATACCGAGTCGAGCAGTATCACTCTGAAGCAGTCGCTTTTTAACCTGGGAAGTGCTGC
GTCATACAAGCAGGCGAAGGCGATTGCGGCAAAAAGCGGCGAGATACTTCGCAATGAGTCGAATGACCTGATGGTGCGCA
CCGTTGAGGCGTACTTCAATGTGCTGTATGCTGAAGAAGGAGTCGAGGTGAGCCGGGCGAGGGTTGCCGCTGCTGCTGAA
AGGCTGAAACAGGCCCGACGGGCATTGAAAAACGGTCTCGGGACAGTGACTGCAGTTGACGAAGCAGAGGCCCGTTATGA
CAGTGTTGTAGCCGATGAGATAGAAGCCCGTAACAGCGATGAGTTCAACCGGCGCGAGCTGGAGCGCCTGACAGGAATCT
ATGCGCGTGAGTTGCGGCCTCTTTCGGCAGGGTTACTGCCTCTTGACGATCCAGACCCTGTTGATGTCGACGTGTGGCTT
GCCCTTGCTATGGAAAAAAGCCCGGAGATCGGTGCCGCTGAAGAGGGGATGAACATCGCTCGTCGTGAGCTGGAGAAGAA
TCGGGGAGCAGCCTATCCTTCTCTTGACCTTGTTGCAGGAAGGAGTTACTCGGTCAGTGAGACCAGTTATACTATCGACC
GGGCTTATGATACCTATTCGTTGATGCTTCAGTTAAAGGTGCCGATGTATACGGGCGGCTATATCAGCGCCTCGGTGCGC
CAGGCTAGAGTTGGCTGTGTCAAGGCGGAAGAGGAGTTCTCCTGGTATGAGCGCCAGGTCGTTGCCGATATCCGCAGGTA
CTACCATGCCGTGCTGAATGCTGTTCTGCAGGTCAGAGCGTATGAAAAAGCGGTCATTTCCAGAGAAACAGCTTGCAGGA
GTACGAAAAAAGGGGTGCAGGCCGGTCTTTCCACCAGAGTCGATGTGCTTGAATCCCGGGTGAAACTGCTTACGTCCAGG
CAGGATCTTGCTAAAGCCCGCTACCAGTATATTATCAATCTTCTCATGCTCAAGAAAGCGTCCGGTCTCGTTTCCGAGTC
GGATATCGATGAGATCCAGAGCTGGCTGAAGAGCGGGGAGGCATAG

Upstream 100 bases:

>100_bases
GTGCAGACGGTGATCCGTACCGGTGAACGCACGCTGCTGACCTATCTGCTGCATCCGCTGGTCAAACGGATAACCGCATC
TTTGAAGGAGGAGTAGGAAG

Downstream 100 bases:

>100_bases
TCCGTCCGATGCGGTTTTCGTGTGCGGCGCGGTATGCGAAGAAGTGCTTTTTTCGGCGGTTAGGCAGAGAATGGTTACAT
TCAGCAGTAGAGACCGTTGG

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTKPLMLLMLSVLCGLGSASAESLDLRGAWEQARSYDARLRVAEAEYAMQQEEIAKARAALRPNIQAQSSRGRNSTRSSS
LAGTSYYNTESSSITLKQSLFNLGSAASYKQAKAIAAKSGEILRNESNDLMVRTVEAYFNVLYAEEGVEVSRARVAAAAE
RLKQARRALKNGLGTVTAVDEAEARYDSVVADEIEARNSDEFNRRELERLTGIYARELRPLSAGLLPLDDPDPVDVDVWL
ALAMEKSPEIGAAEEGMNIARRELEKNRGAAYPSLDLVAGRSYSVSETSYTIDRAYDTYSLMLQLKVPMYTGGYISASVR
QARVGCVKAEEEFSWYERQVVADIRRYYHAVLNAVLQVRAYEKAVISRETACRSTKKGVQAGLSTRVDVLESRVKLLTSR
QDLAKARYQYIINLLMLKKASGLVSESDIDEIQSWLKSGEA

Sequences:

>Translated_441_residues
MTKPLMLLMLSVLCGLGSASAESLDLRGAWEQARSYDARLRVAEAEYAMQQEEIAKARAALRPNIQAQSSRGRNSTRSSS
LAGTSYYNTESSSITLKQSLFNLGSAASYKQAKAIAAKSGEILRNESNDLMVRTVEAYFNVLYAEEGVEVSRARVAAAAE
RLKQARRALKNGLGTVTAVDEAEARYDSVVADEIEARNSDEFNRRELERLTGIYARELRPLSAGLLPLDDPDPVDVDVWL
ALAMEKSPEIGAAEEGMNIARRELEKNRGAAYPSLDLVAGRSYSVSETSYTIDRAYDTYSLMLQLKVPMYTGGYISASVR
QARVGCVKAEEEFSWYERQVVADIRRYYHAVLNAVLQVRAYEKAVISRETACRSTKKGVQAGLSTRVDVLESRVKLLTSR
QDLAKARYQYIINLLMLKKASGLVSESDIDEIQSWLKSGEA
>Mature_440_residues
TKPLMLLMLSVLCGLGSASAESLDLRGAWEQARSYDARLRVAEAEYAMQQEEIAKARAALRPNIQAQSSRGRNSTRSSSL
AGTSYYNTESSSITLKQSLFNLGSAASYKQAKAIAAKSGEILRNESNDLMVRTVEAYFNVLYAEEGVEVSRARVAAAAER
LKQARRALKNGLGTVTAVDEAEARYDSVVADEIEARNSDEFNRRELERLTGIYARELRPLSAGLLPLDDPDPVDVDVWLA
LAMEKSPEIGAAEEGMNIARRELEKNRGAAYPSLDLVAGRSYSVSETSYTIDRAYDTYSLMLQLKVPMYTGGYISASVRQ
ARVGCVKAEEEFSWYERQVVADIRRYYHAVLNAVLQVRAYEKAVISRETACRSTKKGVQAGLSTRVDVLESRVKLLTSRQ
DLAKARYQYIINLLMLKKASGLVSESDIDEIQSWLKSGEA

Specific function: Involved in the secretion of proteases A, B, C and G [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=441, Percent_Identity=28.7981859410431, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48841; Mature: 48710

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLMLLMLSVLCGLGSASAESLDLRGAWEQARSYDARLRVAEAEYAMQQEEIAKARAA
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHEEEHHHHHHHHHHHHHHHHHH
LRPNIQAQSSRGRNSTRSSSLAGTSYYNTESSSITLKQSLFNLGSAASYKQAKAIAAKSG
HCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEHHHHHHHCCCCCHHHHHHHHHHCCC
EILRNESNDLMVRTVEAYFNVLYAEEGVEVSRARVAAAAERLKQARRALKNGLGTVTAVD
HHHHCCCCCHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECH
EAEARYDSVVADEIEARNSDEFNRRELERLTGIYARELRPLSAGLLPLDDPDPVDVDVWL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHH
ALAMEKSPEIGAAEEGMNIARRELEKNRGAAYPSLDLVAGRSYSVSETSYTIDRAYDTYS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEHHHHHCCEE
LMLQLKVPMYTGGYISASVRQARVGCVKAEEEFSWYERQVVADIRRYYHAVLNAVLQVRA
EEEEEECCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKAVISRETACRSTKKGVQAGLSTRVDVLESRVKLLTSRQDLAKARYQYIINLLMLKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVSESDIDEIQSWLKSGEA
CCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TKPLMLLMLSVLCGLGSASAESLDLRGAWEQARSYDARLRVAEAEYAMQQEEIAKARAA
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHEEEHHHHHHHHHHHHHHHHHH
LRPNIQAQSSRGRNSTRSSSLAGTSYYNTESSSITLKQSLFNLGSAASYKQAKAIAAKSG
HCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEHHHHHHHCCCCCHHHHHHHHHHCCC
EILRNESNDLMVRTVEAYFNVLYAEEGVEVSRARVAAAAERLKQARRALKNGLGTVTAVD
HHHHCCCCCHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECH
EAEARYDSVVADEIEARNSDEFNRRELERLTGIYARELRPLSAGLLPLDDPDPVDVDVWL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHH
ALAMEKSPEIGAAEEGMNIARRELEKNRGAAYPSLDLVAGRSYSVSETSYTIDRAYDTYS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEHHHHHCCEE
LMLQLKVPMYTGGYISASVRQARVGCVKAEEEFSWYERQVVADIRRYYHAVLNAVLQVRA
EEEEEECCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKAVISRETACRSTKKGVQAGLSTRVDVLESRVKLLTSRQDLAKARYQYIINLLMLKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVSESDIDEIQSWLKSGEA
CCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2184029 [H]