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The map label for this gene is tuf [H]

Identifier: 194334863

GI number: 194334863

Start: 2249064

End: 2250245

Strand: Reverse

Name: tuf [H]

Synonym: Paes_2066

Alternate gene names: 194334863

Gene position: 2250245-2249064 (Counterclockwise)

Preceding gene: 194334864

Following gene: 194334862

Centisome position: 89.55

GC content: 50.08

Gene sequence:

>1182_bases
ATGGCAAAAGAGGCCTACAAGAGGGAGAAACCTCATGTAAATATTGGTACCATCGGTCACGTTGACCATGGTAAAACAAC
ACTGACAGCTGCAATTACCACAGTTCTTGCAAAGAAAGGTATGGCTCAGTTGCGCGAATTCGGTGACATCGATAAGGCGC
CTGAAGAAAGAGAGCGCGGTATTACCATTTCTACCGCACACGTTGAATACGAGACCGACAAACGTCACTATGCGCACATT
GACTGTCCAGGTCACGCTGACTACATCAAGAATATGATTACCGGTGCTGCTCAGATGGACGGTGCTATTCTCGTCGTTGC
CGGTACCGATGGTCCTATGCCTCAGACTCGCGAGCACATTCTGCTTGCCCGTCAGGTGAACGTTCCTGCTCTTGTTGTTT
TCCTCAACAAAGTAGATATCGCCGATCCCGAGCTTATCGAGCTTGTCGAGCTTGAACTTCGCGAGCTGCTCTCCGAGTAT
GAGTTCCCTGGTGACGATATCCCGATCATCAAAGGCTCGGCACTGAAAGCCCTTGATGGCGACCCTGAATCAGAGGCTGC
TATCATGGAACTTATGGACGCAGTTGACAGCTATATTCCTGAGCCTGTCCGCGACGTTGACAAGCCGTTCCTGATGCCTA
TCGAGGACGTTTTCTCTATCTCCGGTCGTGGCACTGTTGGTACAGGCCGTATCGAGAGTGGTGTTATTAAAATCGGCGAA
GAGGTCGAAATCGTCGGGATCAAGCCGACCCGTAAATCAGTTGTTACCGGTATCGAGATGTTCCAGAAAACTCTTGACCA
GGGACAGGCTGGTGATAACGCAGGTATTCTTTTCCGCGGTGTCGATAAGGAAGAGCTTGAGCGTGGAATGGTTATCGCTA
AACCGGGCACCATCACTCCTCATACCAAATTCAAGGCAGAGGTGTATATCCTTAAAAAAGAAGAGGGTGGCCGCCATACC
CCATTCTTCAACAACTATCGTCCCCAGTTCTATTTCAGAACCACTGACGTTACCGGTGCAGTTACTCTGCCCGAAGGTGT
TGAAATGGTTATGCCTGGTGACAACCTTTCTGTCGAAGTCGAGCTTATCGTTCCTATCGCTATGGACGAGAACCTTCGCT
TCGCTATTCGTGAAGGTGGCCGTACCGTCGGTGCAGGTACTGTTACCCAGATTATCGAGTGA

Upstream 100 bases:

>100_bases
GAAGAACAGCGCTAAGAATGCTGACTGAGTGATTTAGGATCAAGACGTATTACTGTATTACATAAAACAACAACTGTTAA
CCGATAGAGGGAGAGGAAAC

Downstream 100 bases:

>100_bases
TAGAAAAATGATGTTCGTGATGGGGCGGGGATATCGTTCCGCCCCATTTTTATTGTAAAACATTGAAAGAGGTTTAGATA
AGTGGCTGTTCAGCAAAAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAKEAYKREKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAQLREFGDIDKAPEERERGITISTAHVEYETDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELIELVELELRELLSEY
EFPGDDIPIIKGSALKALDGDPESEAAIMELMDAVDSYIPEPVRDVDKPFLMPIEDVFSISGRGTVGTGRIESGVIKIGE
EVEIVGIKPTRKSVVTGIEMFQKTLDQGQAGDNAGILFRGVDKEELERGMVIAKPGTITPHTKFKAEVYILKKEEGGRHT
PFFNNYRPQFYFRTTDVTGAVTLPEGVEMVMPGDNLSVEVELIVPIAMDENLRFAIREGGRTVGAGTVTQIIE

Sequences:

>Translated_393_residues
MAKEAYKREKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAQLREFGDIDKAPEERERGITISTAHVEYETDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELIELVELELRELLSEY
EFPGDDIPIIKGSALKALDGDPESEAAIMELMDAVDSYIPEPVRDVDKPFLMPIEDVFSISGRGTVGTGRIESGVIKIGE
EVEIVGIKPTRKSVVTGIEMFQKTLDQGQAGDNAGILFRGVDKEELERGMVIAKPGTITPHTKFKAEVYILKKEEGGRHT
PFFNNYRPQFYFRTTDVTGAVTLPEGVEMVMPGDNLSVEVELIVPIAMDENLRFAIREGGRTVGAGTVTQIIE
>Mature_392_residues
AKEAYKREKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAQLREFGDIDKAPEERERGITISTAHVEYETDKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELIELVELELRELLSEYE
FPGDDIPIIKGSALKALDGDPESEAAIMELMDAVDSYIPEPVRDVDKPFLMPIEDVFSISGRGTVGTGRIESGVIKIGEE
VEIVGIKPTRKSVVTGIEMFQKTLDQGQAGDNAGILFRGVDKEELERGMVIAKPGTITPHTKFKAEVYILKKEEGGRHTP
FFNNYRPQFYFRTTDVTGAVTLPEGVEMVMPGDNLSVEVELIVPIAMDENLRFAIREGGRTVGAGTVTQIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.3451776649746, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=31.8906605922551, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=31.8906605922551, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.5964125560538, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=29.1479820627803, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=34.1614906832298, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.4733727810651, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=30.4733727810651, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.4733727810651, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=29.7142857142857, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=283, Percent_Identity=28.6219081272085, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI157426893, Length=239, Percent_Identity=28.8702928870293, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=34.3283582089552, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=72.0101781170484, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=72.0101781170484, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=34.0248962655602, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=280, Percent_Identity=27.8571428571429, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=332, Percent_Identity=27.1084337349398, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=384, Percent_Identity=44.7916666666667, Blast_Score=337, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=31.3199105145414, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=31.3199105145414, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=424, Percent_Identity=30.6603773584906, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=447, Percent_Identity=28.6353467561521, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=447, Percent_Identity=28.6353467561521, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=343, Percent_Identity=29.7376093294461, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=31.2080536912752, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=43.5374149659864, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=28.7234042553192, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=29.3103448275862, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=195, Percent_Identity=34.3589743589744, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556745, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=134, Percent_Identity=35.0746268656716, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=67.5062972292191, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.0225733634311, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.0225733634311, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=458, Percent_Identity=24.235807860262, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=28.9017341040462, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=285, Percent_Identity=29.8245614035088, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=169, Percent_Identity=32.5443786982249, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=56.6326530612245, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=56.6326530612245, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.4455958549223, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=31.0421286031042, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=31.0421286031042, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=31.0421286031042, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=31.0421286031042, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.8724832214765, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.8724832214765, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=30.9210526315789, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550900, Length=347, Percent_Identity=29.3948126801153, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=28.448275862069, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=29.0322580645161, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=29.0322580645161, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=175, Percent_Identity=36.5714285714286, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI78706572, Length=244, Percent_Identity=27.8688524590164, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43100; Mature: 42969

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEAYKREKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAQLREFGDIDKAPEERERG
CCCHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
ITISTAHVEYETDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELIELVELELRELLSEYEFPGDDIPIIKGSALKALDG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEECCCC
DPESEAAIMELMDAVDSYIPEPVRDVDKPFLMPIEDVFSISGRGTVGTGRIESGVIKIGE
CCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECC
EVEIVGIKPTRKSVVTGIEMFQKTLDQGQAGDNAGILFRGVDKEELERGMVIAKPGTITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNNYRPQFYFRTTDVTGAVTLPEGVEMVMPGDNLSVEV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEEE
ELIVPIAMDENLRFAIREGGRTVGAGTVTQIIE
EEEEEEEECCCCEEEEECCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKEAYKREKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAQLREFGDIDKAPEERERG
CCHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
ITISTAHVEYETDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELIELVELELRELLSEYEFPGDDIPIIKGSALKALDG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEECCCC
DPESEAAIMELMDAVDSYIPEPVRDVDKPFLMPIEDVFSISGRGTVGTGRIESGVIKIGE
CCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECC
EVEIVGIKPTRKSVVTGIEMFQKTLDQGQAGDNAGILFRGVDKEELERGMVIAKPGTITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNNYRPQFYFRTTDVTGAVTLPEGVEMVMPGDNLSVEV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEEE
ELIVPIAMDENLRFAIREGGRTVGAGTVTQIIE
EEEEEEEECCCCEEEEECCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8085791 [H]