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The map label for this gene is purA [H]

Identifier: 194334828

GI number: 194334828

Start: 2227807

End: 2229114

Strand: Reverse

Name: purA [H]

Synonym: Paes_2031

Alternate gene names: 194334828

Gene position: 2229114-2227807 (Counterclockwise)

Preceding gene: 194334829

Following gene: 194334827

Centisome position: 88.71

GC content: 51.91

Gene sequence:

>1308_bases
GTGAAAGAAAACACAGCCAGGCAGCAGACACCTGCTTCCGCAACCGTTATTGTAGGAACCCAGTTCGGAGATGAAGGAAA
AGGCAAGCTGGTGGACTTTCTCTCTGCCAGATTTGATATTGTCGTACGCTATCAGGGAGGGGCCAACGCAGGCCATACCA
TCTGTTTCGATGACAAGACCGTGGTGCTTCACCTCATCCCGTCAGGAATTTTTCATGAAGGGTGTACCTGCGTCATTGGC
AATGGTGTTGTCATTGATCCCAATGCCCTGCTTGAAGAGATTAGGCAGGTCGAAGAACTCGGCTACGATGTGCAGGGCAG
GCTGTTTATCAGTCATAATGCGCATCTTATCATGCCGTACCATAAACTGCTTGACTCACTGCACGAAAATGCGCAGAACG
CCAGGAAAATCGGCACCACCGGCAGGGGAATAGGTCCGAGCTATGAAGATAAATTCGCGCGAAAAGGTATTCGCGTTGTC
GATCTTCTGCATCCGGAGCTGCTCGAAGAGAAGCTTCGCGACAATCTTGAAGCAAAAAACAAGCTTATTCGTAACGTCTA
TGACAACGAAGGCCTCGATGTCGATAAGATGGTCCGCGAGTATTCCGAGTTTGACGCCATCATCAATCCCTATGTCAAAA
ATACCCAGCTCTATCTCAATAAGCAGCTCAGGGCGGGAAAAAGCATTTTGCTCGAAGGGGCACAGGGCTGCCTGCTTGAT
GTCGACCACGGCACCTACCCCTATGTCACCTCATCAAACCCCACGTCCGGCGGCGCATCAACCGGCTCGGGTATTGCCCC
AAACTATATCAAAAACGTCATCGGTGTCAGCAAGGCCTATATGACCAGGGTCGGCAACGGTGCTTTCCCGACCGAACTGC
TCGATGAAACCGGCGAAAAGCTTGGCAAGATCGGTCACGAATTCGGGGCGACCACAGGCCGCAGGCGCCGCTGCGGGTGG
CTTGACCTGGTTGCACTGCGCTATTCGCTTATCGTGAACGGGGTAACTGAAATCGCCCTGACCAAGCTCGATGTGCTCGA
TGCGTTTGACGAAATCAAAGTCTGTACCTCATACATGCTTGATGGTCGTGAAATTGTCGATTTTCCGACCGATGACCAGA
CCCTCTCAAAGGTTAAGCCTGTCTACAAAACATTCAAGGGATGGAATGTTTCCAATGCCGGTGCATCGACATTCGAAGAG
ATGCATCCCTCGACGAGAGCCTATATCAGCTTCCTTGAAGAGGAACTTGAGGTTCCCGTGACCTTCATTTCCGTCGGCCC
GGGCCGCGACGAAACCGTTTTCCGCTAG

Upstream 100 bases:

>100_bases
GAAAACCAAATTTATTGTAAATTACTCGGCTCTGAGCGGAGAGGGGAAAGAGAGCCTCCTTGAGCAATTAGAAAACTATA
CAGGTTAAAACAGGATTGTC

Downstream 100 bases:

>100_bases
CCTGCAAGGGGGCCGCATGGCACTTGTCGATATTACCATTATTCCTCTTGACGGACGATCCGGGGGGTTAGGCGCCCTTG
TGGCGGAGCTTGAAGCGCTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKENTARQQTPASATVIVGTQFGDEGKGKLVDFLSARFDIVVRYQGGANAGHTICFDDKTVVLHLIPSGIFHEGCTCVIG
NGVVIDPNALLEEIRQVEELGYDVQGRLFISHNAHLIMPYHKLLDSLHENAQNARKIGTTGRGIGPSYEDKFARKGIRVV
DLLHPELLEEKLRDNLEAKNKLIRNVYDNEGLDVDKMVREYSEFDAIINPYVKNTQLYLNKQLRAGKSILLEGAQGCLLD
VDHGTYPYVTSSNPTSGGASTGSGIAPNYIKNVIGVSKAYMTRVGNGAFPTELLDETGEKLGKIGHEFGATTGRRRRCGW
LDLVALRYSLIVNGVTEIALTKLDVLDAFDEIKVCTSYMLDGREIVDFPTDDQTLSKVKPVYKTFKGWNVSNAGASTFEE
MHPSTRAYISFLEEELEVPVTFISVGPGRDETVFR

Sequences:

>Translated_435_residues
MKENTARQQTPASATVIVGTQFGDEGKGKLVDFLSARFDIVVRYQGGANAGHTICFDDKTVVLHLIPSGIFHEGCTCVIG
NGVVIDPNALLEEIRQVEELGYDVQGRLFISHNAHLIMPYHKLLDSLHENAQNARKIGTTGRGIGPSYEDKFARKGIRVV
DLLHPELLEEKLRDNLEAKNKLIRNVYDNEGLDVDKMVREYSEFDAIINPYVKNTQLYLNKQLRAGKSILLEGAQGCLLD
VDHGTYPYVTSSNPTSGGASTGSGIAPNYIKNVIGVSKAYMTRVGNGAFPTELLDETGEKLGKIGHEFGATTGRRRRCGW
LDLVALRYSLIVNGVTEIALTKLDVLDAFDEIKVCTSYMLDGREIVDFPTDDQTLSKVKPVYKTFKGWNVSNAGASTFEE
MHPSTRAYISFLEEELEVPVTFISVGPGRDETVFR
>Mature_435_residues
MKENTARQQTPASATVIVGTQFGDEGKGKLVDFLSARFDIVVRYQGGANAGHTICFDDKTVVLHLIPSGIFHEGCTCVIG
NGVVIDPNALLEEIRQVEELGYDVQGRLFISHNAHLIMPYHKLLDSLHENAQNARKIGTTGRGIGPSYEDKFARKGIRVV
DLLHPELLEEKLRDNLEAKNKLIRNVYDNEGLDVDKMVREYSEFDAIINPYVKNTQLYLNKQLRAGKSILLEGAQGCLLD
VDHGTYPYVTSSNPTSGGASTGSGIAPNYIKNVIGVSKAYMTRVGNGAFPTELLDETGEKLGKIGHEFGATTGRRRRCGW
LDLVALRYSLIVNGVTEIALTKLDVLDAFDEIKVCTSYMLDGREIVDFPTDDQTLSKVKPVYKTFKGWNVSNAGASTFEE
MHPSTRAYISFLEEELEVPVTFISVGPGRDETVFR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=44.988344988345, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=44.4186046511628, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=44.5569620253165, Blast_Score=321, Evalue=9e-88,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=49.7619047619048, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.1860465116279, Blast_Score=357, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.1860465116279, Blast_Score=357, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=42.92343387471, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=44.1805225653207, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48040; Mature: 48040

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENTARQQTPASATVIVGTQFGDEGKGKLVDFLSARFDIVVRYQGGANAGHTICFDDKT
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCEEEECCCE
VVLHLIPSGIFHEGCTCVIGNGVVIDPNALLEEIRQVEELGYDVQGRLFISHNAHLIMPY
EEEEECCCHHHHCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHH
HKLLDSLHENAQNARKIGTTGRGIGPSYEDKFARKGIRVVDLLHPELLEEKLRDNLEAKN
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEHHCHHHHHHHHHHHHHHHH
KLIRNVYDNEGLDVDKMVREYSEFDAIINPYVKNTQLYLNKQLRAGKSILLEGAQGCLLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHCCCHHEEHHHHHCCCHHEEECCCCCEEE
VDHGTYPYVTSSNPTSGGASTGSGIAPNYIKNVIGVSKAYMTRVGNGAFPTELLDETGEK
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LGKIGHEFGATTGRRRRCGWLDLVALRYSLIVNGVTEIALTKLDVLDAFDEIKVCTSYML
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGREIVDFPTDDQTLSKVKPVYKTFKGWNVSNAGASTFEEMHPSTRAYISFLEEELEVPV
CCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCE
TFISVGPGRDETVFR
EEEEECCCCCCCCCC
>Mature Secondary Structure
MKENTARQQTPASATVIVGTQFGDEGKGKLVDFLSARFDIVVRYQGGANAGHTICFDDKT
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCEEEECCCE
VVLHLIPSGIFHEGCTCVIGNGVVIDPNALLEEIRQVEELGYDVQGRLFISHNAHLIMPY
EEEEECCCHHHHCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHH
HKLLDSLHENAQNARKIGTTGRGIGPSYEDKFARKGIRVVDLLHPELLEEKLRDNLEAKN
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEHHCHHHHHHHHHHHHHHHH
KLIRNVYDNEGLDVDKMVREYSEFDAIINPYVKNTQLYLNKQLRAGKSILLEGAQGCLLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHCCCHHEEHHHHHCCCHHEEECCCCCEEE
VDHGTYPYVTSSNPTSGGASTGSGIAPNYIKNVIGVSKAYMTRVGNGAFPTELLDETGEK
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LGKIGHEFGATTGRRRRCGWLDLVALRYSLIVNGVTEIALTKLDVLDAFDEIKVCTSYML
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGREIVDFPTDDQTLSKVKPVYKTFKGWNVSNAGASTFEEMHPSTRAYISFLEEELEVPV
CCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCE
TFISVGPGRDETVFR
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA