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The map label for this gene is dfrA [H]

Identifier: 194334751

GI number: 194334751

Start: 2133617

End: 2134642

Strand: Reverse

Name: dfrA [H]

Synonym: Paes_1952

Alternate gene names: 194334751

Gene position: 2134642-2133617 (Counterclockwise)

Preceding gene: 194334754

Following gene: 194334750

Centisome position: 84.95

GC content: 55.17

Gene sequence:

>1026_bases
ATGCAGGATAAAGATACCTTGGGACAAAGCGGGCGGGTTATACTGGTTACCGGAGCGACCGGTTATATAGGATCGGAGTT
GGTTGAAAGGCTCAGTTCCCTCTGCGATGCCGGGTTGCATCTCAGGGTGCTTGCTCGCAGAGGATCCGATGTTTCGGTTC
TTGACGGCAACAGCGTTGAGTTTGTGTACGGTGATCTTCTTGATTCCCTTTCCCTCTATGATGCCTGCAGCGGGGTCGAT
ACGGTTTTTCATTGCGCAGGTTTGATCGCCTACAGCCGAAACTACCGGCAGCGGCTTTATGCAACCAATGTTACCGGAAC
CGGCAATCTGGTCAATGCCTGTCTGGCTGAGGGTGTCACACGGCTGGTCCACACCAGTTCAGTCGCGGCGGCAGGGGTTG
GCGATGATGGTGAGCCGGCCGATGAAACAACGCCGTTCAGGGAGTGGCAGCACAGGATTGCATATATGGAGTCGAAACAT
CTTGCCGAGATGGAAGGGCGGAGAGGTATTGCAGAAGGGCTGGATGTGGTGTTTGTCAATCCCGGCGTCGTGATCGGCAC
TCCTTCCAACCCGGCAGGACGTTTGAACTCCTCTTCCAGGGCGATTCGTGATATCTATCGTGGTACAATTCCTGTGTATC
CTTCAGGAGGCATCAGCCTGGTCGATATCGGCGATGTTGCCGATGCCCACCTTGCCGCATGGCGGCATGGCCGGAGCGGA
CAGCGCTATCTGGTGACTGCAGGGAATTATTCGTTCCGCCAGTTGTTTGCCATGATCGCCGCAATGCCGGGAAGCGCTGC
CGGAAAGGCTTATCAGGCGGCATCTGTCGTGGAGTCGGTCGTTGGTGTGGCGGGTGAGTTGTTTGCTCTCCTGACAGGCT
CCAGGCCCTATCTCAGCGTCGAAAGTATGAGGCTTGCACGAACGTTACTGTATTACAGCAACCGTCTGTCTGTCGAAGAA
CTTGGCATGCAATATCGCCCGGTTGAAGAGACACTTCGCTCGATTGTTTCTCAAGCGGCAGTATAA

Upstream 100 bases:

>100_bases
TGTTCATAAGGGCAAACTTTTTTTTGACGTCTGATGTTTGATTTGAATGCAATGTAGTTTTTCTTCTTATTTTTAAGAAC
AGCAAGTGCAAGAGCGGCTA

Downstream 100 bases:

>100_bases
GCGGGGGTCGAAGTTTTTTTCCTGTCAATCATCATTGAGAGAAGAGTAATTCTATGAATACACAGAAAACAGCAGTGAAT
AATAAAGAGATGGATCCGGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQDKDTLGQSGRVILVTGATGYIGSELVERLSSLCDAGLHLRVLARRGSDVSVLDGNSVEFVYGDLLDSLSLYDACSGVD
TVFHCAGLIAYSRNYRQRLYATNVTGTGNLVNACLAEGVTRLVHTSSVAAAGVGDDGEPADETTPFREWQHRIAYMESKH
LAEMEGRRGIAEGLDVVFVNPGVVIGTPSNPAGRLNSSSRAIRDIYRGTIPVYPSGGISLVDIGDVADAHLAAWRHGRSG
QRYLVTAGNYSFRQLFAMIAAMPGSAAGKAYQAASVVESVVGVAGELFALLTGSRPYLSVESMRLARTLLYYSNRLSVEE
LGMQYRPVEETLRSIVSQAAV

Sequences:

>Translated_341_residues
MQDKDTLGQSGRVILVTGATGYIGSELVERLSSLCDAGLHLRVLARRGSDVSVLDGNSVEFVYGDLLDSLSLYDACSGVD
TVFHCAGLIAYSRNYRQRLYATNVTGTGNLVNACLAEGVTRLVHTSSVAAAGVGDDGEPADETTPFREWQHRIAYMESKH
LAEMEGRRGIAEGLDVVFVNPGVVIGTPSNPAGRLNSSSRAIRDIYRGTIPVYPSGGISLVDIGDVADAHLAAWRHGRSG
QRYLVTAGNYSFRQLFAMIAAMPGSAAGKAYQAASVVESVVGVAGELFALLTGSRPYLSVESMRLARTLLYYSNRLSVEE
LGMQYRPVEETLRSIVSQAAV
>Mature_341_residues
MQDKDTLGQSGRVILVTGATGYIGSELVERLSSLCDAGLHLRVLARRGSDVSVLDGNSVEFVYGDLLDSLSLYDACSGVD
TVFHCAGLIAYSRNYRQRLYATNVTGTGNLVNACLAEGVTRLVHTSSVAAAGVGDDGEPADETTPFREWQHRIAYMESKH
LAEMEGRRGIAEGLDVVFVNPGVVIGTPSNPAGRLNSSSRAIRDIYRGTIPVYPSGGISLVDIGDVADAHLAAWRHGRSG
QRYLVTAGNYSFRQLFAMIAAMPGSAAGKAYQAASVVESVVGVAGELFALLTGSRPYLSVESMRLARTLLYYSNRLSVEE
LGMQYRPVEETLRSIVSQAAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=167, Percent_Identity=31.7365269461078, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI8393516, Length=167, Percent_Identity=31.7365269461078, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI310132178, Length=131, Percent_Identity=33.587786259542, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310113012, Length=131, Percent_Identity=33.587786259542, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI239745448, Length=131, Percent_Identity=33.587786259542, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=254, Percent_Identity=31.8897637795276, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36490; Mature: 36490

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKDTLGQSGRVILVTGATGYIGSELVERLSSLCDAGLHLRVLARRGSDVSVLDGNSVE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEE
FVYGDLLDSLSLYDACSGVDTVFHCAGLIAYSRNYRQRLYATNVTGTGNLVNACLAEGVT
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RLVHTSSVAAAGVGDDGEPADETTPFREWQHRIAYMESKHLAEMEGRRGIAEGLDVVFVN
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEC
PGVVIGTPSNPAGRLNSSSRAIRDIYRGTIPVYPSGGISLVDIGDVADAHLAAWRHGRSG
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCC
QRYLVTAGNYSFRQLFAMIAAMPGSAAGKAYQAASVVESVVGVAGELFALLTGSRPYLSV
CEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEH
ESMRLARTLLYYSNRLSVEELGMQYRPVEETLRSIVSQAAV
HHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDKDTLGQSGRVILVTGATGYIGSELVERLSSLCDAGLHLRVLARRGSDVSVLDGNSVE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEE
FVYGDLLDSLSLYDACSGVDTVFHCAGLIAYSRNYRQRLYATNVTGTGNLVNACLAEGVT
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RLVHTSSVAAAGVGDDGEPADETTPFREWQHRIAYMESKHLAEMEGRRGIAEGLDVVFVN
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEC
PGVVIGTPSNPAGRLNSSSRAIRDIYRGTIPVYPSGGISLVDIGDVADAHLAAWRHGRSG
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCC
QRYLVTAGNYSFRQLFAMIAAMPGSAAGKAYQAASVVESVVGVAGELFALLTGSRPYLSV
CEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEH
ESMRLARTLLYYSNRLSVEELGMQYRPVEETLRSIVSQAAV
HHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]