Click here to switch to the map view.

The map label for this gene is rfbK [H]

Identifier: 194334538

GI number: 194334538

Start: 1892273

End: 1893682

Strand: Reverse

Name: rfbK [H]

Synonym: Paes_1733

Alternate gene names: 194334538

Gene position: 1893682-1892273 (Counterclockwise)

Preceding gene: 194334542

Following gene: 194334537

Centisome position: 75.36

GC content: 59.43

Gene sequence:

>1410_bases
ATGAATTTTCTTTTGGGGTCGGGGGTTGGGTTTGGGACGAGTGGGGCTCGGGGGTTGGTGGTGGATATGACTGACCGGGT
GTGTTATGGGTTTGCGGCGGCTTTTCTTGGAAGTGTTGCAGGGGATGCTGCGGGTGTGGTTATCGGGCATGATTTGCGGC
CGAGCAGTCCGGGGATTGCCGGGGCGTGCGCTCGGGCGGTGATGGATTCGGGGCGTGAGCTGGTTTATGCGGGAGCGTTG
CCTACTCCGGCTCTGGCTTTTTATGCACAGCGGCTTGGATTGCCTGCGATTGTGGTGACGGGGAGTCATATTCCGTTTGA
TCGGAACGGGATTAAGTTTTACCGTGCGGATGGGGAGATTTCGAAGGCGGATGAGCTGGCGATGATGGAGGCGGAGGTGG
TTGTGCCGGAGGTGGTTGTTCCGGCGGTTTTGCCGGCTCTTGATGGCCGGGCGCGAGAGGCGTATGTGCGACGATATCTT
GATTTTTTCGGTGCGGGTTCGCTTGAGGGGATGCGTGTCGGGGTGTATGAGCATTCGAGCGTTGCTCGGGATGTGTTGCG
TGAGATTCTTGAGGGGCTTGGCGCTGACGTGGTTTCGCTGGGGCGGACGGAGGTGTTTGTGCCGATCGATACGGAGGCGG
TGCGTCCGGAGGATGTGCTGCAGGCCAGGGCGTGGGCTGATGAGGTTGGTTTTGATGCGATTGTGTCGACTGACGGGGAT
GGTGACCGGCCGTTGATCGGGGATGAGAATGGTTTGTGGATGCGTGGAGATGTTGTTGGTATTTTGTGTGCGCAGTTTTT
GCGTGCCAATGTGGTGGTGACGCCGGTGAGCTGCAATACGGCTGTGGAGGCGTGTGGCTGGTTTGGCAAGGTTGTTCGGA
CGCGTATCGGGTCGCCGTATGTGGTTGCGGGGATGGATGCGTCTGCCGCTGATGGCGTTGTGGTTTGCTATGAGGCTAAT
GGTGGGTTTCTTTTGGAGAGCCGGGTTGTGCATAACGGGCGGACGCTGGAGGCGCTGCCGACGCGCGATTCGGTGCTGCC
GATTCTTGCGTTGCTTGGTATGGCGCGGGAGCGTGATGAGAAGGTTTCCGGGTTGAGCGCGTCGTTGCCGAAGCGGTTTA
CGGCGAGCGATCGGCTGAAGGATGTGCCGACTGAGCGGAGCAATTCGTTGATATCGAGTCTGCGGGATTCGCCGGATGAG
GCGGCCCGGTTGCTTGGGCCTGATGCTGGTGGGGTTGCGTGGATGGATATGACGGATGGATTGCGGATGACGTTTGAGAA
CGGGGATATTATTCATCTGCGACCGTCGGGCAATGCACCGGAGCTGCGGTGTTATGCTGAGGCGGATAGTGTTGAGCGGG
CTGAGATGTTGTGTGCGGGTGCGCTTGGCGCGGTGACGCGTGACGCGTGA

Upstream 100 bases:

>100_bases
TCTTCTCTGTGTTCCTCTGTGTTATCTGTGTTCGTCTGTGTGATCTGTGGGTAAAAAGAGCTATCATTTTTAATAGTAAC
AATTTACTCCTTTATATGTC

Downstream 100 bases:

>100_bases
CGCGTGACGCGTGACAAGGGGCAAGAGACGAGAGGCAAGAGACGAGAGGCAAGAGACGAGAGGCAAGAGACGAGAGGCAA
GAGACGAGAGGCAAGAGACG

Product: phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain-containing protein

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNFLLGSGVGFGTSGARGLVVDMTDRVCYGFAAAFLGSVAGDAAGVVIGHDLRPSSPGIAGACARAVMDSGRELVYAGAL
PTPALAFYAQRLGLPAIVVTGSHIPFDRNGIKFYRADGEISKADELAMMEAEVVVPEVVVPAVLPALDGRAREAYVRRYL
DFFGAGSLEGMRVGVYEHSSVARDVLREILEGLGADVVSLGRTEVFVPIDTEAVRPEDVLQARAWADEVGFDAIVSTDGD
GDRPLIGDENGLWMRGDVVGILCAQFLRANVVVTPVSCNTAVEACGWFGKVVRTRIGSPYVVAGMDASAADGVVVCYEAN
GGFLLESRVVHNGRTLEALPTRDSVLPILALLGMARERDEKVSGLSASLPKRFTASDRLKDVPTERSNSLISSLRDSPDE
AARLLGPDAGGVAWMDMTDGLRMTFENGDIIHLRPSGNAPELRCYAEADSVERAEMLCAGALGAVTRDA

Sequences:

>Translated_469_residues
MNFLLGSGVGFGTSGARGLVVDMTDRVCYGFAAAFLGSVAGDAAGVVIGHDLRPSSPGIAGACARAVMDSGRELVYAGAL
PTPALAFYAQRLGLPAIVVTGSHIPFDRNGIKFYRADGEISKADELAMMEAEVVVPEVVVPAVLPALDGRAREAYVRRYL
DFFGAGSLEGMRVGVYEHSSVARDVLREILEGLGADVVSLGRTEVFVPIDTEAVRPEDVLQARAWADEVGFDAIVSTDGD
GDRPLIGDENGLWMRGDVVGILCAQFLRANVVVTPVSCNTAVEACGWFGKVVRTRIGSPYVVAGMDASAADGVVVCYEAN
GGFLLESRVVHNGRTLEALPTRDSVLPILALLGMARERDEKVSGLSASLPKRFTASDRLKDVPTERSNSLISSLRDSPDE
AARLLGPDAGGVAWMDMTDGLRMTFENGDIIHLRPSGNAPELRCYAEADSVERAEMLCAGALGAVTRDA
>Mature_469_residues
MNFLLGSGVGFGTSGARGLVVDMTDRVCYGFAAAFLGSVAGDAAGVVIGHDLRPSSPGIAGACARAVMDSGRELVYAGAL
PTPALAFYAQRLGLPAIVVTGSHIPFDRNGIKFYRADGEISKADELAMMEAEVVVPEVVVPAVLPALDGRAREAYVRRYL
DFFGAGSLEGMRVGVYEHSSVARDVLREILEGLGADVVSLGRTEVFVPIDTEAVRPEDVLQARAWADEVGFDAIVSTDGD
GDRPLIGDENGLWMRGDVVGILCAQFLRANVVVTPVSCNTAVEACGWFGKVVRTRIGSPYVVAGMDASAADGVVVCYEAN
GGFLLESRVVHNGRTLEALPTRDSVLPILALLGMARERDEKVSGLSASLPKRFTASDRLKDVPTERSNSLISSLRDSPDE
AARLLGPDAGGVAWMDMTDGLRMTFENGDIIHLRPSGNAPELRCYAEADSVERAEMLCAGALGAVTRDA

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1789566, Length=319, Percent_Identity=31.974921630094, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 49553; Mature: 49553

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLLGSGVGFGTSGARGLVVDMTDRVCYGFAAAFLGSVAGDAAGVVIGHDLRPSSPGIA
CCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
GACARAVMDSGRELVYAGALPTPALAFYAQRLGLPAIVVTGSHIPFDRNGIKFYRADGEI
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCC
SKADELAMMEAEVVVPEVVVPAVLPALDGRAREAYVRRYLDFFGAGSLEGMRVGVYEHSS
CCHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
VARDVLREILEGLGADVVSLGRTEVFVPIDTEAVRPEDVLQARAWADEVGFDAIVSTDGD
HHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
GDRPLIGDENGLWMRGDVVGILCAQFLRANVVVTPVSCNTAVEACGWFGKVVRTRIGSPY
CCCCEECCCCCCEEECCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
VVAGMDASAADGVVVCYEANGGFLLESRVVHNGRTLEALPTRDSVLPILALLGMARERDE
EEEECCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHH
KVSGLSASLPKRFTASDRLKDVPTERSNSLISSLRDSPDEAARLLGPDAGGVAWMDMTDG
HHCCCCHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCC
LRMTFENGDIIHLRPSGNAPELRCYAEADSVERAEMLCAGALGAVTRDA
EEEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFLLGSGVGFGTSGARGLVVDMTDRVCYGFAAAFLGSVAGDAAGVVIGHDLRPSSPGIA
CCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
GACARAVMDSGRELVYAGALPTPALAFYAQRLGLPAIVVTGSHIPFDRNGIKFYRADGEI
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCC
SKADELAMMEAEVVVPEVVVPAVLPALDGRAREAYVRRYLDFFGAGSLEGMRVGVYEHSS
CCHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
VARDVLREILEGLGADVVSLGRTEVFVPIDTEAVRPEDVLQARAWADEVGFDAIVSTDGD
HHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
GDRPLIGDENGLWMRGDVVGILCAQFLRANVVVTPVSCNTAVEACGWFGKVVRTRIGSPY
CCCCEECCCCCCEEECCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCE
VVAGMDASAADGVVVCYEANGGFLLESRVVHNGRTLEALPTRDSVLPILALLGMARERDE
EEEECCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHH
KVSGLSASLPKRFTASDRLKDVPTERSNSLISSLRDSPDEAARLLGPDAGGVAWMDMTDG
HHCCCCHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCC
LRMTFENGDIIHLRPSGNAPELRCYAEADSVERAEMLCAGALGAVTRDA
EEEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]