Click here to switch to the map view.

The map label for this gene is greA

Identifier: 194334369

GI number: 194334369

Start: 1702469

End: 1702951

Strand: Reverse

Name: greA

Synonym: Paes_1564

Alternate gene names: 194334369

Gene position: 1702951-1702469 (Counterclockwise)

Preceding gene: 194334370

Following gene: 194334368

Centisome position: 67.77

GC content: 47.41

Gene sequence:

>483_bases
ATGAGTGACAGAATTTACCTGACCAAACAGGGATACAACAGAATGAGAGATGAACTCAATCTCTTGAAGACTGTGGTCAG
GAAGGAAGTTCTGGAAAAAATTGCTGAGGCTCGTGCTCATGGGGATTTGAGCGAAAATGCCGAGTATGAAGCAGCACGTG
AACAGCAGGCCCAGATGGAATCCAAGATCGTTGACCTGGAAAACAAGCTTACGCGCGCAAGTATTCTTGATCCGAAGCAG
ATCCGGACCGACAGGGTCTATATTCTCACATCCGTCAAGTTGCGAAACCTCGATGAGACGGACGATGAGATTATAGAGTA
CACCCTTGTTTCTTCGGAGGAGGCCGATACCGACCAGGGCAAGATTTCAGTTCGTTCTCCTGTAGGCAAGGCGCTGATCG
GAAAAGCCGTCGGCGAGAAAGTACAGATCCATGTGCCGAAAGGGGAGCTTCACTATGAGATTTTAGAGATATTTGTTAAA
TAA

Upstream 100 bases:

>100_bases
GTTTATGGTTATGAACATAGTTTGTGTTACGACAGGCCGTTTCCCAGGGGGAACGTCCTGCTTCTGTTCTTTTCTATCCT
AACAATTAAAGGAACGCGAT

Downstream 100 bases:

>100_bases
AAGCCGTTTTATTAACACGAAATGCTACAGGTATGGGAAAACGTCAGATGATATACCGATCCTCTGAAATTGCCGACAGT
GACGAACTGGTAGGAAAAGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSDRIYLTKQGYNRMRDELNLLKTVVRKEVLEKIAEARAHGDLSENAEYEAAREQQAQMESKIVDLENKLTRASILDPKQ
IRTDRVYILTSVKLRNLDETDDEIIEYTLVSSEEADTDQGKISVRSPVGKALIGKAVGEKVQIHVPKGELHYEILEIFVK

Sequences:

>Translated_160_residues
MSDRIYLTKQGYNRMRDELNLLKTVVRKEVLEKIAEARAHGDLSENAEYEAAREQQAQMESKIVDLENKLTRASILDPKQ
IRTDRVYILTSVKLRNLDETDDEIIEYTLVSSEEADTDQGKISVRSPVGKALIGKAVGEKVQIHVPKGELHYEILEIFVK
>Mature_159_residues
SDRIYLTKQGYNRMRDELNLLKTVVRKEVLEKIAEARAHGDLSENAEYEAAREQQAQMESKIVDLENKLTRASILDPKQI
RTDRVYILTSVKLRNLDETDDEIIEYTLVSSEEADTDQGKISVRSPVGKALIGKAVGEKVQIHVPKGELHYEILEIFVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=44.8051948051948, Blast_Score=122, Evalue=8e-30,
Organism=Escherichia coli, GI87082261, Length=149, Percent_Identity=28.1879194630872, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_PROA2 (B4S9B2)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016229.1
- GeneID:   6459320
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1564
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18284; Mature: 18153

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIYLTKQGYNRMRDELNLLKTVVRKEVLEKIAEARAHGDLSENAEYEAAREQQAQME
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
SKIVDLENKLTRASILDPKQIRTDRVYILTSVKLRNLDETDDEIIEYTLVSSEEADTDQG
HHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHEECCCCCCCCCC
KISVRSPVGKALIGKAVGEKVQIHVPKGELHYEILEIFVK
CEEECCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
SDRIYLTKQGYNRMRDELNLLKTVVRKEVLEKIAEARAHGDLSENAEYEAAREQQAQME
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
SKIVDLENKLTRASILDPKQIRTDRVYILTSVKLRNLDETDDEIIEYTLVSSEEADTDQG
HHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHEECCCCCCCCCC
KISVRSPVGKALIGKAVGEKVQIHVPKGELHYEILEIFVK
CEEECCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA