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The map label for this gene is panB

Identifier: 194334283

GI number: 194334283

Start: 1615464

End: 1616297

Strand: Reverse

Name: panB

Synonym: Paes_1476

Alternate gene names: 194334283

Gene position: 1616297-1615464 (Counterclockwise)

Preceding gene: 194334284

Following gene: 194334282

Centisome position: 64.32

GC content: 50.0

Gene sequence:

>834_bases
ATGAATAAGCAGAGCACGCAAAAACTGCCCCACGTCACAACACGACGTCTTCATGATATGAAATCAGAAGGTGAGAAAAT
TTCAATGCTCACTGCATATGATTATACAACGGCCAGAATTCTTGATCGCGCCGGTGTCGATGTTATTCTTGTTGGTGATT
CGGCAAGCAATGTTTTTGCCGGTCACAATACGACGCTGCCTATTACCATCGATCAGATGATCTACCATGCAAGAGCTGTT
GTCAGGGGAGTTCAGGCTGAAACCAGCAGAGCGATGGTTGTGATCGATATGCCGTTCATGAGTTATCAGCTCTCGAGCGA
AGAGGCTTTGCGCAATGCGGGTAAGATCATGAAGGAGAATGAGTGTGATGCTGTCAAAATCGAAGGGGGAAACGTTATTG
TCGATACGGTAAAGAGGATAACCGATGTCGGTATCCCGGTCATGGGACACCTGGGCCTGATTCCGCAGTCGATCTATAAG
TTCGGAAGCTATAAGGTGCGTGCACGAGAAGAACAGGAGGCCGAAGAGCTTCTTCGTGATGCGAAACTGCTTGAAGAGGC
AGGGGCCTTTGCTCTTGTTCTTGAAAAAATTCCGGCGGACCTTGCCGCAGAGGTGACCGCTTCGATCTCGATTCCAACGA
TAGGTATCGGTGCGGGGGGGGCGTGCGATGGTCAGGTTCTGGTTATCAACGATATGCTTGGTTTGAGTACCGAATTTCAT
CCCCGCTTCGTTCGCAGGTATGCTGAACTCGATCGTGTTATATTCGATGCCGTCAGCCGCTATGTCGATGATGTCCGGGG
CGGGACGTTTCCGGGGGAAGATGAAAGCTACTGA

Upstream 100 bases:

>100_bases
TATGACACAAGCCCTTGATGCAAGCTGGCTGACGGTCGCCAGGCCTCTTGATGTCCGGGATCTTGAAGCCCTATTTTGAT
CATTTTAACTCCTTGTTTGT

Downstream 100 bases:

>100_bases
ATCTTCTCTCCTTTTTGCCGACGTCATTTGTTTCAAAGATTTTGCAAAGATCGTACCTTTTATGAGTTACATCACACACC
AGCTCTCTTCGTGATACTTT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNKQSTQKLPHVTTRRLHDMKSEGEKISMLTAYDYTTARILDRAGVDVILVGDSASNVFAGHNTTLPITIDQMIYHARAV
VRGVQAETSRAMVVIDMPFMSYQLSSEEALRNAGKIMKENECDAVKIEGGNVIVDTVKRITDVGIPVMGHLGLIPQSIYK
FGSYKVRAREEQEAEELLRDAKLLEEAGAFALVLEKIPADLAAEVTASISIPTIGIGAGGACDGQVLVINDMLGLSTEFH
PRFVRRYAELDRVIFDAVSRYVDDVRGGTFPGEDESY

Sequences:

>Translated_277_residues
MNKQSTQKLPHVTTRRLHDMKSEGEKISMLTAYDYTTARILDRAGVDVILVGDSASNVFAGHNTTLPITIDQMIYHARAV
VRGVQAETSRAMVVIDMPFMSYQLSSEEALRNAGKIMKENECDAVKIEGGNVIVDTVKRITDVGIPVMGHLGLIPQSIYK
FGSYKVRAREEQEAEELLRDAKLLEEAGAFALVLEKIPADLAAEVTASISIPTIGIGAGGACDGQVLVINDMLGLSTEFH
PRFVRRYAELDRVIFDAVSRYVDDVRGGTFPGEDESY
>Mature_277_residues
MNKQSTQKLPHVTTRRLHDMKSEGEKISMLTAYDYTTARILDRAGVDVILVGDSASNVFAGHNTTLPITIDQMIYHARAV
VRGVQAETSRAMVVIDMPFMSYQLSSEEALRNAGKIMKENECDAVKIEGGNVIVDTVKRITDVGIPVMGHLGLIPQSIYK
FGSYKVRAREEQEAEELLRDAKLLEEAGAFALVLEKIPADLAAEVTASISIPTIGIGAGGACDGQVLVINDMLGLSTEFH
PRFVRRYAELDRVIFDAVSRYVDDVRGGTFPGEDESY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=41.1320754716981, Blast_Score=216, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319653, Length=255, Percent_Identity=36.078431372549, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PROA2 (B4S8V9)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016143.1
- ProteinModelPortal:   B4S8V9
- SMR:   B4S8V9
- GeneID:   6460457
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1476
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30395; Mature: 30395

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 195-195 BINDING 96-96 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQSTQKLPHVTTRRLHDMKSEGEKISMLTAYDYTTARILDRAGVDVILVGDSASNVFA
CCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCEEEEECCCCCCEEC
GHNTTLPITIDQMIYHARAVVRGVQAETSRAMVVIDMPFMSYQLSSEEALRNAGKIMKEN
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHEECCHHHHHHHHHHHCCCC
ECDAVKIEGGNVIVDTVKRITDVGIPVMGHLGLIPQSIYKFGSYKVRAREEQEAEELLRD
CCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHH
AKLLEEAGAFALVLEKIPADLAAEVTASISIPTIGIGAGGACDGQVLVINDMLGLSTEFH
HHHHHHCCHHHHHHHHCCHHHHHHHHHEEECCEEECCCCCCCCCEEEEEECCCCCCCCCC
PRFVRRYAELDRVIFDAVSRYVDDVRGGTFPGEDESY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNKQSTQKLPHVTTRRLHDMKSEGEKISMLTAYDYTTARILDRAGVDVILVGDSASNVFA
CCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCEEEEECCCCCCEEC
GHNTTLPITIDQMIYHARAVVRGVQAETSRAMVVIDMPFMSYQLSSEEALRNAGKIMKEN
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHEECCHHHHHHHHHHHCCCC
ECDAVKIEGGNVIVDTVKRITDVGIPVMGHLGLIPQSIYKFGSYKVRAREEQEAEELLRD
CCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHH
AKLLEEAGAFALVLEKIPADLAAEVTASISIPTIGIGAGGACDGQVLVINDMLGLSTEFH
HHHHHHCCHHHHHHHHCCHHHHHHHHHEEECCEEECCCCCCCCCEEEEEECCCCCCCCCC
PRFVRRYAELDRVIFDAVSRYVDDVRGGTFPGEDESY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA