Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 194334276

GI number: 194334276

Start: 1606991

End: 1608817

Strand: Reverse

Name: aspS

Synonym: Paes_1469

Alternate gene names: 194334276

Gene position: 1608817-1606991 (Counterclockwise)

Preceding gene: 194334277

Following gene: 194334275

Centisome position: 64.02

GC content: 51.23

Gene sequence:

>1827_bases
ATGAGCTTAGCGCCTGGAACTGAAACCGATTTACAGAATCGATTTCGTTCGCACTTTTGCGGACGTTTAAACACCGAATA
TGAAAATAAACAGGTCCGGCTTGCCGGCTGGATCCATAGAAAACGCGACCACGGAGGTTTGATCTTTATTGATCTTCGCG
ATCACACCGGCATTGCTCAGCTGATTATTCAGCCGGAGAAACAGGAGCTCTTCCAGAAAGTTGAGCGTCTTCATGTCGAG
TCGGTTATCGCTGTTCAAGGTACCGTCGTCAAGCGTTCGGAGGAGACGCTCAACTCCCGGATTCCATCAGGAGCCATCGA
GGTTCTCGTCGATGATGTCCAGGTTGAAAGCCATGCCGTTGCGCTGCCGTTCCCTGTTGCCGATGAACTTCAGACATCCG
AAGAACTTCGCCTGACCTATCGTTTTCTCGATTTGCGCAGAGAAAAGATTCATCAGAACATTGTTTTTCGCAGTCAGCTG
ATCAGCAAGGTACGCCGCTATCTCGAAGACCATGGGTTCATGGAGATCCAGACACCTATTCTTACAGCCAGTTCACCCGA
GGGTGCCAGGGATTTTCTTGTACCGAGTCGACTTCATCCCGGTAAATTTTACGCTCTTCCTCAGGCTCCGCAGCAGTTCA
AGCAGCTGTTGATGGTATCCGGTTTTCCTCGCTATTTCCAGATCGCTCCCTGCTTCCGCGATGAAGATGCTCGCGCCGAT
CGCAGCCCCGGTGAGTTCTATCAGGTCGATATGGAGATGGCATTTATCGAGCAGGATGACCTGTTCGAAATCCTTGAAGG
GATGTTGCGTTACCTTACCGAAACGATGAGCACGAAACGGATCACTCAGTTTCCGTTTCCGAGGCTCAGTTACCGCGATG
TCATGAACCGTTTTGGTTCCGATAAGCCTGATCTTCGTGTCCCGCTTGAAATGCAGGATGTTACCGAGCTCTTTGTTGGT
TCATCGTTCAAGGTGTTTGCGTCAAACACCAAAGAGGGAAGCTGCATCAAGGCAATGGTGCTTAAAGGCAGGGGAACCGA
GTCCCGTCAGTTTTACGATAAAGCAGAAAAGCGGGCCAGAGAGCTTGGTGCACCGGGACTTGCTTATGTCCAGTACAGAG
AAGAGGGGCCGAAAGGGCCGATCGTCAAGTTTCTTTCAGAGGCCGAGCTCAGTGCATTGCAGGAGCGTCTGGCTATAGAA
ACAGGCGACGTTGTTTTCTTCGGCGCCGGCAAGTGGGAAAAAACCTGCAAGATCATGGGCGGTATTCGGGAATACTTCTC
CGACCTCTTTGAGCTTGACCGCGATGAACTCTCTTTCTGCTGGATTGTCGACTTTCCTCTGTACGAATTCGATGAAAAAG
AGAGCCGGATCGACTTCTCGCACAATCCATTCTCAATGCCTCAGGGAGAGATGGATGCGCTCGATACCATGAACCCGCTT
GATATCCTTGCCTATCAGTATGATATCGTCTGTAACGGGATTGAGCTTTCCAGCGGCGCTATCCGTAACCATCGCCCCGA
TATCATGTACAGGGCATTTGAGATAGCAGGTTACAGCAAAGAGGAGGTTGACAAGCGTTTTGGGCACATGATCGAGGCGT
TTAAAATGGGAGCTCCTCCCCATGGTGGCATCGCTCCGGGTCTTGATCGTCTGGTTATGATCCTGCGCGATGAACACAAT
ATTCGTGAAGTTATCGCTTTTCCGATGAACCAGCAGGCTCAGGATCTGATGATGTCAGCGCCATCAGATGTTTCGCGCCT
GCAGCTGCGTGAGCTGCACCTTAAGCTCGACCTGCCGGTTGAAACGGCAGAGCAAGAGCCGGGGTGA

Upstream 100 bases:

>100_bases
CGAACTCGTGTATTGACGCGGGAAGAACTGTTTCCATAATCTGTAAAAATTCATCATATTCAGTGTTTTCGACACGTTTA
ACATCTCGTTACCACTATCT

Downstream 100 bases:

>100_bases
TGTATTGAGTTATTGATCTTCTTGAGCCGCAGGTTATTCCTGCGGCTTTTTTTTAGTGAACTCGGAGTTCCGTTCTCTTC
TGCTCCATGACTATCGGTTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSLAPGTETDLQNRFRSHFCGRLNTEYENKQVRLAGWIHRKRDHGGLIFIDLRDHTGIAQLIIQPEKQELFQKVERLHVE
SVIAVQGTVVKRSEETLNSRIPSGAIEVLVDDVQVESHAVALPFPVADELQTSEELRLTYRFLDLRREKIHQNIVFRSQL
ISKVRRYLEDHGFMEIQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARAD
RSPGEFYQVDMEMAFIEQDDLFEILEGMLRYLTETMSTKRITQFPFPRLSYRDVMNRFGSDKPDLRVPLEMQDVTELFVG
SSFKVFASNTKEGSCIKAMVLKGRGTESRQFYDKAEKRARELGAPGLAYVQYREEGPKGPIVKFLSEAELSALQERLAIE
TGDVVFFGAGKWEKTCKIMGGIREYFSDLFELDRDELSFCWIVDFPLYEFDEKESRIDFSHNPFSMPQGEMDALDTMNPL
DILAYQYDIVCNGIELSSGAIRNHRPDIMYRAFEIAGYSKEEVDKRFGHMIEAFKMGAPPHGGIAPGLDRLVMILRDEHN
IREVIAFPMNQQAQDLMMSAPSDVSRLQLRELHLKLDLPVETAEQEPG

Sequences:

>Translated_608_residues
MSLAPGTETDLQNRFRSHFCGRLNTEYENKQVRLAGWIHRKRDHGGLIFIDLRDHTGIAQLIIQPEKQELFQKVERLHVE
SVIAVQGTVVKRSEETLNSRIPSGAIEVLVDDVQVESHAVALPFPVADELQTSEELRLTYRFLDLRREKIHQNIVFRSQL
ISKVRRYLEDHGFMEIQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARAD
RSPGEFYQVDMEMAFIEQDDLFEILEGMLRYLTETMSTKRITQFPFPRLSYRDVMNRFGSDKPDLRVPLEMQDVTELFVG
SSFKVFASNTKEGSCIKAMVLKGRGTESRQFYDKAEKRARELGAPGLAYVQYREEGPKGPIVKFLSEAELSALQERLAIE
TGDVVFFGAGKWEKTCKIMGGIREYFSDLFELDRDELSFCWIVDFPLYEFDEKESRIDFSHNPFSMPQGEMDALDTMNPL
DILAYQYDIVCNGIELSSGAIRNHRPDIMYRAFEIAGYSKEEVDKRFGHMIEAFKMGAPPHGGIAPGLDRLVMILRDEHN
IREVIAFPMNQQAQDLMMSAPSDVSRLQLRELHLKLDLPVETAEQEPG
>Mature_607_residues
SLAPGTETDLQNRFRSHFCGRLNTEYENKQVRLAGWIHRKRDHGGLIFIDLRDHTGIAQLIIQPEKQELFQKVERLHVES
VIAVQGTVVKRSEETLNSRIPSGAIEVLVDDVQVESHAVALPFPVADELQTSEELRLTYRFLDLRREKIHQNIVFRSQLI
SKVRRYLEDHGFMEIQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARADR
SPGEFYQVDMEMAFIEQDDLFEILEGMLRYLTETMSTKRITQFPFPRLSYRDVMNRFGSDKPDLRVPLEMQDVTELFVGS
SFKVFASNTKEGSCIKAMVLKGRGTESRQFYDKAEKRARELGAPGLAYVQYREEGPKGPIVKFLSEAELSALQERLAIET
GDVVFFGAGKWEKTCKIMGGIREYFSDLFELDRDELSFCWIVDFPLYEFDEKESRIDFSHNPFSMPQGEMDALDTMNPLD
ILAYQYDIVCNGIELSSGAIRNHRPDIMYRAFEIAGYSKEEVDKRFGHMIEAFKMGAPPHGGIAPGLDRLVMILRDEHNI
REVIAFPMNQQAQDLMMSAPSDVSRLQLRELHLKLDLPVETAEQEPG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=36.6336633663366, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI45439306, Length=292, Percent_Identity=26.3698630136986, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI5031815, Length=266, Percent_Identity=30.0751879699248, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI194272210, Length=266, Percent_Identity=30.0751879699248, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI226491502, Length=259, Percent_Identity=23.1660231660232, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4758762, Length=250, Percent_Identity=27.6, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=41.0169491525424, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1790571, Length=315, Percent_Identity=27.3015873015873, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789256, Length=305, Percent_Identity=25.9016393442623, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787161, Length=280, Percent_Identity=24.2857142857143, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=32.8333333333333, Blast_Score=306, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=28.4172661870504, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=262, Percent_Identity=25.5725190839695, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=262, Percent_Identity=25.5725190839695, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=266, Percent_Identity=24.812030075188, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=629, Percent_Identity=30.0476947535771, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323011, Length=283, Percent_Identity=29.3286219081272, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=288, Percent_Identity=25.6944444444444, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=264, Percent_Identity=25.7575757575758, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=35.1575456053068, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=26.2295081967213, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921528, Length=291, Percent_Identity=25.4295532646048, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24640851, Length=285, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640849, Length=285, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PROA2 (B4S8V2)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016136.1
- ProteinModelPortal:   B4S8V2
- SMR:   B4S8V2
- GeneID:   6460558
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1469
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 69790; Mature: 69658

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAPGTETDLQNRFRSHFCGRLNTEYENKQVRLAGWIHRKRDHGGLIFIDLRDHTGIAQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCEEEEEECCCCCHHH
LIIQPEKQELFQKVERLHVESVIAVQGTVVKRSEETLNSRIPSGAIEVLVDDVQVESHAV
HEECCCHHHHHHHHHHHHHHHHHEECCEEEECCHHHHHHCCCCCHHHHEEEEEEECCCEE
ALPFPVADELQTSEELRLTYRFLDLRREKIHQNIVFRSQLISKVRRYLEDHGFMEIQTPI
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
LTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARAD
EECCCCCCCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
RSPGEFYQVDMEMAFIEQDDLFEILEGMLRYLTETMSTKRITQFPFPRLSYRDVMNRFGS
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
DKPDLRVPLEMQDVTELFVGSSFKVFASNTKEGSCIKAMVLKGRGTESRQFYDKAEKRAR
CCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
ELGAPGLAYVQYREEGPKGPIVKFLSEAELSALQERLAIETGDVVFFGAGKWEKTCKIMG
HHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEECCCEEEEECCCHHHHHHHHH
GIREYFSDLFELDRDELSFCWIVDFPLYEFDEKESRIDFSHNPFSMPQGEMDALDTMNPL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
DILAYQYDIVCNGIELSSGAIRNHRPDIMYRAFEIAGYSKEEVDKRFGHMIEAFKMGAPP
HHHHEEEEEEECCEEECCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGIAPGLDRLVMILRDEHNIREVIAFPMNQQAQDLMMSAPSDVSRLQLRELHLKLDLPV
CCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHEEEEECCC
ETAEQEPG
CCCCCCCC
>Mature Secondary Structure 
SLAPGTETDLQNRFRSHFCGRLNTEYENKQVRLAGWIHRKRDHGGLIFIDLRDHTGIAQ
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCEEEEEECCCCCHHH
LIIQPEKQELFQKVERLHVESVIAVQGTVVKRSEETLNSRIPSGAIEVLVDDVQVESHAV
HEECCCHHHHHHHHHHHHHHHHHEECCEEEECCHHHHHHCCCCCHHHHEEEEEEECCCEE
ALPFPVADELQTSEELRLTYRFLDLRREKIHQNIVFRSQLISKVRRYLEDHGFMEIQTPI
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
LTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARAD
EECCCCCCCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
RSPGEFYQVDMEMAFIEQDDLFEILEGMLRYLTETMSTKRITQFPFPRLSYRDVMNRFGS
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
DKPDLRVPLEMQDVTELFVGSSFKVFASNTKEGSCIKAMVLKGRGTESRQFYDKAEKRAR
CCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
ELGAPGLAYVQYREEGPKGPIVKFLSEAELSALQERLAIETGDVVFFGAGKWEKTCKIMG
HHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEECCCEEEEECCCHHHHHHHHH
GIREYFSDLFELDRDELSFCWIVDFPLYEFDEKESRIDFSHNPFSMPQGEMDALDTMNPL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHH
DILAYQYDIVCNGIELSSGAIRNHRPDIMYRAFEIAGYSKEEVDKRFGHMIEAFKMGAPP
HHHHEEEEEEECCEEECCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGIAPGLDRLVMILRDEHNIREVIAFPMNQQAQDLMMSAPSDVSRLQLRELHLKLDLPV
CCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHEEEEECCC
ETAEQEPG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA