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The map label for this gene is metG [H]

Identifier: 194334175

GI number: 194334175

Start: 1488513

End: 1490627

Strand: Reverse

Name: metG [H]

Synonym: Paes_1367

Alternate gene names: 194334175

Gene position: 1490627-1488513 (Counterclockwise)

Preceding gene: 194334176

Following gene: 194334173

Centisome position: 59.32

GC content: 50.35

Gene sequence:

>2115_bases
ATGAATCAGAATTTTAAGAGAACTCTTGTTACAACAGCGCTTCCCTATGCAAACGGACCGGTTCACCTCGGGCATCTGGC
CGGGGTCTATCTTCCTGCCGATATCTATGTCCGGTACCGGCGTCTGTGTGGAGATGACATTATTCATATAGGTGGTTCAG
ACGAGCATGGTGTTCCGATTACGATTACGGCCGACAGGGAGGGGGTGACTCCTCAGGATGTCGTTGACCGCTATCACGGT
ATGAACCTCGATGCTTTTACACGCTGCGGTATTTCATTCGATTATTACGGGCGCACCTCCTCTCGGCTTCATCATGCAAC
GGCACGTGAGTTTTTCCGGGAGATCAACGGCAAGAATATTTTTGTCAAAAAAACCGAAAAGCAATTTTTCGATCCTTCGG
CCGGGCGTTTTCTTTCCGATAGATATGTGACCGGAACCTGCCCCATCTGCAGCAGCCCTGACGCTAACGGTGATCAATGC
GAGCAGTGCGGAACACATCTCAGTCCCATGGAGCTTATTGATCCTAAAAGCAAGCTTTCCAACCAGGTTCCAGAGTTGCG
CGAGACTCTGCACTGGTATTTTCCTTTAGGAATTTTTCAGGAGCAGCTTGAAGGGTATGTCAACAGTCATCAGGATGACT
GGCGGTCGAATGTACTCAACTATACAAGAACCTGGTTGAAACAGGGGCTTACTGATCGTGCGATCACCCGCGACCTGTCG
TGGGGCGTGCCTGTGCCGGTTGATGGCCCTGAGGCCCTGGGGAAGGTTCTCTATGTCTGGTTCGATGCTGTTCTTGGTTA
CATCTCCTTTACGCGTCAGTGGGCTGAGGATCGTGGCAATCCTGAAGAATGGCGGCGTTACTGGCAGGATCCGGATTGCA
GGATCATCAATTTTATCGGCAAGGACAACGTTGTTTTTCATACACTGATGTTTCCTGCTCTTCTCATGGCCTGGAACCAG
GGGAGAGACGAGGAGGTCTATAACCTCGCCGATAATGTTCCTGCTTCCGAGTTCATGAACTTCGAGGGGCGCAAGTTTTC
CAAGTCAAGGAACTACGCAGTCTATCTCGGTGAGTTTCTCGACAGTTTCCCTGCCGATACGCTGCGTTATTGCATTGCTA
TGAACTATCCTGAAAACAAGGATAGCGATTTCAGCTGGACTGATTTTCAGAACCGGACGAACGGGGAACTGGCCGATACG
CTGGGAAACTTCATCAAGCGTTCGATCGATTTTTCCAATGTCCGTTTTGAGGGTGTTGTTCCCTGTGATTGCAGTATGGA
TGACTGGCAGGGTCTCGGGATAGACTGGCAAGACTCGTTTGCCTGTCTTGATGAAGCCTATCGGAATTTCCATTTCAGGG
AGGCTGCGAGCCTTTCCATGCAGATTGCACGCCGAGCAAACCGCTTTTTGACGGAGTCTGAACCATGGAAGGTGATTAAG
CAGGATCCTGAAAAGGCGGCAAAAACCATGGCACTGTCGCTCAACCTCTGCTACACGCTTGCCGTTGCCTTTTATCCGGT
TATTCCCTCTACTGCTTCCAGGATATATGCTATGCTTGGTTTTGATGAGAGTCTGGATGACTCGGTAGGCTCGGGTGCAG
TCAGATGGGATATGGCAAAAACCCCGCAGCTGAAGAAAGGTCATCGGCTTGGTGAGAAGTCGGACATTCTGTTTTCAAAA
ATCACTGACAGCGATATAGCGCCGGAGCTCGAGAAGATTGAAGCCCTTCTGGCTGGTTTGAAGGAACATGAAGCCGGACA
GCAGCTTCCTGATACTCCTCCTGTGAAGCCTGAGATCAGTTTTGACGATTTTCTCAAGGTGGACCTCCGGGTGGCCAGAG
TTGAGAGCTGTGAAAAAGTTAAAAAAGCAGATAAACTGCTGAAGCTGACCGTTTCGCTCGGCAGTCACACCCGTCAGGTA
CTGGCAGGTATTGCCAAATCCTATGCCCCTGAAGAGCTGATAGGGAAGCATGTCGTTATGGTAGCCAATCTTGCAGAGCG
TAAGATCCGCGGTGAGCTCTCGCAGGGAATGATTCTCGCAGTGGAAGGCTCTGATGGCATGTTACATGTCACTGAAACGT
CAGGAGATGGAATTATCGGCATGCCGGTGCAATAA

Upstream 100 bases:

>100_bases
TGGCGAGTTCAATACCATGTTCGTGAAAAAATAAGTTCTTCCCGGCCGGCAGTGCGCGTTTCAATGCCGGTTGTTAACAT
TTTCAAGCAGTGCTTCCCGT

Downstream 100 bases:

>100_bases
AGTTTGCAGTTTGTACTGATTTTTGTACATTGAAGCACAAAACATCGTGGGGTGGAGCAATTGGTAGCTCGTCGGGCTCA
TAACCCGAAGGTTGCAGGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MNQNFKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYRRLCGDDIIHIGGSDEHGVPITITADREGVTPQDVVDRYHG
MNLDAFTRCGISFDYYGRTSSRLHHATAREFFREINGKNIFVKKTEKQFFDPSAGRFLSDRYVTGTCPICSSPDANGDQC
EQCGTHLSPMELIDPKSKLSNQVPELRETLHWYFPLGIFQEQLEGYVNSHQDDWRSNVLNYTRTWLKQGLTDRAITRDLS
WGVPVPVDGPEALGKVLYVWFDAVLGYISFTRQWAEDRGNPEEWRRYWQDPDCRIINFIGKDNVVFHTLMFPALLMAWNQ
GRDEEVYNLADNVPASEFMNFEGRKFSKSRNYAVYLGEFLDSFPADTLRYCIAMNYPENKDSDFSWTDFQNRTNGELADT
LGNFIKRSIDFSNVRFEGVVPCDCSMDDWQGLGIDWQDSFACLDEAYRNFHFREAASLSMQIARRANRFLTESEPWKVIK
QDPEKAAKTMALSLNLCYTLAVAFYPVIPSTASRIYAMLGFDESLDDSVGSGAVRWDMAKTPQLKKGHRLGEKSDILFSK
ITDSDIAPELEKIEALLAGLKEHEAGQQLPDTPPVKPEISFDDFLKVDLRVARVESCEKVKKADKLLKLTVSLGSHTRQV
LAGIAKSYAPEELIGKHVVMVANLAERKIRGELSQGMILAVEGSDGMLHVTETSGDGIIGMPVQ

Sequences:

>Translated_704_residues
MNQNFKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYRRLCGDDIIHIGGSDEHGVPITITADREGVTPQDVVDRYHG
MNLDAFTRCGISFDYYGRTSSRLHHATAREFFREINGKNIFVKKTEKQFFDPSAGRFLSDRYVTGTCPICSSPDANGDQC
EQCGTHLSPMELIDPKSKLSNQVPELRETLHWYFPLGIFQEQLEGYVNSHQDDWRSNVLNYTRTWLKQGLTDRAITRDLS
WGVPVPVDGPEALGKVLYVWFDAVLGYISFTRQWAEDRGNPEEWRRYWQDPDCRIINFIGKDNVVFHTLMFPALLMAWNQ
GRDEEVYNLADNVPASEFMNFEGRKFSKSRNYAVYLGEFLDSFPADTLRYCIAMNYPENKDSDFSWTDFQNRTNGELADT
LGNFIKRSIDFSNVRFEGVVPCDCSMDDWQGLGIDWQDSFACLDEAYRNFHFREAASLSMQIARRANRFLTESEPWKVIK
QDPEKAAKTMALSLNLCYTLAVAFYPVIPSTASRIYAMLGFDESLDDSVGSGAVRWDMAKTPQLKKGHRLGEKSDILFSK
ITDSDIAPELEKIEALLAGLKEHEAGQQLPDTPPVKPEISFDDFLKVDLRVARVESCEKVKKADKLLKLTVSLGSHTRQV
LAGIAKSYAPEELIGKHVVMVANLAERKIRGELSQGMILAVEGSDGMLHVTETSGDGIIGMPVQ
>Mature_704_residues
MNQNFKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYRRLCGDDIIHIGGSDEHGVPITITADREGVTPQDVVDRYHG
MNLDAFTRCGISFDYYGRTSSRLHHATAREFFREINGKNIFVKKTEKQFFDPSAGRFLSDRYVTGTCPICSSPDANGDQC
EQCGTHLSPMELIDPKSKLSNQVPELRETLHWYFPLGIFQEQLEGYVNSHQDDWRSNVLNYTRTWLKQGLTDRAITRDLS
WGVPVPVDGPEALGKVLYVWFDAVLGYISFTRQWAEDRGNPEEWRRYWQDPDCRIINFIGKDNVVFHTLMFPALLMAWNQ
GRDEEVYNLADNVPASEFMNFEGRKFSKSRNYAVYLGEFLDSFPADTLRYCIAMNYPENKDSDFSWTDFQNRTNGELADT
LGNFIKRSIDFSNVRFEGVVPCDCSMDDWQGLGIDWQDSFACLDEAYRNFHFREAASLSMQIARRANRFLTESEPWKVIK
QDPEKAAKTMALSLNLCYTLAVAFYPVIPSTASRIYAMLGFDESLDDSVGSGAVRWDMAKTPQLKKGHRLGEKSDILFSK
ITDSDIAPELEKIEALLAGLKEHEAGQQLPDTPPVKPEISFDDFLKVDLRVARVESCEKVKKADKLLKLTVSLGSHTRQV
LAGIAKSYAPEELIGKHVVMVANLAERKIRGELSQGMILAVEGSDGMLHVTETSGDGIIGMPVQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=568, Percent_Identity=34.1549295774648, Blast_Score=342, Evalue=8e-94,
Organism=Homo sapiens, GI24308436, Length=584, Percent_Identity=24.486301369863, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788432, Length=691, Percent_Identity=34.5875542691751, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1789455, Length=88, Percent_Identity=36.3636363636364, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540794, Length=573, Percent_Identity=33.3333333333333, Blast_Score=358, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI212645096, Length=537, Percent_Identity=24.5810055865922, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=364, Percent_Identity=25.8241758241758, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321704, Length=570, Percent_Identity=35.0877192982456, Blast_Score=357, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655311, Length=577, Percent_Identity=33.6221837088388, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI21358483, Length=595, Percent_Identity=24.3697478991597, Blast_Score=125, Evalue=1e-28,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79592; Mature: 79592

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNFKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYRRLCGDDIIHIGGSDEHGVPI
CCCCHHHHHHHHCCCCCCCCEEEHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCCCEEE
TITADREGVTPQDVVDRYHGMNLDAFTRCGISFDYYGRTSSRLHHATAREFFREINGKNI
EEECCCCCCCHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEE
FVKKTEKQFFDPSAGRFLSDRYVTGTCPICSSPDANGDQCEQCGTHLSPMELIDPKSKLS
EEEECHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHCCCHHHHH
NQVPELRETLHWYFPLGIFQEQLEGYVNSHQDDWRSNVLNYTRTWLKQGLTDRAITRDLS
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
WGVPVPVDGPEALGKVLYVWFDAVLGYISFTRQWAEDRGNPEEWRRYWQDPDCRIINFIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEC
KDNVVFHTLMFPALLMAWNQGRDEEVYNLADNVPASEFMNFEGRKFSKSRNYAVYLGEFL
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCCEEEEHHHHH
DSFPADTLRYCIAMNYPENKDSDFSWTDFQNRTNGELADTLGNFIKRSIDFSNVRFEGVV
HHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCEE
PCDCSMDDWQGLGIDWQDSFACLDEAYRNFHFREAASLSMQIARRANRFLTESEPWKVIK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QDPEKAAKTMALSLNLCYTLAVAFYPVIPSTASRIYAMLGFDESLDDSVGSGAVRWDMAK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
TPQLKKGHRLGEKSDILFSKITDSDIAPELEKIEALLAGLKEHEAGQQLPDTPPVKPEIS
CCCCHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FDDFLKVDLRVARVESCEKVKKADKLLKLTVSLGSHTRQVLAGIAKSYAPEELIGKHVVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCHHHH
VANLAERKIRGELSQGMILAVEGSDGMLHVTETSGDGIIGMPVQ
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEEECCCC
>Mature Secondary Structure
MNQNFKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYRRLCGDDIIHIGGSDEHGVPI
CCCCHHHHHHHHCCCCCCCCEEEHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCCCEEE
TITADREGVTPQDVVDRYHGMNLDAFTRCGISFDYYGRTSSRLHHATAREFFREINGKNI
EEECCCCCCCHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEE
FVKKTEKQFFDPSAGRFLSDRYVTGTCPICSSPDANGDQCEQCGTHLSPMELIDPKSKLS
EEEECHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHCCCHHHHH
NQVPELRETLHWYFPLGIFQEQLEGYVNSHQDDWRSNVLNYTRTWLKQGLTDRAITRDLS
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
WGVPVPVDGPEALGKVLYVWFDAVLGYISFTRQWAEDRGNPEEWRRYWQDPDCRIINFIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEC
KDNVVFHTLMFPALLMAWNQGRDEEVYNLADNVPASEFMNFEGRKFSKSRNYAVYLGEFL
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCCEEEEHHHHH
DSFPADTLRYCIAMNYPENKDSDFSWTDFQNRTNGELADTLGNFIKRSIDFSNVRFEGVV
HHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCEE
PCDCSMDDWQGLGIDWQDSFACLDEAYRNFHFREAASLSMQIARRANRFLTESEPWKVIK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QDPEKAAKTMALSLNLCYTLAVAFYPVIPSTASRIYAMLGFDESLDDSVGSGAVRWDMAK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
TPQLKKGHRLGEKSDILFSKITDSDIAPELEKIEALLAGLKEHEAGQQLPDTPPVKPEIS
CCCCHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FDDFLKVDLRVARVESCEKVKKADKLLKLTVSLGSHTRQVLAGIAKSYAPEELIGKHVVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCHHHH
VANLAERKIRGELSQGMILAVEGSDGMLHVTETSGDGIIGMPVQ
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA