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The map label for this gene is dnaK [H]

Identifier: 194334135

GI number: 194334135

Start: 1442649

End: 1444268

Strand: Reverse

Name: dnaK [H]

Synonym: Paes_1323

Alternate gene names: 194334135

Gene position: 1444268-1442649 (Counterclockwise)

Preceding gene: 194334136

Following gene: 194334134

Centisome position: 57.47

GC content: 60.8

Gene sequence:

>1620_bases
ATGGCTCTTGCTTTTGATTTCGGTACCTGTAACAGTGTTGTCGCCCGCTGGAACGAGGCCCTGAATGATATTGAAACCCC
CGAGCTTCCCAACCTCGGCATCTCCTATCCCCAGCCCGGTGAGGCGGATGCGAGGGTCATTCCCTCCATGATTCATTTCG
GCGAACGTGACGCGCTGCTGGTCGGCAGCCAGGTTGCCGGGGCGGGCCTTGTCAACCATCCGGGAACCTTCAGGTGGCTG
AAGATGGATATGCTGCGTACGGGGGGAGCCAATCGCGGACGGCGCGTGAATGGCAGGGTGATCTATCCCCGCCTGGCGGC
GGACGATCTTGTGGATCGCATTCTGATGTATGTGCGCGGCGCGTTCGGCGACATTGACGACGAGCTTGTGCTGACCGTGC
CGGTCGAGGCGTTCGATCACTACATCGACTGGCTGCGAGAAGCGGCCATGAAGCGGTTTTCTGGCGGGATCAGCATCATT
GACGAGGCAACCGCCTGTATGCTTGGCTACAGCGACGCGGTGCTCGACAATGAGCCCTACTGCATCGTGGATTTCGGAGG
CGGCACGCTTGATGTTTCAATCGTCCGTACCGACCTGCGCAGTGCCTCTCATGCACGGTGCCGGATCCTCGGACGGGCGG
GCGAGGAGATCGGCGGCGTCATGATCGACAACTGGCTGCTGGAGCATATCCAGCAGGAGCAGGGGCTCGACGACGAGGAT
ATCGCCTCGATCGGTTCATCCCTGCTTGAAGCGGTCGAGCAGGCCAAAATTTCCATCGCAAACGGCGATCCGGAGGCATC
CTTCACCCGCTTTAACGATGTGACCGGCCGGCTGATAGAGGTGGCGCTTACAGCTGACACACTTAAAGAGGTGCTTGAGA
AAAAGCGCGAGCCGGGCATGAACAACCTCTACCAGCACATCGTCCGCACGCTTGACCGCGCGCTGGATCAGGCGCGCGAC
CGAGCGGGCATACGCAAGGAGGATCTGAAAGGGGTTTTCCTCGTTGGCGGGAGCAGCCTCCTGCCGGGCATCGAGGAGAA
AATCCGGGAGTTCTTTCCGCGGAGTGACGTCCATGCGGGCAATCCGTTCGAGGCCATCGCGAGGGGAGCGTGCCTCTTTT
CGGGTGGCATGATCGACCAGACGCTGACCCACGATTACTGCCTGCGAAGCTGGAACCGCGAACTCCGGGAGTTCGAGCTG
GTGCCTGTCGTGCCGCGCGGCACCCCGTACCCGACAGGCAAACCGGTGTGCAGCAAGTACATCAAGGCTGCCTCGGATGT
ACAGGAGGTGCTTGGACTGGTGATTTACGAACGCACGCTGATGAGCCGTCCCCTTATCTCCTACGTCAACGGTGCGGACG
GGCTTCGTCCCGTAAAGGAGGGAGAGCGTCTGGAGAGTCGTTCGAAGCCTCTCAATCCCGAAGACAGCGAATTTATTCAC
GCGGTGCCGCCGTGCCGTTCGGGCGACCGACGATTCATCGCCGGATTCGGGGTGGACAAGAGCCGCCGTCTGACGCTCTG
GCTGCGGGATACGGAACCGGACAACCGGTCCAGTATCCGGTTGCGAGATGGCTCCAGTCTTCCGCTTCCGGTGGCTGACT
TGCCGGTGGTGAAACTTTGA

Upstream 100 bases:

>100_bases
TCAGATTCGCTTCGTCGGTTTCCGGTTCAACGGGAAACTTATCGCAAAGGCTCAGGCAGGAGCGGTACAGGTCAAAACAT
GATATTTTAAAGGTATAGCC

Downstream 100 bases:

>100_bases
TCAAATTTATAGTACGTACTATGTCCTGGCTCGAAGAACTCAAACTAAACGTTGCGGCGCGTGTTGCCGTTATTCATCTG
GTGACCATCGATGAAGAGGA

Product: DnaK-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MALAFDFGTCNSVVARWNEALNDIETPELPNLGISYPQPGEADARVIPSMIHFGERDALLVGSQVAGAGLVNHPGTFRWL
KMDMLRTGGANRGRRVNGRVIYPRLAADDLVDRILMYVRGAFGDIDDELVLTVPVEAFDHYIDWLREAAMKRFSGGISII
DEATACMLGYSDAVLDNEPYCIVDFGGGTLDVSIVRTDLRSASHARCRILGRAGEEIGGVMIDNWLLEHIQQEQGLDDED
IASIGSSLLEAVEQAKISIANGDPEASFTRFNDVTGRLIEVALTADTLKEVLEKKREPGMNNLYQHIVRTLDRALDQARD
RAGIRKEDLKGVFLVGGSSLLPGIEEKIREFFPRSDVHAGNPFEAIARGACLFSGGMIDQTLTHDYCLRSWNRELREFEL
VPVVPRGTPYPTGKPVCSKYIKAASDVQEVLGLVIYERTLMSRPLISYVNGADGLRPVKEGERLESRSKPLNPEDSEFIH
AVPPCRSGDRRFIAGFGVDKSRRLTLWLRDTEPDNRSSIRLRDGSSLPLPVADLPVVKL

Sequences:

>Translated_539_residues
MALAFDFGTCNSVVARWNEALNDIETPELPNLGISYPQPGEADARVIPSMIHFGERDALLVGSQVAGAGLVNHPGTFRWL
KMDMLRTGGANRGRRVNGRVIYPRLAADDLVDRILMYVRGAFGDIDDELVLTVPVEAFDHYIDWLREAAMKRFSGGISII
DEATACMLGYSDAVLDNEPYCIVDFGGGTLDVSIVRTDLRSASHARCRILGRAGEEIGGVMIDNWLLEHIQQEQGLDDED
IASIGSSLLEAVEQAKISIANGDPEASFTRFNDVTGRLIEVALTADTLKEVLEKKREPGMNNLYQHIVRTLDRALDQARD
RAGIRKEDLKGVFLVGGSSLLPGIEEKIREFFPRSDVHAGNPFEAIARGACLFSGGMIDQTLTHDYCLRSWNRELREFEL
VPVVPRGTPYPTGKPVCSKYIKAASDVQEVLGLVIYERTLMSRPLISYVNGADGLRPVKEGERLESRSKPLNPEDSEFIH
AVPPCRSGDRRFIAGFGVDKSRRLTLWLRDTEPDNRSSIRLRDGSSLPLPVADLPVVKL
>Mature_538_residues
ALAFDFGTCNSVVARWNEALNDIETPELPNLGISYPQPGEADARVIPSMIHFGERDALLVGSQVAGAGLVNHPGTFRWLK
MDMLRTGGANRGRRVNGRVIYPRLAADDLVDRILMYVRGAFGDIDDELVLTVPVEAFDHYIDWLREAAMKRFSGGISIID
EATACMLGYSDAVLDNEPYCIVDFGGGTLDVSIVRTDLRSASHARCRILGRAGEEIGGVMIDNWLLEHIQQEQGLDDEDI
ASIGSSLLEAVEQAKISIANGDPEASFTRFNDVTGRLIEVALTADTLKEVLEKKREPGMNNLYQHIVRTLDRALDQARDR
AGIRKEDLKGVFLVGGSSLLPGIEEKIREFFPRSDVHAGNPFEAIARGACLFSGGMIDQTLTHDYCLRSWNRELREFELV
PVVPRGTPYPTGKPVCSKYIKAASDVQEVLGLVIYERTLMSRPLISYVNGADGLRPVKEGERLESRSKPLNPEDSEFIHA
VPPCRSGDRRFIAGFGVDKSRRLTLWLRDTEPDNRSSIRLRDGSSLPLPVADLPVVKL

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI16507237, Length=336, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI24234688, Length=463, Percent_Identity=22.4622030237581, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1786196, Length=507, Percent_Identity=24.8520710059172, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788875, Length=429, Percent_Identity=23.3100233100233, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786870, Length=432, Percent_Identity=23.1481481481481, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17534771, Length=330, Percent_Identity=26.0606060606061, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507981, Length=410, Percent_Identity=26.0975609756098, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562024, Length=473, Percent_Identity=22.4101479915433, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568549, Length=321, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322505, Length=443, Percent_Identity=23.9277652370203, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322426, Length=328, Percent_Identity=25, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324120, Length=342, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319972, Length=342, Percent_Identity=26.0233918128655, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320805, Length=325, Percent_Identity=26.7692307692308, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323401, Length=454, Percent_Identity=22.2466960352423, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641406, Length=414, Percent_Identity=23.4299516908213, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641408, Length=414, Percent_Identity=23.4299516908213, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641402, Length=414, Percent_Identity=23.4299516908213, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641404, Length=414, Percent_Identity=23.4299516908213, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653595, Length=468, Percent_Identity=22.6495726495727, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571678, Length=353, Percent_Identity=24.929178470255, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646324, Length=353, Percent_Identity=25.4957507082153, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646322, Length=353, Percent_Identity=25.4957507082153, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646318, Length=353, Percent_Identity=25.4957507082153, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 59602; Mature: 59470

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAFDFGTCNSVVARWNEALNDIETPELPNLGISYPQPGEADARVIPSMIHFGERDALL
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
VGSQVAGAGLVNHPGTFRWLKMDMLRTGGANRGRRVNGRVIYPRLAADDLVDRILMYVRG
ECCCCCCCCCCCCCCCCHHEEHHHHHCCCCCCCCEECCEEEECCHHHHHHHHHHHHHHHH
AFGDIDDELVLTVPVEAFDHYIDWLREAAMKRFSGGISIIDEATACMLGYSDAVLDNEPY
HCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHCCCCCE
CIVDFGGGTLDVSIVRTDLRSASHARCRILGRAGEEIGGVMIDNWLLEHIQQEQGLDDED
EEEECCCCEEEEEEEEHHHHCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCHHH
IASIGSSLLEAVEQAKISIANGDPEASFTRFNDVTGRLIEVALTADTLKEVLEKKREPGM
HHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCCHHEEEECHHHHHHHHHHHCCCCH
NNLYQHIVRTLDRALDQARDRAGIRKEDLKGVFLVGGSSLLPGIEEKIREFFPRSDVHAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHCCCHHHHHHHHCCCCCCCCC
NPFEAIARGACLFSGGMIDQTLTHDYCLRSWNRELREFELVPVVPRGTPYPTGKPVCSKY
CCHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHH
IKAASDVQEVLGLVIYERTLMSRPLISYVNGADGLRPVKEGERLESRSKPLNPEDSEFIH
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEE
AVPPCRSGDRRFIAGFGVDKSRRLTLWLRDTEPDNRSSIRLRDGSSLPLPVADLPVVKL
ECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALAFDFGTCNSVVARWNEALNDIETPELPNLGISYPQPGEADARVIPSMIHFGERDALL
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
VGSQVAGAGLVNHPGTFRWLKMDMLRTGGANRGRRVNGRVIYPRLAADDLVDRILMYVRG
ECCCCCCCCCCCCCCCCHHEEHHHHHCCCCCCCCEECCEEEECCHHHHHHHHHHHHHHHH
AFGDIDDELVLTVPVEAFDHYIDWLREAAMKRFSGGISIIDEATACMLGYSDAVLDNEPY
HCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHCCCCCE
CIVDFGGGTLDVSIVRTDLRSASHARCRILGRAGEEIGGVMIDNWLLEHIQQEQGLDDED
EEEECCCCEEEEEEEEHHHHCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCHHH
IASIGSSLLEAVEQAKISIANGDPEASFTRFNDVTGRLIEVALTADTLKEVLEKKREPGM
HHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCCHHEEEECHHHHHHHHHHHCCCCH
NNLYQHIVRTLDRALDQARDRAGIRKEDLKGVFLVGGSSLLPGIEEKIREFFPRSDVHAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHCCCHHHHHHHHCCCCCCCCC
NPFEAIARGACLFSGGMIDQTLTHDYCLRSWNRELREFELVPVVPRGTPYPTGKPVCSKY
CCHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHH
IKAASDVQEVLGLVIYERTLMSRPLISYVNGADGLRPVKEGERLESRSKPLNPEDSEFIH
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEE
AVPPCRSGDRRFIAGFGVDKSRRLTLWLRDTEPDNRSSIRLRDGSSLPLPVADLPVVKL
ECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA