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The map label for this gene is btuB [H]

Identifier: 194334090

GI number: 194334090

Start: 1390013

End: 1391728

Strand: Reverse

Name: btuB [H]

Synonym: Paes_1278

Alternate gene names: 194334090

Gene position: 1391728-1390013 (Counterclockwise)

Preceding gene: 194334091

Following gene: 194334089

Centisome position: 55.38

GC content: 53.61

Gene sequence:

>1716_bases
ATGAGTGTGTTATTGAGCGTGCTTTTCCTTGCCTTCTTGCTCGCTGTCGCGCCATCGGGGCTTTCCGCGCAGCAGAAGGA
CGAGAAGCTGGTGATCACGGCAGAGGAAATACGAACGATGAACGTGCAGAAAATCGACGACCTGCTCAACCGCATTCCTG
GAGTGAAGGCCGGTGATTCTTCTGTTTCCATTCGGGGTTCGTCGAATGTCAAGGTGATGCTCGACGGCCGTTCGATCAAC
GATCCTACTTCCCATTCCGGATCGGTGAAATGGTCGATGATTTCTCTTGAAGCCATTGAGCGCATCGTGGTGATAAAAGG
GGGTGGCAGTACCTCGTATGGCGACAATACCGGCGGCGGTGTTATCGTGATCACGTCCAGGCGTTCGGATCATTTCAGAG
GAACTGCAGGCGGGTACCTGGGTAACCATGGCCAGCAGAAAGCTACTCTCGATGTCCAAGGGGGGACAGAGCGCCTGTCG
GCAGGTTTTTCTGCAGGATATGAAAATTCTGACGGGTTCACCCTCAATGACGACAAGAGGCGCCGCCGTATGGGCGCGAG
GGCCGAGTACCGGTTTTCCGACGATTTCTCCCTTTTTCTCTCAGGGGATTACAATGACGAGCATAAAGGGTTGAGAGGCT
ATCCTGAACGGCGAACTCCGAATTCCAGGAAAGAGTATGACGATGTTTCCCTGCTTTTCGGCCAGGATGTCGGGGCTGTT
TCGGGAAAGACATGGTATTGTGTTGCCCGTACCCTCAGCACGGACCCGGACAGGGAGCTTTCAGCCTCTCTTGAGGTTAC
CAGGGCGGGCCAGAGTGTCGAGGGGCCGCTTTCTCTGCCCCTGGCAGGAAATGTCGACAGCGGTATAGGCTATGAATGGC
AGGAGGCGTCAGGTTCCAGCGTCGGGAAAGCCGATGAGGAACAGGCGTGGGTATTCATGTCGAAGACGATGCAGTTGGGC
AACGGCCCCTGGTTTGTGACGGCGGGTGCGAGAGGAAACATCTATTCCGAATATTCTGCTGCTGTCAATCCTGAAATGAA
GGCCCGTTACCGAACGAGCAAGCTTTCCGTCGAGCTTGCATACAGCCGGGCAAGCAATCTTCCTGCGTTCAGAAAACGGT
TTTACGAGTCGTCGGTGACAAAACCCAATCCCGATCTCCAGATGGAGAAAGCCACAAACTACGCTCTTTCACTTTCTGTC
GTTCCGGATTCTTCGCTCTCGTTCGATGCCTCGCTGTTTTACCGGGATATCAAGGACCGGATCACATACGTGAGAGATCT
CGAAACCAATACCGGACGGTACGAAAACTTCGGAGAAGTAACCTACAAGGGGGTCGAGGTTTCAGCGTCCTGGTCACCCT
CATCATTGTTTGAATTTACACCGTCTTACAGTTACCTGCATGCGCTGAACGAAGAGACAGGGTTCTGGCTTCCGGCAAAG
CCGTTTCATACCATCATGGCGGATGTGGTGTTCAGGCCGCTTGACGGGTGGTCCTTCAGGGCTGTCGTCAAGTATAACGG
GAAGACATATGTGGACTCACAGAATACCCGCACCCTGGCGGCGTATACCGTGGTCGATGCGAGAGCTGATTACAGCCTGG
GAGATCTGACTCTGTATTGCGACATCGATAACCTGCTTGACGAGAACTATTTCTATGTCGATGGCTACGATGCTCCTCCC
AGAGAGTGGGTCGTCGGCATGAACTATAACTTTTAG

Upstream 100 bases:

>100_bases
GTCGGGGCATGTGGAATGCGACGGAAGAGATGAAGCTGCGCCTGCAGGAGGAGTATCTGGAAACCGAAGGCGAGATCGAG
GAGATCAACGAGTGAAAAGA

Downstream 100 bases:

>100_bases
TGTCTATGCATAGCGTACTTTATCCGTTTTCAGCCGTTACAGGCCAGGAAGAGCTGAAACACGCACTGCTTCTCAACGCG
GTCAATCCCCGTATCGGCGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSVLLSVLFLAFLLAVAPSGLSAQQKDEKLVITAEEIRTMNVQKIDDLLNRIPGVKAGDSSVSIRGSSNVKVMLDGRSIN
DPTSHSGSVKWSMISLEAIERIVVIKGGGSTSYGDNTGGGVIVITSRRSDHFRGTAGGYLGNHGQQKATLDVQGGTERLS
AGFSAGYENSDGFTLNDDKRRRRMGARAEYRFSDDFSLFLSGDYNDEHKGLRGYPERRTPNSRKEYDDVSLLFGQDVGAV
SGKTWYCVARTLSTDPDRELSASLEVTRAGQSVEGPLSLPLAGNVDSGIGYEWQEASGSSVGKADEEQAWVFMSKTMQLG
NGPWFVTAGARGNIYSEYSAAVNPEMKARYRTSKLSVELAYSRASNLPAFRKRFYESSVTKPNPDLQMEKATNYALSLSV
VPDSSLSFDASLFYRDIKDRITYVRDLETNTGRYENFGEVTYKGVEVSASWSPSSLFEFTPSYSYLHALNEETGFWLPAK
PFHTIMADVVFRPLDGWSFRAVVKYNGKTYVDSQNTRTLAAYTVVDARADYSLGDLTLYCDIDNLLDENYFYVDGYDAPP
REWVVGMNYNF

Sequences:

>Translated_571_residues
MSVLLSVLFLAFLLAVAPSGLSAQQKDEKLVITAEEIRTMNVQKIDDLLNRIPGVKAGDSSVSIRGSSNVKVMLDGRSIN
DPTSHSGSVKWSMISLEAIERIVVIKGGGSTSYGDNTGGGVIVITSRRSDHFRGTAGGYLGNHGQQKATLDVQGGTERLS
AGFSAGYENSDGFTLNDDKRRRRMGARAEYRFSDDFSLFLSGDYNDEHKGLRGYPERRTPNSRKEYDDVSLLFGQDVGAV
SGKTWYCVARTLSTDPDRELSASLEVTRAGQSVEGPLSLPLAGNVDSGIGYEWQEASGSSVGKADEEQAWVFMSKTMQLG
NGPWFVTAGARGNIYSEYSAAVNPEMKARYRTSKLSVELAYSRASNLPAFRKRFYESSVTKPNPDLQMEKATNYALSLSV
VPDSSLSFDASLFYRDIKDRITYVRDLETNTGRYENFGEVTYKGVEVSASWSPSSLFEFTPSYSYLHALNEETGFWLPAK
PFHTIMADVVFRPLDGWSFRAVVKYNGKTYVDSQNTRTLAAYTVVDARADYSLGDLTLYCDIDNLLDENYFYVDGYDAPP
REWVVGMNYNF
>Mature_570_residues
SVLLSVLFLAFLLAVAPSGLSAQQKDEKLVITAEEIRTMNVQKIDDLLNRIPGVKAGDSSVSIRGSSNVKVMLDGRSIND
PTSHSGSVKWSMISLEAIERIVVIKGGGSTSYGDNTGGGVIVITSRRSDHFRGTAGGYLGNHGQQKATLDVQGGTERLSA
GFSAGYENSDGFTLNDDKRRRRMGARAEYRFSDDFSLFLSGDYNDEHKGLRGYPERRTPNSRKEYDDVSLLFGQDVGAVS
GKTWYCVARTLSTDPDRELSASLEVTRAGQSVEGPLSLPLAGNVDSGIGYEWQEASGSSVGKADEEQAWVFMSKTMQLGN
GPWFVTAGARGNIYSEYSAAVNPEMKARYRTSKLSVELAYSRASNLPAFRKRFYESSVTKPNPDLQMEKATNYALSLSVV
PDSSLSFDASLFYRDIKDRITYVRDLETNTGRYENFGEVTYKGVEVSASWSPSSLFEFTPSYSYLHALNEETGFWLPAKP
FHTIMADVVFRPLDGWSFRAVVKYNGKTYVDSQNTRTLAAYTVVDARADYSLGDLTLYCDIDNLLDENYFYVDGYDAPPR
EWVVGMNYNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=593, Percent_Identity=22.9342327150084, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 63159; Mature: 63028

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLLSVLFLAFLLAVAPSGLSAQQKDEKLVITAEEIRTMNVQKIDDLLNRIPGVKAGDS
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCCCC
SVSIRGSSNVKVMLDGRSINDPTSHSGSVKWSMISLEAIERIVVIKGGGSTSYGDNTGGG
CEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEHHHCCEEEEEECCCCCCCCCCCCCC
VIVITSRRSDHFRGTAGGYLGNHGQQKATLDVQGGTERLSAGFSAGYENSDGFTLNDDKR
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEECCHHH
RRRMGARAEYRFSDDFSLFLSGDYNDEHKGLRGYPERRTPNSRKEYDDVSLLFGQDVGAV
HHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
SGKTWYCVARTLSTDPDRELSASLEVTRAGQSVEGPLSLPLAGNVDSGIGYEWQEASGSS
CCCEEEEEEEECCCCCCCHHHCCEEEEECCCCCCCCEECCCCCCCCCCCCEEECCCCCCC
VGKADEEQAWVFMSKTMQLGNGPWFVTAGARGNIYSEYSAAVNPEMKARYRTSKLSVELA
CCCCCCCCEEEEEEEHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHEEEECEEEEEEE
YSRASNLPAFRKRFYESSVTKPNPDLQMEKATNYALSLSVVPDSSLSFDASLFYRDIKDR
ECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ITYVRDLETNTGRYENFGEVTYKGVEVSASWSPSSLFEFTPSYSYLHALNEETGFWLPAK
HHEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHEEECCCHHHHHHCCCCCCCCCCCC
PFHTIMADVVFRPLDGWSFRAVVKYNGKTYVDSQNTRTLAAYTVVDARADYSLGDLTLYC
HHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEE
DIDNLLDENYFYVDGYDAPPREWVVGMNYNF
EHHHHHCCCEEEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure 
SVLLSVLFLAFLLAVAPSGLSAQQKDEKLVITAEEIRTMNVQKIDDLLNRIPGVKAGDS
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCCCC
SVSIRGSSNVKVMLDGRSINDPTSHSGSVKWSMISLEAIERIVVIKGGGSTSYGDNTGGG
CEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEHHHCCEEEEEECCCCCCCCCCCCCC
VIVITSRRSDHFRGTAGGYLGNHGQQKATLDVQGGTERLSAGFSAGYENSDGFTLNDDKR
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEECCHHH
RRRMGARAEYRFSDDFSLFLSGDYNDEHKGLRGYPERRTPNSRKEYDDVSLLFGQDVGAV
HHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
SGKTWYCVARTLSTDPDRELSASLEVTRAGQSVEGPLSLPLAGNVDSGIGYEWQEASGSS
CCCEEEEEEEECCCCCCCHHHCCEEEEECCCCCCCCEECCCCCCCCCCCCEEECCCCCCC
VGKADEEQAWVFMSKTMQLGNGPWFVTAGARGNIYSEYSAAVNPEMKARYRTSKLSVELA
CCCCCCCCEEEEEEEHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHEEEECEEEEEEE
YSRASNLPAFRKRFYESSVTKPNPDLQMEKATNYALSLSVVPDSSLSFDASLFYRDIKDR
ECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ITYVRDLETNTGRYENFGEVTYKGVEVSASWSPSSLFEFTPSYSYLHALNEETGFWLPAK
HHEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHEEECCCHHHHHHCCCCCCCCCCCC
PFHTIMADVVFRPLDGWSFRAVVKYNGKTYVDSQNTRTLAAYTVVDARADYSLGDLTLYC
HHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEE
DIDNLLDENYFYVDGYDAPPREWVVGMNYNF
EHHHHHCCCEEEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA