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The map label for this gene is aslB [C]

Identifier: 194333949

GI number: 194333949

Start: 1236700

End: 1238061

Strand: Reverse

Name: aslB [C]

Synonym: Paes_1133

Alternate gene names: 194333949

Gene position: 1238061-1236700 (Counterclockwise)

Preceding gene: 194333950

Following gene: 194333948

Centisome position: 49.27

GC content: 48.38

Gene sequence:

>1362_bases
ATGGATCTGGACGAGACTTTTCGAATGTATCATCTTCGAAAAAAAGAACCTATGGCGGAAATTCACTACAGAACGACAAC
ATTCCGAATGCCGGAGCACATTCGCTATCTTGGCCACAATCGAAACGGACGCTCGGTATTTTTCAATCCGCTGGCTCCGG
ACTGGATAGCTGTAGAGGACGGAGTCGCGCTTGCTCTTTCCCTATGCGATGGTCATCGAAATATCGAAGAAATAACCAGA
ATTTTTGAACCCGGTACTTCTGACAAAATCACTATTGAACCAATGATGGAAACGCTGGCCTCTGCCGGGTGGCTCGAACC
GGCAACCGGCGATAACCATCAACCAACATCGAGGCAGAAGATGCCCGGAGCCCCACTTTCGTCGCTATCTCTCTACGTAA
CCGCATCCTGCAACCTTTCATGTCGCTACTGTTTTTACAATGCAGGCCACACGGATACCCCCGATGTATTGAAAACACAG
GACATCCTCAATCTTATTGACCGGATAACCGGCAGTACGGTATCGAACATGGTTTTTCTCGGAGGCGAACCCCTTCTGCA
CGACGATATTGCCCTGATCGGTCGTTACGCCCTTGACAGAGGCATTAAATCGCAACTTGTTACCAACGGCACTCTTATTA
ATGAAGAAAATTCCGAAGAGATCGCCGAATCCTTTGCTACGATTCAAATCAGTCTCGATGGTCTTAAAAAGGAACATGAG
GTAATACGGGGGAAAGCAAGTTTCGAACCGGCATTACGAGGCATGCGTCTGCTGCTGCACAAGAAATCGGATATTCGCGT
CAGTTGTATGGTCAGTCGTGTCAATGTTCATGAACTTGATGGTTTTATCAGCTTTCTATTCGAAGAGGGAGTCCGTCACG
TGCACTTCACCAGGCTGCTGATGACAGGAAGAGGACGTAGCGGCACAAATCATGCTATCAGTGAAAAAATATTTTACGAA
CTACTGGCTGGCCTGGAGGCAACATGGGGCTCGGTAATGCATATGGATCAGTACCGGGCGCTGTGCGACATCGTCAACAA
GCCCAAACACATCTGCGGCATCGGCAACGGAACTCTCGAAATCAATCATCTTGGGGACATCTACCCCTGTTATCGATTCA
TGGACGACGAATCGAAGCTTGGCAACATCAGAACGGATAGCCCACTCAGCCTTTATCAGGATTCAGATATCATTGCCAAA
CTCAGATGCGAAACCGTCGAGGCCGACGTCAATTGCCGTGACTGTCAGATCAGACTGCTTTGTGGGGGAGGGTGCCTTGC
CGATAGAAGCGAGAAGCTGGTAGATGCTGTAGAGTGCGATCAGAAAAGATCATTCTGGAATAATTTGATCCTTTCATCCT
AA

Upstream 100 bases:

>100_bases
GGCGTGTTATAAAGCGTATTAAAGATTAAAACACGACATCTTCATGATTGCCGGTATGATGTTTTGTTTTTCGGGGTGAT
CATGGGCCACTGTTAATCAG

Downstream 100 bases:

>100_bases
ACTGTCTGAAATAAAGTTTTATGTTTTTTAACCGGCAATATCGTTCACCTTTAAAATGAATCATCATGAAAAATAATGAA
TCCTCAAAACTCGTAAAAAT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDLDETFRMYHLRKKEPMAEIHYRTTTFRMPEHIRYLGHNRNGRSVFFNPLAPDWIAVEDGVALALSLCDGHRNIEEITR
IFEPGTSDKITIEPMMETLASAGWLEPATGDNHQPTSRQKMPGAPLSSLSLYVTASCNLSCRYCFYNAGHTDTPDVLKTQ
DILNLIDRITGSTVSNMVFLGGEPLLHDDIALIGRYALDRGIKSQLVTNGTLINEENSEEIAESFATIQISLDGLKKEHE
VIRGKASFEPALRGMRLLLHKKSDIRVSCMVSRVNVHELDGFISFLFEEGVRHVHFTRLLMTGRGRSGTNHAISEKIFYE
LLAGLEATWGSVMHMDQYRALCDIVNKPKHICGIGNGTLEINHLGDIYPCYRFMDDESKLGNIRTDSPLSLYQDSDIIAK
LRCETVEADVNCRDCQIRLLCGGGCLADRSEKLVDAVECDQKRSFWNNLILSS

Sequences:

>Translated_453_residues
MDLDETFRMYHLRKKEPMAEIHYRTTTFRMPEHIRYLGHNRNGRSVFFNPLAPDWIAVEDGVALALSLCDGHRNIEEITR
IFEPGTSDKITIEPMMETLASAGWLEPATGDNHQPTSRQKMPGAPLSSLSLYVTASCNLSCRYCFYNAGHTDTPDVLKTQ
DILNLIDRITGSTVSNMVFLGGEPLLHDDIALIGRYALDRGIKSQLVTNGTLINEENSEEIAESFATIQISLDGLKKEHE
VIRGKASFEPALRGMRLLLHKKSDIRVSCMVSRVNVHELDGFISFLFEEGVRHVHFTRLLMTGRGRSGTNHAISEKIFYE
LLAGLEATWGSVMHMDQYRALCDIVNKPKHICGIGNGTLEINHLGDIYPCYRFMDDESKLGNIRTDSPLSLYQDSDIIAK
LRCETVEADVNCRDCQIRLLCGGGCLADRSEKLVDAVECDQKRSFWNNLILSS
>Mature_453_residues
MDLDETFRMYHLRKKEPMAEIHYRTTTFRMPEHIRYLGHNRNGRSVFFNPLAPDWIAVEDGVALALSLCDGHRNIEEITR
IFEPGTSDKITIEPMMETLASAGWLEPATGDNHQPTSRQKMPGAPLSSLSLYVTASCNLSCRYCFYNAGHTDTPDVLKTQ
DILNLIDRITGSTVSNMVFLGGEPLLHDDIALIGRYALDRGIKSQLVTNGTLINEENSEEIAESFATIQISLDGLKKEHE
VIRGKASFEPALRGMRLLLHKKSDIRVSCMVSRVNVHELDGFISFLFEEGVRHVHFTRLLMTGRGRSGTNHAISEKIFYE
LLAGLEATWGSVMHMDQYRALCDIVNKPKHICGIGNGTLEINHLGDIYPCYRFMDDESKLGNIRTDSPLSLYQDSDIIAK
LRCETVEADVNCRDCQIRLLCGGGCLADRSEKLVDAVECDQKRSFWNNLILSS

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=354, Percent_Identity=24.2937853107345, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 50961; Mature: 50961

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDETFRMYHLRKKEPMAEIHYRTTTFRMPEHIRYLGHNRNGRSVFFNPLAPDWIAVED
CCHHHHHHHHHHHCCCCHHHEEEEEEEECCHHHHHHHCCCCCCCEEEECCCCCCEEEECC
GVALALSLCDGHRNIEEITRIFEPGTSDKITIEPMMETLASAGWLEPATGDNHQPTSRQK
CCEEEEEHHCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MPGAPLSSLSLYVTASCNLSCRYCFYNAGHTDTPDVLKTQDILNLIDRITGSTVSNMVFL
CCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEE
GGEPLLHDDIALIGRYALDRGIKSQLVTNGTLINEENSEEIAESFATIQISLDGLKKEHE
CCCCCCHHHHHHHHHHHHHHCHHHHHCCCCEEECCCCHHHHHHHHEEEEEEECCCCHHHH
VIRGKASFEPALRGMRLLLHKKSDIRVSCMVSRVNVHELDGFISFLFEEGVRHVHFTRLL
HHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHH
MTGRGRSGTNHAISEKIFYELLAGLEATWGSVMHMDQYRALCDIVNKPKHICGIGNGTLE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEE
INHLGDIYPCYRFMDDESKLGNIRTDSPLSLYQDSDIIAKLRCETVEADVNCRDCQIRLL
ECCCCCHHHHHHHCCCHHHCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCCCEEEEEE
CGGGCLADRSEKLVDAVECDQKRSFWNNLILSS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLDETFRMYHLRKKEPMAEIHYRTTTFRMPEHIRYLGHNRNGRSVFFNPLAPDWIAVED
CCHHHHHHHHHHHCCCCHHHEEEEEEEECCHHHHHHHCCCCCCCEEEECCCCCCEEEECC
GVALALSLCDGHRNIEEITRIFEPGTSDKITIEPMMETLASAGWLEPATGDNHQPTSRQK
CCEEEEEHHCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MPGAPLSSLSLYVTASCNLSCRYCFYNAGHTDTPDVLKTQDILNLIDRITGSTVSNMVFL
CCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEE
GGEPLLHDDIALIGRYALDRGIKSQLVTNGTLINEENSEEIAESFATIQISLDGLKKEHE
CCCCCCHHHHHHHHHHHHHHCHHHHHCCCCEEECCCCHHHHHHHHEEEEEEECCCCHHHH
VIRGKASFEPALRGMRLLLHKKSDIRVSCMVSRVNVHELDGFISFLFEEGVRHVHFTRLL
HHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHH
MTGRGRSGTNHAISEKIFYELLAGLEATWGSVMHMDQYRALCDIVNKPKHICGIGNGTLE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEE
INHLGDIYPCYRFMDDESKLGNIRTDSPLSLYQDSDIIAKLRCETVEADVNCRDCQIRLL
ECCCCCHHHHHHHCCCHHHCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCCCEEEEEE
CGGGCLADRSEKLVDAVECDQKRSFWNNLILSS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA