Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 193213653

GI number: 193213653

Start: 2220057

End: 2222456

Strand: Direct

Name: topA [H]

Synonym: Cpar_2019

Alternate gene names: 193213653

Gene position: 2220057-2222456 (Clockwise)

Preceding gene: 193213642

Following gene: 193213658

Centisome position: 96.98

GC content: 59.33

Gene sequence:

>2400_bases
ATGGCTTCAAAAAGCGCTACTCCATCGGCCAAAAACAGGACGCTCATCGTTGTCGAGTCACCATCCAAGGCCAGAACCAT
CAACAAGTACCTGGGCGACAACTACACGGTTTACGCCTCGGTCGGCCACATCAAGGATTTGCCGAAAAAAGAGATCGGCA
TCGATTTCGACAACCACTACGAGCCCCGTTACGAGGTGATCGCCGGAAAGGAGAAGGTGGTGCGGCAGCTCAAGAAACTT
GCCGGAGAAGCCAACGAAGTGCTGATCGCCACTGACCCCGACCGCGAAGGCGAGGCGATTGCCTGGCACATCTCCAACGA
GATCGAGTTCGCCAAAAAACCGGTCTATCGCGTGCTCTTCAACGAAATCACCAAAAACGCCATCATCGAGTCGGTCAACA
ATCCGAAGCAGATCGACTACCGGCTCGTGCGTTCGCAGCAGACCCGGCAGGGGCTCGACAAGATCGTAGGCTACAAGATC
AGCCCGTTCCTGTGGAATGTGGTCTATCGCGGACTTTCGGCTGGCCGTGTACAGTCGGTAGCACTTCGGCTGATCTGCGA
GCGGGAGAACGAGATCGAGGCGTTCGAGCCGCAGGAGTACTGGACGATTTATGCCGACTTCACCACGGAATCTGGCGAAA
CCTTCCGCACGAAGCTCGTCAAGGTCAAAGGCAAGAAACCGGAGATCGGCTCCGGCGAACAGGCCGAAGCCATCGTATCA
GCGATCAGGGACCGCCTGTTCGCGGTGAGCGAGATCGTGCCAAAGGTGCTTCAGCGCAAGCAGCCACTGCCGTTCACCAC
CTCACTCTTGCAGCAGGCGGCATCCAACCAGCTCGGCTTCGGTTCGAAAAAGACGATGCGCGCCGCGCAGCAGCTTTACG
AAGGCATCGACCTCGGTTCGGAGGGCGCGACCGGCCTCATCACCTACATGCGTACCGACTCGACCCGCGTCAGCGGAGAA
GCGATCGCGCAGGCGCATGACTACATTTCGCAGCAGTTCGGCGAAGAGTACAAAGGGTTCGGCGGGCAGGGCAAGGCTGG
CAAGAACGCGCAGGACGCGCACGAAGCGATCCGCCCGACCTCGGTCTATCGCACGCCTGAATCGCTGCGCAAGCATCTAT
CCGCAGACCAGTTCAAGCTCTACGAGCTGATCTGGAAGCGCTTCGTGGCCTCGATGATGGCTCCGGCCAAAATCGAGCAG
ACGCGCGTTGACGTGGAAGATCACCAGAAGGAGTTCGTGTTCCGCGCCACCGGCAACAAGGTGCTCTTCCCCGGCTTTTT
CAAGGTCTATAACGATCAGCAGGAGCTCGAATACGAGGCGCAGAAATCGACCCGCGACGAGGTTGAAAAAGAGCAGATGG
TCAAGCTGCCCGAAAAGCTCTCGGTCGAAGAGCAACTCAGGATGTCGGAGCTCGACCGCAAGCAGAGCTTCACCCGTCCG
CCCGCCCGCTTCACCGAAGCAAGCCTGGTCAAAGAGCTGGACAACTACGGCATCGGCCGCCCGTCAACCTATGCGGCAAT
CTTCTCGACCCTGCAGGAGCGCCGCTACGTCGAGCTTCAGAAGAAGAAGATCGTGCCGACCGAGCTGGGTCGGGACGTTT
CGACCATCCTCGTGGCCAACTTCCCCGACCTGTTCAACGTGAAGTTCACCGCCGAAATGGAGGACGAGCTGGACAAGGTC
GCATCGGGAGATGATGAGTACGAAAAGGTGCTCGACAGCTTCTACCGTCCGCTCGAAACGGTGCTGAGCCACCGCAAAAG
CGATCCGCTCATTCCGCAGAACCGCGAAGCGGAACGGTGCGACAAGTGCGGCGAGGGACACATGATCGTCAAATGGACCA
GCAGCGGCAAGTTCCTCGGCTGCTCGAACTACCCGAAGTGCAAAAACATCAAGGCGCTCGCCACCAACAAGGCCAAACCG
AAAGAAACCGGCGTCAAATGCCCGGTGTGCGGCGAAGGACACATGCTATTGCGCAACGGCCGATTCGGCCCGTTCCTGGC
CTGTTCGAACTATCCGAAGTGCAACACCCTGCTCAACCTCAGCAAGCAGCGCCACATCGAACCGATCAAAACGCCACCGT
TCACCACCGACCTCGAATGCCCGAAGTGCGGCGCGCCGATGTACCTCAGAACCGGCAAGCGCGGCCTGTGGCTTGGCTGC
TCGAAATTCCCCAAATGCCGCGGACGGCTCTCGTGGAACTCGCTTGACGAAGCGACCCAAACGCGCCTTGAGAAGACCAT
GGACGAACATCTGGCCGCCCACCCCTCCGTGGCGATCACCATGCTCGACGGTACGCCCGCGCCGATGAATCTGCCCATCG
ACGACATCATCATCCGCGCAGAAGACGCCGGGCTCATCAACGCCCCGAGCGAAGAGGTGGAGAGCGAAGCCTCAGCGTAG

Upstream 100 bases:

>100_bases
CAACAGTTTTCGCGACGGGGCCAATACGGCGCCCGAAACGAGCGGCAGGTTGCAGCGATGCCCTCTGTCCAAATCCCAAC
GCAAAAGGGATCAAAACCCC

Downstream 100 bases:

>100_bases
GAGTTGGGACTGACACCGATAACGCAACTGAAGCAAAAGCCCGTCAAAAAAGTATCGCCATCGAATCCGATCATGATTCG
ATGGCGATGTTGCTTTCAGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MASKSATPSAKNRTLIVVESPSKARTINKYLGDNYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKL
AGEANEVLIATDPDREGEAIAWHISNEIEFAKKPVYRVLFNEITKNAIIESVNNPKQIDYRLVRSQQTRQGLDKIVGYKI
SPFLWNVVYRGLSAGRVQSVALRLICERENEIEAFEPQEYWTIYADFTTESGETFRTKLVKVKGKKPEIGSGEQAEAIVS
AIRDRLFAVSEIVPKVLQRKQPLPFTTSLLQQAASNQLGFGSKKTMRAAQQLYEGIDLGSEGATGLITYMRTDSTRVSGE
AIAQAHDYISQQFGEEYKGFGGQGKAGKNAQDAHEAIRPTSVYRTPESLRKHLSADQFKLYELIWKRFVASMMAPAKIEQ
TRVDVEDHQKEFVFRATGNKVLFPGFFKVYNDQQELEYEAQKSTRDEVEKEQMVKLPEKLSVEEQLRMSELDRKQSFTRP
PARFTEASLVKELDNYGIGRPSTYAAIFSTLQERRYVELQKKKIVPTELGRDVSTILVANFPDLFNVKFTAEMEDELDKV
ASGDDEYEKVLDSFYRPLETVLSHRKSDPLIPQNREAERCDKCGEGHMIVKWTSSGKFLGCSNYPKCKNIKALATNKAKP
KETGVKCPVCGEGHMLLRNGRFGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPFTTDLECPKCGAPMYLRTGKRGLWLGC
SKFPKCRGRLSWNSLDEATQTRLEKTMDEHLAAHPSVAITMLDGTPAPMNLPIDDIIIRAEDAGLINAPSEEVESEASA

Sequences:

>Translated_799_residues
MASKSATPSAKNRTLIVVESPSKARTINKYLGDNYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKL
AGEANEVLIATDPDREGEAIAWHISNEIEFAKKPVYRVLFNEITKNAIIESVNNPKQIDYRLVRSQQTRQGLDKIVGYKI
SPFLWNVVYRGLSAGRVQSVALRLICERENEIEAFEPQEYWTIYADFTTESGETFRTKLVKVKGKKPEIGSGEQAEAIVS
AIRDRLFAVSEIVPKVLQRKQPLPFTTSLLQQAASNQLGFGSKKTMRAAQQLYEGIDLGSEGATGLITYMRTDSTRVSGE
AIAQAHDYISQQFGEEYKGFGGQGKAGKNAQDAHEAIRPTSVYRTPESLRKHLSADQFKLYELIWKRFVASMMAPAKIEQ
TRVDVEDHQKEFVFRATGNKVLFPGFFKVYNDQQELEYEAQKSTRDEVEKEQMVKLPEKLSVEEQLRMSELDRKQSFTRP
PARFTEASLVKELDNYGIGRPSTYAAIFSTLQERRYVELQKKKIVPTELGRDVSTILVANFPDLFNVKFTAEMEDELDKV
ASGDDEYEKVLDSFYRPLETVLSHRKSDPLIPQNREAERCDKCGEGHMIVKWTSSGKFLGCSNYPKCKNIKALATNKAKP
KETGVKCPVCGEGHMLLRNGRFGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPFTTDLECPKCGAPMYLRTGKRGLWLGC
SKFPKCRGRLSWNSLDEATQTRLEKTMDEHLAAHPSVAITMLDGTPAPMNLPIDDIIIRAEDAGLINAPSEEVESEASA
>Mature_798_residues
ASKSATPSAKNRTLIVVESPSKARTINKYLGDNYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKLA
GEANEVLIATDPDREGEAIAWHISNEIEFAKKPVYRVLFNEITKNAIIESVNNPKQIDYRLVRSQQTRQGLDKIVGYKIS
PFLWNVVYRGLSAGRVQSVALRLICERENEIEAFEPQEYWTIYADFTTESGETFRTKLVKVKGKKPEIGSGEQAEAIVSA
IRDRLFAVSEIVPKVLQRKQPLPFTTSLLQQAASNQLGFGSKKTMRAAQQLYEGIDLGSEGATGLITYMRTDSTRVSGEA
IAQAHDYISQQFGEEYKGFGGQGKAGKNAQDAHEAIRPTSVYRTPESLRKHLSADQFKLYELIWKRFVASMMAPAKIEQT
RVDVEDHQKEFVFRATGNKVLFPGFFKVYNDQQELEYEAQKSTRDEVEKEQMVKLPEKLSVEEQLRMSELDRKQSFTRPP
ARFTEASLVKELDNYGIGRPSTYAAIFSTLQERRYVELQKKKIVPTELGRDVSTILVANFPDLFNVKFTAEMEDELDKVA
SGDDEYEKVLDSFYRPLETVLSHRKSDPLIPQNREAERCDKCGEGHMIVKWTSSGKFLGCSNYPKCKNIKALATNKAKPK
ETGVKCPVCGEGHMLLRNGRFGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPFTTDLECPKCGAPMYLRTGKRGLWLGCS
KFPKCRGRLSWNSLDEATQTRLEKTMDEHLAAHPSVAITMLDGTPAPMNLPIDDIIIRAEDAGLINAPSEEVESEASA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=718, Percent_Identity=22.9805013927577, Blast_Score=126, Evalue=8e-29,
Organism=Homo sapiens, GI10835218, Length=627, Percent_Identity=24.0829346092504, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1787529, Length=820, Percent_Identity=38.6585365853659, Blast_Score=487, Evalue=1e-138,
Organism=Escherichia coli, GI1788061, Length=646, Percent_Identity=22.2910216718266, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994931, Length=139, Percent_Identity=34.5323741007194, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563869, Length=761, Percent_Identity=24.8357424441524, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=539, Percent_Identity=23.9332096474954, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=665, Percent_Identity=24.0601503759398, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24640096, Length=707, Percent_Identity=23.1966053748232, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 90035; Mature: 89903

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKSATPSAKNRTLIVVESPSKARTINKYLGDNYTVYASVGHIKDLPKKEIGIDFDNHY
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLAGEANEVLIATDPDREGEAIAWHISNEIEFAKKPVYRVLF
CCCHHEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHH
NEITKNAIIESVNNPKQIDYRLVRSQQTRQGLDKIVGYKISPFLWNVVYRGLSAGRVQSV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ALRLICERENEIEAFEPQEYWTIYADFTTESGETFRTKLVKVKGKKPEIGSGEQAEAIVS
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
AIRDRLFAVSEIVPKVLQRKQPLPFTTSLLQQAASNQLGFGSKKTMRAAQQLYEGIDLGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EGATGLITYMRTDSTRVSGEAIAQAHDYISQQFGEEYKGFGGQGKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
SVYRTPESLRKHLSADQFKLYELIWKRFVASMMAPAKIEQTRVDVEDHQKEFVFRATGNK
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCEEEEEECCCE
VLFPGFFKVYNDQQELEYEAQKSTRDEVEKEQMVKLPEKLSVEEQLRMSELDRKQSFTRP
EECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCC
PARFTEASLVKELDNYGIGRPSTYAAIFSTLQERRYVELQKKKIVPTELGRDVSTILVAN
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEC
FPDLFNVKFTAEMEDELDKVASGDDEYEKVLDSFYRPLETVLSHRKSDPLIPQNREAERC
CCCHHEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
DKCGEGHMIVKWTSSGKFLGCSNYPKCKNIKALATNKAKPKETGVKCPVCGEGHMLLRNG
HHCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCEEEECC
RFGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPFTTDLECPKCGAPMYLRTGKRGLWLGC
CCCCEEEECCCCCHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECC
SKFPKCRGRLSWNSLDEATQTRLEKTMDEHLAAHPSVAITMLDGTPAPMNLPIDDIIIRA
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEEEEE
EDAGLINAPSEEVESEASA
CCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
ASKSATPSAKNRTLIVVESPSKARTINKYLGDNYTVYASVGHIKDLPKKEIGIDFDNHY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLAGEANEVLIATDPDREGEAIAWHISNEIEFAKKPVYRVLF
CCCHHEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHH
NEITKNAIIESVNNPKQIDYRLVRSQQTRQGLDKIVGYKISPFLWNVVYRGLSAGRVQSV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ALRLICERENEIEAFEPQEYWTIYADFTTESGETFRTKLVKVKGKKPEIGSGEQAEAIVS
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
AIRDRLFAVSEIVPKVLQRKQPLPFTTSLLQQAASNQLGFGSKKTMRAAQQLYEGIDLGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EGATGLITYMRTDSTRVSGEAIAQAHDYISQQFGEEYKGFGGQGKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
SVYRTPESLRKHLSADQFKLYELIWKRFVASMMAPAKIEQTRVDVEDHQKEFVFRATGNK
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCEEEEEECCCE
VLFPGFFKVYNDQQELEYEAQKSTRDEVEKEQMVKLPEKLSVEEQLRMSELDRKQSFTRP
EECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCC
PARFTEASLVKELDNYGIGRPSTYAAIFSTLQERRYVELQKKKIVPTELGRDVSTILVAN
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEC
FPDLFNVKFTAEMEDELDKVASGDDEYEKVLDSFYRPLETVLSHRKSDPLIPQNREAERC
CCCHHEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
DKCGEGHMIVKWTSSGKFLGCSNYPKCKNIKALATNKAKPKETGVKCPVCGEGHMLLRNG
HHCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCEEEECC
RFGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPFTTDLECPKCGAPMYLRTGKRGLWLGC
CCCCEEEECCCCCHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECC
SKFPKCRGRLSWNSLDEATQTRLEKTMDEHLAAHPSVAITMLDGTPAPMNLPIDDIIIRA
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEEEEE
EDAGLINAPSEEVESEASA
CCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]