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The map label for this gene is ruvC

Identifier: 193212195

GI number: 193212195

Start: 566038

End: 566601

Strand: Direct

Name: ruvC

Synonym: Cpar_0526

Alternate gene names: 193212195

Gene position: 566038-566601 (Clockwise)

Preceding gene: 193212194

Following gene: 193212196

Centisome position: 24.73

GC content: 62.41

Gene sequence:

>564_bases
ATGATTGTGCTGGGCGTTGATCCGGGCAGTCGCAAGACCGGCTATGGCGTGATCGCCGAAGCGGAGGGGCGTCTCTCGGT
GCTCGGTTGCGGGCTTATTCGTCCCGCCGCTTCCGGAACGCTGCACGAACGGATCGGCCAGCTCTGTTCAGGACTCGAAG
AGGTGATCGCAAACATGAAGCCCGAAGCGGTTGCGCTTGAAACCGCGTTTGTCGGGCGCAACGTCCGGAGCGCGCTGATT
CTCGGTCAGGTGCGGGGCGCGGTGCTCGCCACGGTGATAAAGCTCGGATTGCCCGTGCGTGAATATGCGCCGAGGGAGAT
CAAGCTTGCGGTGACCGGCACCGGCGCCGCGCGCAAAGAGCAGGTTGCGTCGATGTTGCCTCGGTTGCTGGGGCTCGAAG
AAGCGCCGAAGCCGCTTGATGTCACCGACGCGCTTGGCATCGCATATTGCGATCTTTCAAGAGGTGCTTCCCTGACTGGT
CACTGCGGCATGACCGGGAGCGGAAAAAGCCGAAGCAAGGGATGGGCAGCCTTCGTGGACGCACATCCTGAACTGTTGGC
GTGA

Upstream 100 bases:

>100_bases
GTGGTGCTCGAAATCCTCACGTACTATCTGTACGCTCCGGTTTCTGCGCTCCAGCCGCCTTGCACTCGAATCGCTCACGA
CACTTTTTAGAGGTGCCTAA

Downstream 100 bases:

>100_bases
TAGCGTATCATCGGTAAAAAGCGCAGTGTCGCATCTCACCCCCCGCGGTGTGGATTCGGCAACCAATTTCGTTCAGTGAA
GCTCAGGATGGCCTTCGGGC

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIVLGVDPGSRKTGYGVIAEAEGRLSVLGCGLIRPAASGTLHERIGQLCSGLEEVIANMKPEAVALETAFVGRNVRSALI
LGQVRGAVLATVIKLGLPVREYAPREIKLAVTGTGAARKEQVASMLPRLLGLEEAPKPLDVTDALGIAYCDLSRGASLTG
HCGMTGSGKSRSKGWAAFVDAHPELLA

Sequences:

>Translated_187_residues
MIVLGVDPGSRKTGYGVIAEAEGRLSVLGCGLIRPAASGTLHERIGQLCSGLEEVIANMKPEAVALETAFVGRNVRSALI
LGQVRGAVLATVIKLGLPVREYAPREIKLAVTGTGAARKEQVASMLPRLLGLEEAPKPLDVTDALGIAYCDLSRGASLTG
HCGMTGSGKSRSKGWAAFVDAHPELLA
>Mature_187_residues
MIVLGVDPGSRKTGYGVIAEAEGRLSVLGCGLIRPAASGTLHERIGQLCSGLEEVIANMKPEAVALETAFVGRNVRSALI
LGQVRGAVLATVIKLGLPVREYAPREIKLAVTGTGAARKEQVASMLPRLLGLEEAPKPLDVTDALGIAYCDLSRGASLTG
HCGMTGSGKSRSKGWAAFVDAHPELLA

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=136, Percent_Identity=43.3823529411765, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CHLP8 (B3QLZ5)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998148.1
- GeneID:   6419440
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0526
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   CLSK2303952
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19397; Mature: 19397

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLGVDPGSRKTGYGVIAEAEGRLSVLGCGLIRPAASGTLHERIGQLCSGLEEVIANMK
CEEEECCCCCCCCCCEEEEECCCCEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PEAVALETAFVGRNVRSALILGQVRGAVLATVIKLGLPVREYAPREIKLAVTGTGAARKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHH
QVASMLPRLLGLEEAPKPLDVTDALGIAYCDLSRGASLTGHCGMTGSGKSRSKGWAAFVD
HHHHHHHHHHCCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AHPELLA
CCHHHCC
>Mature Secondary Structure
MIVLGVDPGSRKTGYGVIAEAEGRLSVLGCGLIRPAASGTLHERIGQLCSGLEEVIANMK
CEEEECCCCCCCCCCEEEEECCCCEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PEAVALETAFVGRNVRSALILGQVRGAVLATVIKLGLPVREYAPREIKLAVTGTGAARKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHH
QVASMLPRLLGLEEAPKPLDVTDALGIAYCDLSRGASLTGHCGMTGSGKSRSKGWAAFVD
HHHHHHHHHHCCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AHPELLA
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA