Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 193212122

GI number: 193212122

Start: 484151

End: 486175

Strand: Direct

Name: ligA

Synonym: Cpar_0453

Alternate gene names: 193212122

Gene position: 484151-486175 (Clockwise)

Preceding gene: 193212121

Following gene: 193212123

Centisome position: 21.15

GC content: 63.46

Gene sequence:

>2025_bases
ATGGATAAAGCGAAAGCACAGCAGGAAATCGGCAAGCTTCGCGCAGAGATCGAGCGCCACAATCGCCTCTACTATCTCGA
AGCGAAACCGGAGATTTCTGACTTCGAGTTCGACAAGCTGCTCGAAAGGCTGATCGCGCTCGAAACGGAGTTCCCGGAAC
TCGTCACGCCCGACAGCCCGTCGCAGCGCGTCGGTGGTGGCATCACCAAAGAGTTTCCGACGGTCATCCACCGCGAGCCG
ATGCTTAGCCTCTCGAACACCTACTCTATCGGCGAGGTGTCGGACTTCTGCACCCGCGTCGAAAAGCTGGTGGCAGCCGA
GGGTGGCGGCACGCCCGAATACGTCGCCGAGCTGAAGTATGATGGCGTGGCGATCAGCCTGTTTTACCGCGACGGCCTGC
TCGTTCGCGGCTCCACGCGCGGCGACGGTCGGCAGGGCGACGAGATCACGGCCAATCTCAAGACGATTCCGTCGATTCCG
CTCCGGCTTCAATCTGCCGGTGGATCGCTGTTCGATGCAGCCGTCAACGGCGAAGTCGAGGTGCGCGGCGAGGTGTACAT
GCGCAAGGATGATTTCGAGCGGCTCAACGAGGAGCGCCCGGAGGAGGAGCGCTTCGCCAACCCGCGCAACGCTACCGCCG
GAACGCTCAAGTTGCAGGACTCGGCGGAGGTCGCCCGGCGCAGGATGTCGTTCGTCGCCTACTACCTCAAGGGGCACGAC
GGCGAAGCTCCGACGCACTTGAGGCGGTTGGAGCAGCTTAAATGCATGGGCTTCATGACTGGCGCGGCGGCCCGGCTCTG
CAAGGGGATGGACGAGATTGCCAACTTCATCGGTGAGTGGTCGGAGAAGCGCTGGACGCTGCCGTACGAAACCGACGGCG
TGGTGCTCAAACTCAACGAGGTTGGCTTGTGGGATCGGCTCGGCGCGACCGCCAAAAGCCCGCGCTGGGCGATTGCCTAC
AAGTACCCGGCGCAGCAGGCCAAGACCGTGCTGCAAGGCGTGGTGTTCCAGGTCGGGCGGCTCGGCACCATCACGCCGGT
GGCCGAACTCAAGCCGACCAAACTCGCCGGATCGACCGTGTCGCGCTCGACGCTGCACAACTTCGACGAAATCGAACGGC
TCGGCGTGCGCATCGGCGACCACGTCATGATCGAAAAGTCGGGCGAAGTAATTCCGAAAGTGGTCAGCGTCGTGCTCGAC
GAACGCCCGGCGGAAACCGCCGAAATCGAAGCGCCGTCGGAGTGCCCGGTTTGCGGCACGAAGCTGGAGCGGCACGAAGG
CGAGGTAAGCTGGTACTGCCCCAACGAGGAGGGGTGCCCCGCGCAGAAGCGAGGCCGCATTCTGCACTTCGCCTCGCGCA
ACGCCCTCGACATCCAGAACCTCGGCGAATCGCTCGTTTCGCAGCTCGTCGAGCGCGGCTTCGTGAGCGACGCGGGCGAC
CTCTACAGCCTCACGCAGGAGCAGCTCGCGAGCCTCGACCGCATGGCCGCCAAATCGGCGCAAAACGTGCTCGACGCGCT
CGAAAAGAGCAAAAAGCAGAGCTACGCCCGCCTGCTCTTCGCCCTTGGCATTCGCCACGTCGGCGCAGCCACGGCCCGCG
AACTGGCCCACGCCTGCCCCTCCATCGACCGCCTCCGCGAGATGGGCGAGGAGGAACTCGCCGCCGTGCCCGACATCGGC
CCGGTCATCGCCGCGAGCATCCGCGACTTCTTCGCCAAACCCTGGGTGGCAGCGATGCTCGAAAAGCTCGCCGCCGCCGG
ACTGCCGATGCAGGCCGGAGAGGAAAAAGCGCTCGTCAACACCAACTTTGAAGGCCAGTCCGTCATCTTCACCGGCGGAC
TGGAACGCCACGTCCGCCAGCAAGCCGAAGAGATGGTACGCGAACGCGGCGGCAAAATCGTCAGCTCGGTGAGTAAAAAG
ACAACGCTTGTCGTTGCAGGCAAAGAGGCCGGAAGCAAGCTCGAAAAAGCGATGAAGTTGGGGGTGAGGGTGATAGACGA
AGATGAGTTCGAGCGGATGCTGTGA

Upstream 100 bases:

>100_bases
GACCAGTCTCCATCATTTTCCTTTTGTCAAGTCGGGATCGGACATCAACAAGAGCTTTCGCTCAACGAATCATTGATACC
TTTAGTTGCAGAATTTTTTT

Downstream 100 bases:

>100_bases
GGTTTTCCTGTGATTTGTCGATATTGAAATCGAACGACGGATTATTTGTAGGGGCGAACCTGTGTGTTCGCCCTCGGTGA
AATGTGAATGTCGGTGTCAG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLERLIALETEFPELVTPDSPSQRVGGGITKEFPTVIHREP
MLSLSNTYSIGEVSDFCTRVEKLVAAEGGGTPEYVAELKYDGVAISLFYRDGLLVRGSTRGDGRQGDEITANLKTIPSIP
LRLQSAGGSLFDAAVNGEVEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGHD
GEAPTHLRRLEQLKCMGFMTGAAARLCKGMDEIANFIGEWSEKRWTLPYETDGVVLKLNEVGLWDRLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTKLAGSTVSRSTLHNFDEIERLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEAPSECPVCGTKLERHEGEVSWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVSQLVERGFVSDAGD
LYSLTQEQLASLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMGEEELAAVPDIG
PVIAASIRDFFAKPWVAAMLEKLAAAGLPMQAGEEKALVNTNFEGQSVIFTGGLERHVRQQAEEMVRERGGKIVSSVSKK
TTLVVAGKEAGSKLEKAMKLGVRVIDEDEFERML

Sequences:

>Translated_674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLERLIALETEFPELVTPDSPSQRVGGGITKEFPTVIHREP
MLSLSNTYSIGEVSDFCTRVEKLVAAEGGGTPEYVAELKYDGVAISLFYRDGLLVRGSTRGDGRQGDEITANLKTIPSIP
LRLQSAGGSLFDAAVNGEVEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGHD
GEAPTHLRRLEQLKCMGFMTGAAARLCKGMDEIANFIGEWSEKRWTLPYETDGVVLKLNEVGLWDRLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTKLAGSTVSRSTLHNFDEIERLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEAPSECPVCGTKLERHEGEVSWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVSQLVERGFVSDAGD
LYSLTQEQLASLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMGEEELAAVPDIG
PVIAASIRDFFAKPWVAAMLEKLAAAGLPMQAGEEKALVNTNFEGQSVIFTGGLERHVRQQAEEMVRERGGKIVSSVSKK
TTLVVAGKEAGSKLEKAMKLGVRVIDEDEFERML
>Mature_674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLERLIALETEFPELVTPDSPSQRVGGGITKEFPTVIHREP
MLSLSNTYSIGEVSDFCTRVEKLVAAEGGGTPEYVAELKYDGVAISLFYRDGLLVRGSTRGDGRQGDEITANLKTIPSIP
LRLQSAGGSLFDAAVNGEVEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGHD
GEAPTHLRRLEQLKCMGFMTGAAARLCKGMDEIANFIGEWSEKRWTLPYETDGVVLKLNEVGLWDRLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTKLAGSTVSRSTLHNFDEIERLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEAPSECPVCGTKLERHEGEVSWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVSQLVERGFVSDAGD
LYSLTQEQLASLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMGEEELAAVPDIG
PVIAASIRDFFAKPWVAAMLEKLAAAGLPMQAGEEKALVNTNFEGQSVIFTGGLERHVRQQAEEMVRERGGKIVSSVSKK
TTLVVAGKEAGSKLEKAMKLGVRVIDEDEFERML

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=678, Percent_Identity=43.952802359882, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI87082305, Length=544, Percent_Identity=24.4485294117647, Blast_Score=132, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLP8 (B3QLI5)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998075.1
- GeneID:   6419367
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0453
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK637336
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74451; Mature: 74451

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 184-184 BINDING 297-297 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLERLIALETEFPELVTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
SQRVGGGITKEFPTVIHREPMLSLSNTYSIGEVSDFCTRVEKLVAAEGGGTPEYVAELKY
CHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHEE
DGVAISLFYRDGLLVRGSTRGDGRQGDEITANLKTIPSIPLRLQSAGGSLFDAAVNGEVE
CCEEEEEEEECCEEEECCCCCCCCCCCCEEECCCCCCCCCEEEECCCCCEEEHCCCCCEE
VRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGHD
EECEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCC
GEAPTHLRRLEQLKCMGFMTGAAARLCKGMDEIANFIGEWSEKRWTLPYETDGVVLKLNE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
VGLWDRLGATAKSPRWAIAYKYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTKLAGSTV
CCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCH
SRSTLHNFDEIERLGVRIGDHVMIEKSGEVIPKVVSVVLDERPAETAEIEAPSECPVCGT
HHHHHHCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KLERHEGEVSWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVSQLVERGFVSDAGD
HHHHCCCCEEEECCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
LYSLTQEQLASLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIDRLREMGEEELAAVPDIGPVIAASIRDFFAKPWVAAMLEKLAAAGLPMQAGEEKALVN
CHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEE
TNFEGQSVIFTGGLERHVRQQAEEMVRERGGKIVSSVSKKTTLVVAGKEAGSKLEKAMKL
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
GVRVIDEDEFERML
CCEEECCHHHHHCC
>Mature Secondary Structure
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLERLIALETEFPELVTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
SQRVGGGITKEFPTVIHREPMLSLSNTYSIGEVSDFCTRVEKLVAAEGGGTPEYVAELKY
CHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHEE
DGVAISLFYRDGLLVRGSTRGDGRQGDEITANLKTIPSIPLRLQSAGGSLFDAAVNGEVE
CCEEEEEEEECCEEEECCCCCCCCCCCCEEECCCCCCCCCEEEECCCCCEEEHCCCCCEE
VRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGHD
EECEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCC
GEAPTHLRRLEQLKCMGFMTGAAARLCKGMDEIANFIGEWSEKRWTLPYETDGVVLKLNE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
VGLWDRLGATAKSPRWAIAYKYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTKLAGSTV
CCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCH
SRSTLHNFDEIERLGVRIGDHVMIEKSGEVIPKVVSVVLDERPAETAEIEAPSECPVCGT
HHHHHHCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KLERHEGEVSWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVSQLVERGFVSDAGD
HHHHCCCCEEEECCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
LYSLTQEQLASLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIDRLREMGEEELAAVPDIGPVIAASIRDFFAKPWVAAMLEKLAAAGLPMQAGEEKALVN
CHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEE
TNFEGQSVIFTGGLERHVRQQAEEMVRERGGKIVSSVSKKTTLVVAGKEAGSKLEKAMKL
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
GVRVIDEDEFERML
CCEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA