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The map label for this gene is dagK [H]

Identifier: 193212061

GI number: 193212061

Start: 417086

End: 417994

Strand: Direct

Name: dagK [H]

Synonym: Cpar_0391

Alternate gene names: 193212061

Gene position: 417086-417994 (Clockwise)

Preceding gene: 193212060

Following gene: 193212062

Centisome position: 18.22

GC content: 58.75

Gene sequence:

>909_bases
ATGAGCGGTAACGTCACCTTTATTTTTAATCCCGCCGCCGACAAGGGACGCGCGGCTGCGAAGGCGGAAATGGTCAGGCG
TTCGCTTGCGCAGTTCGATCACTGTACGCTTGCAGAGACGCGCTTTGCGGGTCACGCCACGGAGCTCGCCCGCACTGCTG
CGAGTGACGGCGCGACGCTGATCGCGTGTGGCGGCGACGGCACGCTGAACGAGGTGGTGAACGCCGTGGTTAGTCAGCCG
GTCAGCATCGGCATCCTTCCTGTTGGTTCGGCCAACGATTTTCTGAAGAGTTTCCAGCCGTCGAAAAAAAGTGCCGAAGA
GCGCATTCGCGCCTTTTCCGCAGCTTCCAGCCGGAAGGTCGATCTCGGGCGGGTGGCGTTCAGCGAAGAGAGCCAGCGCC
TGTTCGTCAACTCCATTGGCATCGGCTTTACGGGCCGGATCGCCAGCGCGGTCAAATCGGCCAAATGGCTGCGCGGAGAG
CTCTCATACGCCTGGGCGCTGGTGAGTGTGCTCTTGGGTTATCGCCCTGTAAAAATGCATATTACGATTGATACCGCTGA
CGGCATGATCACGCTCGACGAGCCGGTATTCGCCTTTTCGGTTTCCAACGGCAAGGTCGAGGGCGGCAAGTTCCGGATTT
CGCCGGAGGCCGATCTTTATGACGGACTGCTCGACGTGTGCATTCTGAAAGCCGTTCCCAAATGGCGCGTGCCAGGCTAT
GTGCTGAAGTACCTCAAAGGCACCCAGATTCACGATGCCGAAGTCATCTACCGCAAGGCGTCCTCCATCGAGATTTTTAT
GCCGGATGAGGAGCACATGCACATCGATGGCGAGGTGATGGGTCGGGTCGGTGGCAGGATCGGTATCCACGCCGTACCGC
GCGCGGTTGAATTGCTTTATGACTCATAA

Upstream 100 bases:

>100_bases
GAGGCGGGGTTCGACCGGACGCTTCGGCGTCTCATGGAGAGCGCCACAGGCATTCTTGAAGCCATGCTTGTCGAATACGG
TTTTCCGGATGATGGAGGCC

Downstream 100 bases:

>100_bases
CCAGTTGCCCCCCTCATGCAAATCATCGTCTTTGAAGATCAGGCCGTGTTGCGGTTTTCACCGCTTGTTGACCTGAAACC
GGTTTATGATCTGGTCACCG

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSGNVTFIFNPAADKGRAAAKAEMVRRSLAQFDHCTLAETRFAGHATELARTAASDGATLIACGGDGTLNEVVNAVVSQP
VSIGILPVGSANDFLKSFQPSKKSAEERIRAFSAASSRKVDLGRVAFSEESQRLFVNSIGIGFTGRIASAVKSAKWLRGE
LSYAWALVSVLLGYRPVKMHITIDTADGMITLDEPVFAFSVSNGKVEGGKFRISPEADLYDGLLDVCILKAVPKWRVPGY
VLKYLKGTQIHDAEVIYRKASSIEIFMPDEEHMHIDGEVMGRVGGRIGIHAVPRAVELLYDS

Sequences:

>Translated_302_residues
MSGNVTFIFNPAADKGRAAAKAEMVRRSLAQFDHCTLAETRFAGHATELARTAASDGATLIACGGDGTLNEVVNAVVSQP
VSIGILPVGSANDFLKSFQPSKKSAEERIRAFSAASSRKVDLGRVAFSEESQRLFVNSIGIGFTGRIASAVKSAKWLRGE
LSYAWALVSVLLGYRPVKMHITIDTADGMITLDEPVFAFSVSNGKVEGGKFRISPEADLYDGLLDVCILKAVPKWRVPGY
VLKYLKGTQIHDAEVIYRKASSIEIFMPDEEHMHIDGEVMGRVGGRIGIHAVPRAVELLYDS
>Mature_301_residues
SGNVTFIFNPAADKGRAAAKAEMVRRSLAQFDHCTLAETRFAGHATELARTAASDGATLIACGGDGTLNEVVNAVVSQPV
SIGILPVGSANDFLKSFQPSKKSAEERIRAFSAASSRKVDLGRVAFSEESQRLFVNSIGIGFTGRIASAVKSAKWLRGEL
SYAWALVSVLLGYRPVKMHITIDTADGMITLDEPVFAFSVSNGKVEGGKFRISPEADLYDGLLDVCILKAVPKWRVPGYV
LKYLKGTQIHDAEVIYRKASSIEIFMPDEEHMHIDGEVMGRVGGRIGIHAVPRAVELLYDS

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=244, Percent_Identity=26.6393442622951, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32620; Mature: 32489

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNVTFIFNPAADKGRAAAKAEMVRRSLAQFDHCTLAETRFAGHATELARTAASDGATL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCEE
IACGGDGTLNEVVNAVVSQPVSIGILPVGSANDFLKSFQPSKKSAEERIRAFSAASSRKV
EEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
DLGRVAFSEESQRLFVNSIGIGFTGRIASAVKSAKWLRGELSYAWALVSVLLGYRPVKMH
EEHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ITIDTADGMITLDEPVFAFSVSNGKVEGGKFRISPEADLYDGLLDVCILKAVPKWRVPGY
EEEECCCCEEEECCCEEEEEECCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
VLKYLKGTQIHDAEVIYRKASSIEIFMPDEEHMHIDGEVMGRVGGRIGIHAVPRAVELLY
HHHHHCCCCCCCHHHHEEECCCEEEEECCCCCEEECHHHHHHCCCEECCHHHHHHHHHHC
DS
CC
>Mature Secondary Structure 
SGNVTFIFNPAADKGRAAAKAEMVRRSLAQFDHCTLAETRFAGHATELARTAASDGATL
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCEE
IACGGDGTLNEVVNAVVSQPVSIGILPVGSANDFLKSFQPSKKSAEERIRAFSAASSRKV
EEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
DLGRVAFSEESQRLFVNSIGIGFTGRIASAVKSAKWLRGELSYAWALVSVLLGYRPVKMH
EEHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ITIDTADGMITLDEPVFAFSVSNGKVEGGKFRISPEADLYDGLLDVCILKAVPKWRVPGY
EEEECCCCEEEECCCEEEEEECCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHH
VLKYLKGTQIHDAEVIYRKASSIEIFMPDEEHMHIDGEVMGRVGGRIGIHAVPRAVELLY
HHHHHCCCCCCCHHHHEEECCCEEEEECCCCCEEECHHHHHHCCCEECCHHHHHHHHHHC
DS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]