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The map label for this gene is dus1 [H]

Identifier: 193211956

GI number: 193211956

Start: 300880

End: 301923

Strand: Direct

Name: dus1 [H]

Synonym: Cpar_0282

Alternate gene names: 193211956

Gene position: 300880-301923 (Clockwise)

Preceding gene: 193211955

Following gene: 193211957

Centisome position: 13.14

GC content: 64.18

Gene sequence:

>1044_bases
ATGCGTATCGGACCGATCGACATCGACCGCCCCATCATCCTCGCGCCCATGGAGGATGTGACCGACCGTGCCTTCCGGCA
ACTCTGCAAGCGGCACGGGGCGGACATCGTCTATACCGAATTTGTCAGCGCGGAGGCGATCCGGCGCGGCGCGGAGAAGT
CGATACGCAAGCTCAGAATGGATGCGCACGAGCGACCCGTGGCGGTGCAGATTTTCGGCAACACGGTCGAATCGATGGTC
GAGGCGGCCTCGATTGCCGAGAGCTTCGAGCCGGACTGCCTCGACATCAACTTCGGCTGTCCGGTCAAAAAGGTGGCCGG
ACGAGGCGCGGGCGCGGCGCTGCTGAAAGAGCCGGAGAAGCTCTCCGCCATCGCCGCGGCGGTGGTTGGCGCCGTCTCGC
TGCCGGTGACTGCCAAGACGCGCATCGGCTGGGATCACGACTCGATCAACATCACCGACACCGTGCGGCGGCTCGAAGAC
GCGGGCATCCAGGCGGTCGCGGTGCACGGTCGCACGCGCAGCGATATGTACAAAGGCCGCGCAGACTGGGGGCGCATCGC
CGAAGCCAAGCGAGCCTGCTCGATTCCGGTGATCGCCAACGGCGACGTCTGGAGCGCCGATGACGCCGTGGCGATGTTCG
CTGAAACCGGCGCGGACGGCATCATGATCGGACGCGGCTCGATCGGCAACCCGTTCATTTTCGCGCAGGCTAAAAGCCTC
GTCAAGACGGGCGAGCGCCTGCCGCTTCCGTCGTATCGCGACCGGATCGACGCCGCGAGGGAGCACCTGCGCCTGTCGCT
GGAGTACAAGGGCGAAAAGCATGGCGTGCTGGAGATGCGCCGCCACTACTCGACCTACCTCAAGGGGTTGCCTGGCGTGT
CGAAAGTTCGTAACCTGTTGGTGCGCGAACCCGACCCGGCGGTGATCGTCGAACTGCTCCGGGAGTTCGAGGCAGCGTGC
GAAGCGCTCGACCGCGAGGGGCTTCTCAAAGAGGGTGGTGAATTTTTGAACGACCACTCGCCCAAACTGATTCTGAATTA
CTAA

Upstream 100 bases:

>100_bases
GCGCCTGTTGAAGGAGGACGAGACCGTGCGCAACACCATCCGCCAGCAGGTTCGCGACACGCTGACCGGAACGCCAACCG
AATAAGCCGGGCAGAACACC

Downstream 100 bases:

>100_bases
ACCTATCATTTATCTGATTTTATGAGCAGTGATGCAGGCTATCGAGTAGCCGTTCTCGGCGCAACAGGACTTGTCGGCAG
AACCATGATCAAGGTGCTCG

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRIGPIDIDRPIILAPMEDVTDRAFRQLCKRHGADIVYTEFVSAEAIRRGAEKSIRKLRMDAHERPVAVQIFGNTVESMV
EAASIAESFEPDCLDINFGCPVKKVAGRGAGAALLKEPEKLSAIAAAVVGAVSLPVTAKTRIGWDHDSINITDTVRRLED
AGIQAVAVHGRTRSDMYKGRADWGRIAEAKRACSIPVIANGDVWSADDAVAMFAETGADGIMIGRGSIGNPFIFAQAKSL
VKTGERLPLPSYRDRIDAAREHLRLSLEYKGEKHGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLREFEAAC
EALDREGLLKEGGEFLNDHSPKLILNY

Sequences:

>Translated_347_residues
MRIGPIDIDRPIILAPMEDVTDRAFRQLCKRHGADIVYTEFVSAEAIRRGAEKSIRKLRMDAHERPVAVQIFGNTVESMV
EAASIAESFEPDCLDINFGCPVKKVAGRGAGAALLKEPEKLSAIAAAVVGAVSLPVTAKTRIGWDHDSINITDTVRRLED
AGIQAVAVHGRTRSDMYKGRADWGRIAEAKRACSIPVIANGDVWSADDAVAMFAETGADGIMIGRGSIGNPFIFAQAKSL
VKTGERLPLPSYRDRIDAAREHLRLSLEYKGEKHGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLREFEAAC
EALDREGLLKEGGEFLNDHSPKLILNY
>Mature_347_residues
MRIGPIDIDRPIILAPMEDVTDRAFRQLCKRHGADIVYTEFVSAEAIRRGAEKSIRKLRMDAHERPVAVQIFGNTVESMV
EAASIAESFEPDCLDINFGCPVKKVAGRGAGAALLKEPEKLSAIAAAVVGAVSLPVTAKTRIGWDHDSINITDTVRRLED
AGIQAVAVHGRTRSDMYKGRADWGRIAEAKRACSIPVIANGDVWSADDAVAMFAETGADGIMIGRGSIGNPFIFAQAKSL
VKTGERLPLPSYRDRIDAAREHLRLSLEYKGEKHGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLREFEAAC
EALDREGLLKEGGEFLNDHSPKLILNY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=289, Percent_Identity=31.4878892733564, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=31.4878892733564, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=35.2173913043478, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI31742496, Length=253, Percent_Identity=31.2252964426877, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=30.6122448979592, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1789660, Length=292, Percent_Identity=40.0684931506849, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788462, Length=252, Percent_Identity=35.7142857142857, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693211, Length=237, Percent_Identity=30.379746835443, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=33.7552742616034, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=28.125, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=249, Percent_Identity=30.1204819277108, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=281, Percent_Identity=29.8932384341637, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=235, Percent_Identity=32.7659574468085, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=308, Percent_Identity=30.1948051948052, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=317, Percent_Identity=24.9211356466877, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24580595, Length=228, Percent_Identity=35.0877192982456, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920448, Length=228, Percent_Identity=35.0877192982456, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24585320, Length=284, Percent_Identity=29.5774647887324, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921524, Length=251, Percent_Identity=27.4900398406374, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45549423, Length=292, Percent_Identity=29.1095890410959, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38065; Mature: 38065

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGPIDIDRPIILAPMEDVTDRAFRQLCKRHGADIVYTEFVSAEAIRRGAEKSIRKLRM
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
DAHERPVAVQIFGNTVESMVEAASIAESFEPDCLDINFGCPVKKVAGRGAGAALLKEPEK
CCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHHCCHHH
LSAIAAAVVGAVSLPVTAKTRIGWDHDSINITDTVRRLEDAGIQAVAVHGRTRSDMYKGR
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHCCC
ADWGRIAEAKRACSIPVIANGDVWSADDAVAMFAETGADGIMIGRGSIGNPFIFAQAKSL
CHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
VKTGERLPLPSYRDRIDAAREHLRLSLEYKGEKHGVLEMRRHYSTYLKGLPGVSKVRNLL
HHCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VREPDPAVIVELLREFEAACEALDREGLLKEGGEFLNDHSPKLILNY
HCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCCCEEEEEC
>Mature Secondary Structure
MRIGPIDIDRPIILAPMEDVTDRAFRQLCKRHGADIVYTEFVSAEAIRRGAEKSIRKLRM
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
DAHERPVAVQIFGNTVESMVEAASIAESFEPDCLDINFGCPVKKVAGRGAGAALLKEPEK
CCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHHCCHHH
LSAIAAAVVGAVSLPVTAKTRIGWDHDSINITDTVRRLEDAGIQAVAVHGRTRSDMYKGR
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHCCC
ADWGRIAEAKRACSIPVIANGDVWSADDAVAMFAETGADGIMIGRGSIGNPFIFAQAKSL
CHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
VKTGERLPLPSYRDRIDAAREHLRLSLEYKGEKHGVLEMRRHYSTYLKGLPGVSKVRNLL
HHCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VREPDPAVIVELLREFEAACEALDREGLLKEGGEFLNDHSPKLILNY
HCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]