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The map label for this gene is galE [C]

Identifier: 193211939

GI number: 193211939

Start: 283567

End: 284568

Strand: Direct

Name: galE [C]

Synonym: Cpar_0265

Alternate gene names: 193211939

Gene position: 283567-284568 (Clockwise)

Preceding gene: 193211929

Following gene: 193211943

Centisome position: 12.39

GC content: 60.78

Gene sequence:

>1002_bases
ATGGATGAAATGATTCTGGTTACAGGCTCGACCGGGTTTATCGGATCGAGGATGGTTGACAGGCTTGTCGGCGAAGGGTG
CCGTGTCCGGGTGCTGCTTCGGCCCGAAAGCGCATCCTTTTCAGCCGCCTCTGGCCGGAACGGCGTCGAGACGGTTCGAG
CCGCTTACGATGATGCCGAAGCACTCGGCAGGGCGGTGTCGGGCGTCGATTCGATCATCCATCTGGCCGGGCTGACCAAG
GCGGCTGATGAAGCGGGCTTTATCTCGGGCAACGTGATGCCGGTGGAAAACCTGCTCGGTGCGGTGCAACGGCACAATCC
GGGGCTCAGGCGTTTTCTGCTTGTCTCCTCGCTTGCTGCTGCGGGGCCTGCCCAGTCGCCATCGCCCGGCGTCAGGGAGT
CTGATAGTCCTGCTCCTGTCAGCGCGTATGGACGAAGCAAGCTGCTCGGCGAAGAAGCTGCTCTGCGCCACGCCGGAGCG
GTGCCGCTTACCATCGTTCGTCCTCCGGCGGTTTACGGGCCGGGAGATCGCGACATTCTGGAAGTATTTGCCATGATGAA
GAAGGGGTATCTGCTCTCGGCGGGTTCCGGAGTGCGACAGCGTTTCAGCATGATTCATGTCGATGATCTGGTTGAGGGAA
TGTTGCGTGCGCTCCGTTCAGAGGCCGGCGCTGGCAGCACCTATTTCATCACCTCGCCGCGTGGATACGCCTGGAGTGAA
GTGATCGAGGCCGCACGGCCGGTGCTCGGTTTCGGGAAACTCGTGCGCATCAATCTGCCCAAGCCGCTGGTGTTCGGCCT
CGGCTCGGTGCTTGAAGCCTTCGCGAAGTTGAGTGGTCGTCCGGCACTGATTAACCGCGACAAAGCCGCCGAGCTGGTGC
AGGATTACTGGGTTTGCAGTTCCGAAAAAGCCGAAAAGGAGTTGGGGTTTGTTGCGCCCACGTCGTTGGCCGAGGGCGTG
GCTGAAACGCTACAGTGGTATCAGCGAAAAGGGTGGCTATGA

Upstream 100 bases:

>100_bases
TAAAGCGCCCTATGTTTTTTCTTTGGCTAAAGAACCCGTCCGTTTGCTGGCCGCATTGGGGCGGGGAGCTGGTAACGATT
GTAAACAGCCTTTGGAAAGT

Downstream 100 bases:

>100_bases
AGGGTGAGAGCTGGCATGGTTCACCCCGGCTTGAAAGCCGGAGCAATTGAAGAAAATAGAGGAGGTTGAATGTCCGTCGG
GCTAAAGCCCTCCTGAATGC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDEMILVTGSTGFIGSRMVDRLVGEGCRVRVLLRPESASFSAASGRNGVETVRAAYDDAEALGRAVSGVDSIIHLAGLTK
AADEAGFISGNVMPVENLLGAVQRHNPGLRRFLLVSSLAAAGPAQSPSPGVRESDSPAPVSAYGRSKLLGEEAALRHAGA
VPLTIVRPPAVYGPGDRDILEVFAMMKKGYLLSAGSGVRQRFSMIHVDDLVEGMLRALRSEAGAGSTYFITSPRGYAWSE
VIEAARPVLGFGKLVRINLPKPLVFGLGSVLEAFAKLSGRPALINRDKAAELVQDYWVCSSEKAEKELGFVAPTSLAEGV
AETLQWYQRKGWL

Sequences:

>Translated_333_residues
MDEMILVTGSTGFIGSRMVDRLVGEGCRVRVLLRPESASFSAASGRNGVETVRAAYDDAEALGRAVSGVDSIIHLAGLTK
AADEAGFISGNVMPVENLLGAVQRHNPGLRRFLLVSSLAAAGPAQSPSPGVRESDSPAPVSAYGRSKLLGEEAALRHAGA
VPLTIVRPPAVYGPGDRDILEVFAMMKKGYLLSAGSGVRQRFSMIHVDDLVEGMLRALRSEAGAGSTYFITSPRGYAWSE
VIEAARPVLGFGKLVRINLPKPLVFGLGSVLEAFAKLSGRPALINRDKAAELVQDYWVCSSEKAEKELGFVAPTSLAEGV
AETLQWYQRKGWL
>Mature_333_residues
MDEMILVTGSTGFIGSRMVDRLVGEGCRVRVLLRPESASFSAASGRNGVETVRAAYDDAEALGRAVSGVDSIIHLAGLTK
AADEAGFISGNVMPVENLLGAVQRHNPGLRRFLLVSSLAAAGPAQSPSPGVRESDSPAPVSAYGRSKLLGEEAALRHAGA
VPLTIVRPPAVYGPGDRDILEVFAMMKKGYLLSAGSGVRQRFSMIHVDDLVEGMLRALRSEAGAGSTYFITSPRGYAWSE
VIEAARPVLGFGKLVRINLPKPLVFGLGSVLEAFAKLSGRPALINRDKAAELVQDYWVCSSEKAEKELGFVAPTSLAEGV
AETLQWYQRKGWL

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35409; Mature: 35409

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEMILVTGSTGFIGSRMVDRLVGEGCRVRVLLRPESASFSAASGRNGVETVRAAYDDAE
CCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ALGRAVSGVDSIIHLAGLTKAADEAGFISGNVMPVENLLGAVQRHNPGLRRFLLVSSLAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AGPAQSPSPGVRESDSPAPVSAYGRSKLLGEEAALRHAGAVPLTIVRPPAVYGPGDRDIL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHH
EVFAMMKKGYLLSAGSGVRQRFSMIHVDDLVEGMLRALRSEAGAGSTYFITSPRGYAWSE
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
VIEAARPVLGFGKLVRINLPKPLVFGLGSVLEAFAKLSGRPALINRDKAAELVQDYWVCS
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
SEKAEKELGFVAPTSLAEGVAETLQWYQRKGWL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDEMILVTGSTGFIGSRMVDRLVGEGCRVRVLLRPESASFSAASGRNGVETVRAAYDDAE
CCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ALGRAVSGVDSIIHLAGLTKAADEAGFISGNVMPVENLLGAVQRHNPGLRRFLLVSSLAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AGPAQSPSPGVRESDSPAPVSAYGRSKLLGEEAALRHAGAVPLTIVRPPAVYGPGDRDIL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHH
EVFAMMKKGYLLSAGSGVRQRFSMIHVDDLVEGMLRALRSEAGAGSTYFITSPRGYAWSE
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
VIEAARPVLGFGKLVRINLPKPLVFGLGSVLEAFAKLSGRPALINRDKAAELVQDYWVCS
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
SEKAEKELGFVAPTSLAEGVAETLQWYQRKGWL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]