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The map label for this gene is miaB

Identifier: 193211917

GI number: 193211917

Start: 253047

End: 254378

Strand: Direct

Name: miaB

Synonym: Cpar_0243

Alternate gene names: 193211917

Gene position: 253047-254378 (Clockwise)

Preceding gene: 193211916

Following gene: 193211918

Centisome position: 11.05

GC content: 62.61

Gene sequence:

>1332_bases
ATGTCACCAACCTTTTTCATCCACACTTTCGGTTGCCAGATGAACATGGCTGACACGGAGATCGTCACGGCGATTCTCGT
CGAGGGCGGTTTTGCGCCGGCTGAGGATGAAGGCACGGCGGACATGGTGCTGCTCAACACCTGCGCCGTGCGGGCCAACG
CGGTGGATCGCGCAGGGAACGTGCTCAGCCATCTCAAGGGGATGAAGCGGCGGCGGAAGGGTCTCGTCGTCGGCCTTCTC
GGCTGCGTGCCGCAGTACGAGCGCGAGCAGCTTTTCGGTGACTTTCCGTTCGTGGATTTCATTGTCGGCCCCGACAACTA
CCGCGACCTGTGCGGCATCGTCCGCAGCGTGCGTGAGGGGGAGCAGCGCCGAGCGTTCATCGACTACGACCAGCAGGAGA
CCTACGCGGGCATCGATCCGATTCGCGCCAACCGCGTCAGCACCTTTCTGCCGGTCATGCGCGGCTGCAACAATCACTGC
GCCTTCTGTGTGGTGCCGGTGACGCGCGGGCGCGAGCGCAGCGTAGCGTTCGACCGGGTCATCGCCGAGGTTGCTGCTCT
GGAACAAGCGGGCTACCGCGAAGTGACGCTGCTCGGCCAGAACGTCAACTCGTGGTGCGATGCCGACAAGTCGCTCGACT
TTGCCGGGCTGCTCGAAGGGGTGAGCCACGCTGCGCCGTCGATGCGCATCCGCTTCACCACCTCGCACCCCAAGGACATC
TCCGAATCGCTCGTGCGGGTGATCGGCGAGCGGCCGAACCTCTGCAAGCACATCCATCTTCCCGTGCAGTCCGGCTCGTC
GCGGATGCTCGATCTGATGAAGCGCGGTCACACCCGCGAGGAGTATCTCGACAAAATCGCCATGATCCGAGAGCTTGTGC
CTGGCGTGGCCATCACCACCGACCTGATCGCCGGATTCTGCACCGAGACAGAGGTGGATCACCGCGAGACTCTTTCGCTG
ATGGAGGCGGTCGGCTACGATACGGCGTTCATGTTCTACTACTCGGTGCGTCCGGGCACCTGGGCGGCGCGGAATCTCCC
CGACGATGTGCCTGAAGCGGTCAAGAAGGCGCGGCTTCAGGAGATCATTGATTTGCAGACCGCGATGTCTCGTGAGCTTT
ACCAGCGGGAGATCGGCAAGGTGGTCGAGGTGCTTGCCGAAGCCGAAAGCAAGCGCTCCGCATCGCAACTCATGGGGCGC
ACCAGCGAAAACCGGGCCGTGGTGTTCAGCCGGGAGCATTTCATGCCGGGTGATCTGGTGCCAGTCAGAATTACTGCGGC
TACATCGGCCACGCTCTCCGGTGAGGCTCTTGTCCAACCTTCCAATCCCTAA

Upstream 100 bases:

>100_bases
CGGAGAGCGTGCTTCGTGATGCACCCGGTTTTCCGGGCAATTCACGAGGCGCGCCTCTTCCCGTTATAGTCATCTACCCT
TTGATCAACGAGTGAAGAAC

Downstream 100 bases:

>100_bases
AATCGTCAAAACAGGGTAGTTTCCAGCATCGGTTGATGACCTTGAGGCTGCATCGTATCGGGCTGCATCCATAATTGTTC
CCGATATGATGTTTTTATTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSPTFFIHTFGCQMNMADTEIVTAILVEGGFAPAEDEGTADMVLLNTCAVRANAVDRAGNVLSHLKGMKRRRKGLVVGLL
GCVPQYEREQLFGDFPFVDFIVGPDNYRDLCGIVRSVREGEQRRAFIDYDQQETYAGIDPIRANRVSTFLPVMRGCNNHC
AFCVVPVTRGRERSVAFDRVIAEVAALEQAGYREVTLLGQNVNSWCDADKSLDFAGLLEGVSHAAPSMRIRFTTSHPKDI
SESLVRVIGERPNLCKHIHLPVQSGSSRMLDLMKRGHTREEYLDKIAMIRELVPGVAITTDLIAGFCTETEVDHRETLSL
MEAVGYDTAFMFYYSVRPGTWAARNLPDDVPEAVKKARLQEIIDLQTAMSRELYQREIGKVVEVLAEAESKRSASQLMGR
TSENRAVVFSREHFMPGDLVPVRITAATSATLSGEALVQPSNP

Sequences:

>Translated_443_residues
MSPTFFIHTFGCQMNMADTEIVTAILVEGGFAPAEDEGTADMVLLNTCAVRANAVDRAGNVLSHLKGMKRRRKGLVVGLL
GCVPQYEREQLFGDFPFVDFIVGPDNYRDLCGIVRSVREGEQRRAFIDYDQQETYAGIDPIRANRVSTFLPVMRGCNNHC
AFCVVPVTRGRERSVAFDRVIAEVAALEQAGYREVTLLGQNVNSWCDADKSLDFAGLLEGVSHAAPSMRIRFTTSHPKDI
SESLVRVIGERPNLCKHIHLPVQSGSSRMLDLMKRGHTREEYLDKIAMIRELVPGVAITTDLIAGFCTETEVDHRETLSL
MEAVGYDTAFMFYYSVRPGTWAARNLPDDVPEAVKKARLQEIIDLQTAMSRELYQREIGKVVEVLAEAESKRSASQLMGR
TSENRAVVFSREHFMPGDLVPVRITAATSATLSGEALVQPSNP
>Mature_442_residues
SPTFFIHTFGCQMNMADTEIVTAILVEGGFAPAEDEGTADMVLLNTCAVRANAVDRAGNVLSHLKGMKRRRKGLVVGLLG
CVPQYEREQLFGDFPFVDFIVGPDNYRDLCGIVRSVREGEQRRAFIDYDQQETYAGIDPIRANRVSTFLPVMRGCNNHCA
FCVVPVTRGRERSVAFDRVIAEVAALEQAGYREVTLLGQNVNSWCDADKSLDFAGLLEGVSHAAPSMRIRFTTSHPKDIS
ESLVRVIGERPNLCKHIHLPVQSGSSRMLDLMKRGHTREEYLDKIAMIRELVPGVAITTDLIAGFCTETEVDHRETLSLM
EAVGYDTAFMFYYSVRPGTWAARNLPDDVPEAVKKARLQEIIDLQTAMSRELYQREIGKVVEVLAEAESKRSASQLMGRT
SENRAVVFSREHFMPGDLVPVRITAATSATLSGEALVQPSNP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=41.6317991631799, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=41.9831223628692, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI93277076, Length=441, Percent_Identity=27.437641723356, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=37.9775280898876, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=25.3863134657837, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=41.541755888651, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI71996771, Length=348, Percent_Identity=26.7241379310345, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=40, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI19922432, Length=446, Percent_Identity=28.0269058295964, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CHLP8 (B3QR49)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001997870.1
- GeneID:   6419156
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0243
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14340
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49152; Mature: 49021

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTFFIHTFGCQMNMADTEIVTAILVEGGFAPAEDEGTADMVLLNTCAVRANAVDRAGN
CCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
VLSHLKGMKRRRKGLVVGLLGCVPQYEREQLFGDFPFVDFIVGPDNYRDLCGIVRSVREG
HHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EQRRAFIDYDQQETYAGIDPIRANRVSTFLPVMRGCNNHCAFCVVPVTRGRERSVAFDRV
HHCCEECCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
IAEVAALEQAGYREVTLLGQNVNSWCDADKSLDFAGLLEGVSHAAPSMRIRFTTSHPKDI
HHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHH
SESLVRVIGERPNLCKHIHLPVQSGSSRMLDLMKRGHTREEYLDKIAMIRELVPGVAITT
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHH
DLIAGFCTETEVDHRETLSLMEAVGYDTAFMFYYSVRPGTWAARNLPDDVPEAVKKARLQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
EIIDLQTAMSRELYQREIGKVVEVLAEAESKRSASQLMGRTSENRAVVFSREHFMPGDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
PVRITAATSATLSGEALVQPSNP
EEEEEEECCCCCCCCEEECCCCC
>Mature Secondary Structure 
SPTFFIHTFGCQMNMADTEIVTAILVEGGFAPAEDEGTADMVLLNTCAVRANAVDRAGN
CCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
VLSHLKGMKRRRKGLVVGLLGCVPQYEREQLFGDFPFVDFIVGPDNYRDLCGIVRSVREG
HHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EQRRAFIDYDQQETYAGIDPIRANRVSTFLPVMRGCNNHCAFCVVPVTRGRERSVAFDRV
HHCCEECCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
IAEVAALEQAGYREVTLLGQNVNSWCDADKSLDFAGLLEGVSHAAPSMRIRFTTSHPKDI
HHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHH
SESLVRVIGERPNLCKHIHLPVQSGSSRMLDLMKRGHTREEYLDKIAMIRELVPGVAITT
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHH
DLIAGFCTETEVDHRETLSLMEAVGYDTAFMFYYSVRPGTWAARNLPDDVPEAVKKARLQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
EIIDLQTAMSRELYQREIGKVVEVLAEAESKRSASQLMGRTSENRAVVFSREHFMPGDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
PVRITAATSATLSGEALVQPSNP
EEEEEEECCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA