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The map label for this gene is kefA [H]

Identifier: 193211897

GI number: 193211897

Start: 229614

End: 230519

Strand: Direct

Name: kefA [H]

Synonym: Cpar_0223

Alternate gene names: 193211897

Gene position: 229614-230519 (Clockwise)

Preceding gene: 193211896

Following gene: 193211898

Centisome position: 10.03

GC content: 51.88

Gene sequence:

>906_bases
ATGGAATCGACAAATTTGCATGAACTGACCTCTGTTTTTGACTGGCTTGTTCGTCCGTTGTTTGCAATCGGCAAATCCGA
GATTTCGATTTTCAAGATTGCGAGCATTATCCTTTCTGTTGCCGTCATCGTTGCGTCAGCGAAGTTTCTGAAGCGTCTGC
TGGTCGGAAAACTTCTGGTTTCTTCTGTGCATGATAAAGGGACCCGCTCAGCCCTCGGCACAATCGTTCAGTACCTGATC
GTCTTTTTCGGTTTTCTGATCGTGCTGCAATCGGCGGGTATTGACCTGAGTACGCTGACCGTGCTGTCGGGTACCATCGG
TCTTGGTATCGGTTTCGGTTTGCAGAATATCGCCGATAACTTTTTCAGTGGCCTGATCATTCTGCTCGAACGCCCGGTCA
AGGTTGGTGACCGGATTCAGGTCGGCGACATCAATGGCGACGTGGTTCGGATCGCCATTCGTTCCACAACCGTGCTGACC
AACGACAATATCAACATCATCATTCCCAATTCGGAGTTTGTCTCAAAGCAGGTAATCAACTGGAGCCACAACGACCGCAA
TCTTCGCGTGTCGGTTCCGGTCGGGGTCTCCTATGGCTCCAATCCCGAACAGGTAAAAACGGTGCTGCTCAGCGTCGCAG
ACAATCATCCCGATATTCTCGCCAAACCAGCGCCTGCCGTGCTTTTTACCGGTTTTGGCAACAGTTCACTCGATTTTGAG
CTGCTGGTGTGGACCCAGACGCGCATCCAGACACCGAGGTTTCTGCGCAGTGAGCTGAACTACCGGATCTTTGCTGCGTT
TCGCAAGAGCGGGATCGAAATTCCTTTTCCGCAAACCGATCTTCACATCAGGAGCACAGACATTCCGCTTTGGGAGCCTC
GCGCTCCGAAGAGCGCTTCCAGTTGA

Upstream 100 bases:

>100_bases
GTATTGGGGAAGCGGCGAGAGCTTGGTAAGGCATAGTAGGCGGGTTGGACGGCATCGCCATCTGCCAGTTGTTTGGTAAT
ATTTTGGAGCTAAATGCCTT

Downstream 100 bases:

>100_bases
GCTGCATTTTGCTCGCGGGTCGCGCTTGATTTGGTTTTATCGGAATGTATTGCTAAATAACAAGACGAGATTTTTTATTC
ACGGGCGGGCATCACAGACG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MESTNLHELTSVFDWLVRPLFAIGKSEISIFKIASIILSVAVIVASAKFLKRLLVGKLLVSSVHDKGTRSALGTIVQYLI
VFFGFLIVLQSAGIDLSTLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGDINGDVVRIAIRSTTVLT
NDNINIIIPNSEFVSKQVINWSHNDRNLRVSVPVGVSYGSNPEQVKTVLLSVADNHPDILAKPAPAVLFTGFGNSSLDFE
LLVWTQTRIQTPRFLRSELNYRIFAAFRKSGIEIPFPQTDLHIRSTDIPLWEPRAPKSASS

Sequences:

>Translated_301_residues
MESTNLHELTSVFDWLVRPLFAIGKSEISIFKIASIILSVAVIVASAKFLKRLLVGKLLVSSVHDKGTRSALGTIVQYLI
VFFGFLIVLQSAGIDLSTLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGDINGDVVRIAIRSTTVLT
NDNINIIIPNSEFVSKQVINWSHNDRNLRVSVPVGVSYGSNPEQVKTVLLSVADNHPDILAKPAPAVLFTGFGNSSLDFE
LLVWTQTRIQTPRFLRSELNYRIFAAFRKSGIEIPFPQTDLHIRSTDIPLWEPRAPKSASS
>Mature_301_residues
MESTNLHELTSVFDWLVRPLFAIGKSEISIFKIASIILSVAVIVASAKFLKRLLVGKLLVSSVHDKGTRSALGTIVQYLI
VFFGFLIVLQSAGIDLSTLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGDINGDVVRIAIRSTTVLT
NDNINIIIPNSEFVSKQVINWSHNDRNLRVSVPVGVSYGSNPEQVKTVLLSVADNHPDILAKPAPAVLFTGFGNSSLDFE
LLVWTQTRIQTPRFLRSELNYRIFAAFRKSGIEIPFPQTDLHIRSTDIPLWEPRAPKSASS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=256, Percent_Identity=37.890625, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI2367355, Length=268, Percent_Identity=32.089552238806, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789291, Length=231, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787591, Length=222, Percent_Identity=29.2792792792793, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 33020; Mature: 33020

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTNLHELTSVFDWLVRPLFAIGKSEISIFKIASIILSVAVIVASAKFLKRLLVGKLLV
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVHDKGTRSALGTIVQYLIVFFGFLIVLQSAGIDLSTLTVLSGTIGLGIGFGLQNIADN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FFSGLIILLERPVKVGDRIQVGDINGDVVRIAIRSTTVLTNDNINIIIPNSEFVSKQVIN
HHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHHC
WSHNDRNLRVSVPVGVSYGSNPEQVKTVLLSVADNHPDILAKPAPAVLFTGFGNSSLDFE
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCEE
LLVWTQTRIQTPRFLRSELNYRIFAAFRKSGIEIPFPQTDLHIRSTDIPLWEPRAPKSAS
EEEEEHHHHCCHHHHHHHCCCCEEEEHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MESTNLHELTSVFDWLVRPLFAIGKSEISIFKIASIILSVAVIVASAKFLKRLLVGKLLV
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVHDKGTRSALGTIVQYLIVFFGFLIVLQSAGIDLSTLTVLSGTIGLGIGFGLQNIADN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FFSGLIILLERPVKVGDRIQVGDINGDVVRIAIRSTTVLTNDNINIIIPNSEFVSKQVIN
HHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHHC
WSHNDRNLRVSVPVGVSYGSNPEQVKTVLLSVADNHPDILAKPAPAVLFTGFGNSSLDFE
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCEE
LLVWTQTRIQTPRFLRSELNYRIFAAFRKSGIEIPFPQTDLHIRSTDIPLWEPRAPKSAS
EEEEEHHHHCCHHHHHHHCCCCEEEEHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]