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The map label for this gene is leuA [H]

Identifier: 193211812

GI number: 193211812

Start: 154218

End: 155906

Strand: Direct

Name: leuA [H]

Synonym: Cpar_0137

Alternate gene names: 193211812

Gene position: 154218-155906 (Clockwise)

Preceding gene: 193211811

Following gene: 193211813

Centisome position: 6.74

GC content: 59.33

Gene sequence:

>1689_bases
ATGAGTACGATGAATTACCGGAAATATGCATCCTATACGCCGGTCGGCCTGAAAGACCGTACCTGGCCCGACAAAATCAT
TACCAAAGCGCCGATCTGGTGCAGCGTGGATCTGCGTGACGGCAACCAGGCACTGCCGATTCCCATGAGCGTCGAAGAGA
AGGTCGAATTTTTCAAGCTGCTGGTCTCTGTCGGCTTCAAGGAGATCGAAGTCGGCTTTCCGTCAGCTTCGGCTACGGAG
TTCGCGTTCGTGCGCCGGTTGATCGAGGAAAATATCATCCCGGATGATGTGAGCATTCAGGTGCTGACCCAGTCGCGCGA
ACATCTGATCCGCCGCACCTTCGAGGCAATCAAGGGGGCGAAAAACGCTATCGTGCACCTCTACAACTCGACCTCACGCC
AGCAGCGTGACGTGGTGTTCCGCATGAGCCGCGAGGAGATTATCTCCATCGCTGTGGCCGGCACGCGCCTGGTTCGCGAG
CTGAAAGAGGAAAGTGGCAATCCGGGCATCCGCTTCGAGTACAGCCCCGAAAGCTTCACCGGCACCGAGCTGGACTACGC
GCTGGACGTGTGCCACGCGGTAATGGACGAGTGGGGCGCATCGGCGGAGAACAAGGTGATCCTGAACCTGCCCTCCACGG
TCGAGCTTTCGACTCCGAACGTCTACGCCGACCGCATCGAGTGGTTTTGCCGCAACATCAAGGATCGCGATGCGGTACTC
TTCAGCGTGCACGCTCACAACGACCGCGGCACGGCGGTGGCAACCTCCGAGCTGGCCGTGATGGCGGGCGCGGATCGCAT
CGAGGGCGCGCTGTTCGGCAATGGTGAGCGGTGCGGCAACATGGACATCATCATCATGGCGCTGAACCTGATGACGCAAG
GTGTCGATCCGGAGCTCGATTTCTCCAACCTGCCGCATATCAAGCAGGTCTACTCCCGCTGCACCCGCATGACTGTGCAT
CCGCGCCACCCTTACTCGGGCGATCTGGTCTATACGGCCTTCTCTGGCTCGCACCAGGATGCCATCAGCAAGGGCATGAA
AATGCACAAGCGCGCTCCCGAAGGCATCTGGGATGTGCCCTATCTGCCGATCGACCCGCAGGATGTTGGCTGCAACTACG
AGGCGATCGTGAGAATCAACAGTCAGTCGGGCAAGGGTGGCATCGCTTATCTTCTGGAAAAACAGTACGGCATCCAGATT
CCGAAGTGGATGCAGCCCGATTTCGGTACGGCCGTGCAGGAGGTGACCGACCGCACCGGCGAGGAGCTGAGCGCCGAACA
GATTCACGAGCTGTTCCAGAAGGAGTACATCGGCGCAACCGAGCCCTATCTGATGAAGAAGTGCACTATCAGCTGGAGCG
ACGAAGATCCGGATCGCAACGACGAGGTCGCCACTATCGTCAGCGCCTCGATGGTCGGCCCCAAGGGCGAGTTCACCTTC
AAGGCGAAGGGCAACGGGCCGCTGGATGCGTTTGTGCGCGGCATGGTGAGCCACACCGGCATCGATTTCCATGTCGATGA
ATACACGGAGCACGCCATAGGCCACAGCTCCGACGCGATGGCTATCGCCTACATCCGCCTCTCGTTCGATGACGTGGCGC
TGGTGTGCGGATCGGGCATCGACTCGAACATCAGCCTCGCCTCCATCAAAGCGATCGTCAGTGCGCTGAACCGCTACAAA
AAGGCTTGA

Upstream 100 bases:

>100_bases
GCATGAAATTCAAGTTTAAAGCCGCAATCCTCTCAGGGTAAGCGGCTTTTTTACTTTACAGGACTTTACCTTTCAATTGA
GAATAAATGGAGACTCAGTC

Downstream 100 bases:

>100_bases
TCGTTTTTCTCGTCAGGCTTCCCGATGATGGGGAAGCCTGACCCCCTCCAACGTTTTGGATTCTCTGCCGTTACCCTATC
TTTACAAAAGAGACACTGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSTMNYRKYASYTPVGLKDRTWPDKIITKAPIWCSVDLRDGNQALPIPMSVEEKVEFFKLLVSVGFKEIEVGFPSASATE
FAFVRRLIEENIIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDVVFRMSREEIISIAVAGTRLVRE
LKEESGNPGIRFEYSPESFTGTELDYALDVCHAVMDEWGASAENKVILNLPSTVELSTPNVYADRIEWFCRNIKDRDAVL
FSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVH
PRHPYSGDLVYTAFSGSHQDAISKGMKMHKRAPEGIWDVPYLPIDPQDVGCNYEAIVRINSQSGKGGIAYLLEKQYGIQI
PKWMQPDFGTAVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPKGEFTF
KAKGNGPLDAFVRGMVSHTGIDFHVDEYTEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYK
KA

Sequences:

>Translated_562_residues
MSTMNYRKYASYTPVGLKDRTWPDKIITKAPIWCSVDLRDGNQALPIPMSVEEKVEFFKLLVSVGFKEIEVGFPSASATE
FAFVRRLIEENIIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDVVFRMSREEIISIAVAGTRLVRE
LKEESGNPGIRFEYSPESFTGTELDYALDVCHAVMDEWGASAENKVILNLPSTVELSTPNVYADRIEWFCRNIKDRDAVL
FSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVH
PRHPYSGDLVYTAFSGSHQDAISKGMKMHKRAPEGIWDVPYLPIDPQDVGCNYEAIVRINSQSGKGGIAYLLEKQYGIQI
PKWMQPDFGTAVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPKGEFTF
KAKGNGPLDAFVRGMVSHTGIDFHVDEYTEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYK
KA
>Mature_561_residues
STMNYRKYASYTPVGLKDRTWPDKIITKAPIWCSVDLRDGNQALPIPMSVEEKVEFFKLLVSVGFKEIEVGFPSASATEF
AFVRRLIEENIIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDVVFRMSREEIISIAVAGTRLVREL
KEESGNPGIRFEYSPESFTGTELDYALDVCHAVMDEWGASAENKVILNLPSTVELSTPNVYADRIEWFCRNIKDRDAVLF
SVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVHP
RHPYSGDLVYTAFSGSHQDAISKGMKMHKRAPEGIWDVPYLPIDPQDVGCNYEAIVRINSQSGKGGIAYLLEKQYGIQIP
KWMQPDFGTAVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPKGEFTFK
AKGNGPLDAFVRGMVSHTGIDFHVDEYTEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYKK
A

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=544, Percent_Identity=25, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=46.524064171123, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324682, Length=564, Percent_Identity=45.3900709219858, Blast_Score=511, Evalue=1e-145,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62631; Mature: 62500

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMNYRKYASYTPVGLKDRTWPDKIITKAPIWCSVDLRDGNQALPIPMSVEEKVEFFKL
CCCCCCCHHCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRRLIEENIIPDDVSIQVLTQSREHLIRRTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
KNAIVHLYNSTSRQQRDVVFRMSREEIISIAVAGTRLVRELKEESGNPGIRFEYSPESFT
CCEEEEEECCCCCHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GTELDYALDVCHAVMDEWGASAENKVILNLPSTVELSTPNVYADRIEWFCRNIKDRDAVL
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCEEE
FSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELD
EEEEECCCCCCEEEECCEEEEECCHHHCCEEECCCCCCCCCEEEEEEHHHHHCCCCCCCC
FSNLPHIKQVYSRCTRMTVHPRHPYSGDLVYTAFSGSHQDAISKGMKMHKRAPEGIWDVP
CCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
YLPIDPQDVGCNYEAIVRINSQSGKGGIAYLLEKQYGIQIPKWMQPDFGTAVQEVTDRTG
CCCCCCHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
EELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPKGEFTF
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHEEEHHHCCCCCCEEE
KAKGNGPLDAFVRGMVSHTGIDFHVDEYTEHAIGHSSDAMAIAYIRLSFDDVALVCGSGI
EECCCCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCC
DSNISLASIKAIVSALNRYKKA
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STMNYRKYASYTPVGLKDRTWPDKIITKAPIWCSVDLRDGNQALPIPMSVEEKVEFFKL
CCCCCCHHCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRRLIEENIIPDDVSIQVLTQSREHLIRRTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
KNAIVHLYNSTSRQQRDVVFRMSREEIISIAVAGTRLVRELKEESGNPGIRFEYSPESFT
CCEEEEEECCCCCHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GTELDYALDVCHAVMDEWGASAENKVILNLPSTVELSTPNVYADRIEWFCRNIKDRDAVL
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCEEE
FSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELD
EEEEECCCCCCEEEECCEEEEECCHHHCCEEECCCCCCCCCEEEEEEHHHHHCCCCCCCC
FSNLPHIKQVYSRCTRMTVHPRHPYSGDLVYTAFSGSHQDAISKGMKMHKRAPEGIWDVP
CCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
YLPIDPQDVGCNYEAIVRINSQSGKGGIAYLLEKQYGIQIPKWMQPDFGTAVQEVTDRTG
CCCCCCHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
EELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPKGEFTF
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHEEEHHHCCCCCCEEE
KAKGNGPLDAFVRGMVSHTGIDFHVDEYTEHAIGHSSDAMAIAYIRLSFDDVALVCGSGI
EECCCCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCC
DSNISLASIKAIVSALNRYKKA
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]