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The map label for this gene is hmeA [H]

Identifier: 193211711

GI number: 193211711

Start: 41736

End: 42518

Strand: Direct

Name: hmeA [H]

Synonym: Cpar_0036

Alternate gene names: 193211711

Gene position: 41736-42518 (Clockwise)

Preceding gene: 193211710

Following gene: 193211712

Centisome position: 1.82

GC content: 62.84

Gene sequence:

>783_bases
ATGACCATGAACCAAAACCGTAGAGAGTTCCTGAAAAAGGCCGGACTCGGCGCGCTTGCCGGTCTCGGTGCGGCGGCGGG
GTTGTTCCCGGCGTTCGCCCTCGAACCGACCGATACCTCTAATTTCACCAAAAAGCCGACGCCCGGCAAGGTGCGCTGGG
GGATGCTGGTCGATACCCGCAAGTGCCTCGGCAACTGCACCGAGTGCGTTGTCCGCTGCCATCACGACCACAACGTGCCC
GATCTGAGCGGCACCAAGAACGAGGTCAAGTGGATATGGAAGAGCGGGTATGAAGAGGCCTTACCGACGGCGAGCCAGCA
GTTCCAGACCGACGAGGTCAAGCACCGCAAGGTGCTCACGCTCTGCAACCACTGCGAGGAGCCGCCCTGCACACGGGCCT
GCCCGACCGAGGCGACCTTCAAGCGCTGGGACGGCATCGTGTCGATGGACTACCACCGCTGCATCGGCTGCCGCTTCTGC
ATGGCCGCCTGCCCGTACGGTTCGCGCAGCTTCAACTGGGTGGATCCGCGCCCGCATGTCGCCGAGCTGAGCAATACCTA
TCCGACGCGAATGCGCGGCGTGGTGGAGAAGTGCAACTTCTGCTCCGAGCGCCTGGTCAAGGGCGAGCTTCCGGCGTGCG
TCGACAGCTGTCCGCAGAAAGCGCTCATCTTCGGCGACCTGAACGATCCGAACTCGGAGATCCGCCGGATGATCGCCGCC
AACGAAACCATGCAGCGCAAGCCGGAACTGGGCACGCTGCCGTCAGTCTTTTACATCATCTAA

Upstream 100 bases:

>100_bases
GCCACAACAACAATCCGATGTTCTGCTTTATGTGCCATCAGTACGCCGACGTCAAGCCGACCTGCTGGGACTGCCATCTG
TCGCCTATGGAGGTGCCGCA

Downstream 100 bases:

>100_bases
CCTGAAACCATGATCGAGAAAGCGCTCAAAGGGGGTCGCGGCTACTGGACATGGGTAGCCATCCTGCTCGCTGTCATCGG
CCTCGGCGTCACGGCGTACG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTMNQNRREFLKKAGLGALAGLGAAAGLFPAFALEPTDTSNFTKKPTPGKVRWGMLVDTRKCLGNCTECVVRCHHDHNVP
DLSGTKNEVKWIWKSGYEEALPTASQQFQTDEVKHRKVLTLCNHCEEPPCTRACPTEATFKRWDGIVSMDYHRCIGCRFC
MAACPYGSRSFNWVDPRPHVAELSNTYPTRMRGVVEKCNFCSERLVKGELPACVDSCPQKALIFGDLNDPNSEIRRMIAA
NETMQRKPELGTLPSVFYII

Sequences:

>Translated_260_residues
MTMNQNRREFLKKAGLGALAGLGAAAGLFPAFALEPTDTSNFTKKPTPGKVRWGMLVDTRKCLGNCTECVVRCHHDHNVP
DLSGTKNEVKWIWKSGYEEALPTASQQFQTDEVKHRKVLTLCNHCEEPPCTRACPTEATFKRWDGIVSMDYHRCIGCRFC
MAACPYGSRSFNWVDPRPHVAELSNTYPTRMRGVVEKCNFCSERLVKGELPACVDSCPQKALIFGDLNDPNSEIRRMIAA
NETMQRKPELGTLPSVFYII
>Mature_259_residues
TMNQNRREFLKKAGLGALAGLGAAAGLFPAFALEPTDTSNFTKKPTPGKVRWGMLVDTRKCLGNCTECVVRCHHDHNVPD
LSGTKNEVKWIWKSGYEEALPTASQQFQTDEVKHRKVLTLCNHCEEPPCTRACPTEATFKRWDGIVSMDYHRCIGCRFCM
AACPYGSRSFNWVDPRPHVAELSNTYPTRMRGVVEKCNFCSERLVKGELPACVDSCPQKALIFGDLNDPNSEIRRMIAAN
ETMQRKPELGTLPSVFYII

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=259, Percent_Identity=33.2046332046332, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789370, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787122, Length=192, Percent_Identity=34.375, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI226510944, Length=209, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=192, Percent_Identity=34.375, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790326, Length=178, Percent_Identity=30.3370786516854, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787749, Length=182, Percent_Identity=29.1208791208791, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787740, Length=108, Percent_Identity=35.1851851851852, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=108, Percent_Identity=35.1851851851852, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789079, Length=151, Percent_Identity=30.4635761589404, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789067, Length=110, Percent_Identity=37.2727272727273, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082179, Length=103, Percent_Identity=34.9514563106796, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082114, Length=183, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082180, Length=105, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29194; Mature: 29063

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNQNRREFLKKAGLGALAGLGAAAGLFPAFALEPTDTSNFTKKPTPGKVRWGMLVDTR
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEECHHHHHH
KCLGNCTECVVRCHHDHNVPDLSGTKNEVKWIWKSGYEEALPTASQQFQTDEVKHRKVLT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHH
LCNHCEEPPCTRACPTEATFKRWDGIVSMDYHRCIGCRFCMAACPYGSRSFNWVDPRPHV
HHHCCCCCCCCCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AELSNTYPTRMRGVVEKCNFCSERLVKGELPACVDSCPQKALIFGDLNDPNSEIRRMIAA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHH
NETMQRKPELGTLPSVFYII
HHHHHHCCCCCCCCCCHHCC
>Mature Secondary Structure 
TMNQNRREFLKKAGLGALAGLGAAAGLFPAFALEPTDTSNFTKKPTPGKVRWGMLVDTR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEECHHHHHH
KCLGNCTECVVRCHHDHNVPDLSGTKNEVKWIWKSGYEEALPTASQQFQTDEVKHRKVLT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHH
LCNHCEEPPCTRACPTEATFKRWDGIVSMDYHRCIGCRFCMAACPYGSRSFNWVDPRPHV
HHHCCCCCCCCCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AELSNTYPTRMRGVVEKCNFCSERLVKGELPACVDSCPQKALIFGDLNDPNSEIRRMIAA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHH
NETMQRKPELGTLPSVFYII
HHHHHHCCCCCCCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]