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The map label for this gene is metF [H]

Identifier: 193214260

GI number: 193214260

Start: 638728

End: 639612

Strand: Direct

Name: metF [H]

Synonym: Ctha_0541

Alternate gene names: 193214260

Gene position: 638728-639612 (Clockwise)

Preceding gene: 193214259

Following gene: 193214261

Centisome position: 19.39

GC content: 45.31

Gene sequence:

>885_bases
ATGATTATCAAAGACCTTTTACAACCCGATAAAACCCAATTTAGCTTTGAGTTTTATCCACCGAAAAAAGATGAGGAGTG
GGAAAAGCTCTTCTTTCACATATCGGAATTAATGCCGATGAATCCTGCGTATGTCAGCGTCACATACGGAGCAGGAGGCT
CTACGCGAGACAAAACCCACAACTTGGTTGTTCGAATTTCCAAAGAAACCAACATCCCTGTTGTCTCTCATTTGACTTGC
GTTGGCTCGAGCAAAAGCGAAATTCACACCATCCTTGAACGCTATGCTGAAAACGGCATTACCAATATTTTAGCCTTGCG
CGGCGATCCGCCAAAAGGATCTACCACTTTTGAAAAACCAGCCGATGGCTTCGCTTATGCAAATGAGTTGGTGGCGTTTA
TCAAAAAAAATTTCCCGCAAATAGGCGTTGGCGTGGCCGGTTTTCCTGAAGGCCATCCTGAAACCCTCAATCGATTGACA
GAAATCGACCATCTTAAAGAAAAAGTCGATGCCGGTGCGGACTACATTGTCACCCAGCTTTTCTTTGAGAATCGTGACTT
TTATGATTTCCGAGAACGCTGCGAAATCGCAGGGATTCATGTGCCGATTTGCGCTGGCATTATGCCAATCACAACCGACA
AAGGCATGCAAAGAATGGCAGAGCTTTCACCTGGCACACGATTCCCGGCGCCACTGCTTCGTGAAATATCCAAGGCTCAA
AATGATGAGGAAGTTTCAGCTATTGGACTCAATTGGGCGATTGCGCAAGTGCGCGACCTCATTGAAAATAAAACGCCAAA
TATTCATCTTTACACGCTCAATCATTCAAGGCCAACATTGAAAATTTTCGAGGCGCTCTCCGAGAAAAACCCCATCTCTT
CGTAG

Upstream 100 bases:

>100_bases
AATAATTTTCTTATGATATTGGCTCAGCGCACATGATGAATAATACTTTGTGCTGGCGTTAAAAAGCCTGAGAGTCACAC
TAAAAACTGGAAATTTACGC

Downstream 100 bases:

>100_bases
CAAACCTCCGGTTGAAATCCATTTCTAAAAAACCTTCTTATCTGTTTATAAGAAGGTTTTTTCTTTCCACCCTAATCGCA
ATCAGCATTTCCGCCATTAC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIIKDLLQPDKTQFSFEFYPPKKDEEWEKLFFHISELMPMNPAYVSVTYGAGGSTRDKTHNLVVRISKETNIPVVSHLTC
VGSSKSEIHTILERYAENGITNILALRGDPPKGSTTFEKPADGFAYANELVAFIKKNFPQIGVGVAGFPEGHPETLNRLT
EIDHLKEKVDAGADYIVTQLFFENRDFYDFRERCEIAGIHVPICAGIMPITTDKGMQRMAELSPGTRFPAPLLREISKAQ
NDEEVSAIGLNWAIAQVRDLIENKTPNIHLYTLNHSRPTLKIFEALSEKNPISS

Sequences:

>Translated_294_residues
MIIKDLLQPDKTQFSFEFYPPKKDEEWEKLFFHISELMPMNPAYVSVTYGAGGSTRDKTHNLVVRISKETNIPVVSHLTC
VGSSKSEIHTILERYAENGITNILALRGDPPKGSTTFEKPADGFAYANELVAFIKKNFPQIGVGVAGFPEGHPETLNRLT
EIDHLKEKVDAGADYIVTQLFFENRDFYDFRERCEIAGIHVPICAGIMPITTDKGMQRMAELSPGTRFPAPLLREISKAQ
NDEEVSAIGLNWAIAQVRDLIENKTPNIHLYTLNHSRPTLKIFEALSEKNPISS
>Mature_294_residues
MIIKDLLQPDKTQFSFEFYPPKKDEEWEKLFFHISELMPMNPAYVSVTYGAGGSTRDKTHNLVVRISKETNIPVVSHLTC
VGSSKSEIHTILERYAENGITNILALRGDPPKGSTTFEKPADGFAYANELVAFIKKNFPQIGVGVAGFPEGHPETLNRLT
EIDHLKEKVDAGADYIVTQLFFENRDFYDFRERCEIAGIHVPICAGIMPITTDKGMQRMAELSPGTRFPAPLLREISKAQ
NDEEVSAIGLNWAIAQVRDLIENKTPNIHLYTLNHSRPTLKIFEALSEKNPISS

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=291, Percent_Identity=32.9896907216495, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=36.996336996337, Blast_Score=180, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI25147441, Length=293, Percent_Identity=30.3754266211604, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25147436, Length=293, Percent_Identity=30.3754266211604, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6325234, Length=300, Percent_Identity=36.3333333333333, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=283, Percent_Identity=30.3886925795053, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24662658, Length=234, Percent_Identity=24.3589743589744, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33006; Mature: 33006

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKDLLQPDKTQFSFEFYPPKKDEEWEKLFFHISELMPMNPAYVSVTYGAGGSTRDKTH
CCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
NLVVRISKETNIPVVSHLTCVGSSKSEIHTILERYAENGITNILALRGDPPKGSTTFEKP
EEEEEEECCCCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
ADGFAYANELVAFIKKNFPQIGVGVAGFPEGHPETLNRLTEIDHLKEKVDAGADYIVTQL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FFENRDFYDFRERCEIAGIHVPICAGIMPITTDKGMQRMAELSPGTRFPAPLLREISKAQ
HHCCCCHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
NDEEVSAIGLNWAIAQVRDLIENKTPNIHLYTLNHSRPTLKIFEALSEKNPISS
CCCHHHHEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIIKDLLQPDKTQFSFEFYPPKKDEEWEKLFFHISELMPMNPAYVSVTYGAGGSTRDKTH
CCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
NLVVRISKETNIPVVSHLTCVGSSKSEIHTILERYAENGITNILALRGDPPKGSTTFEKP
EEEEEEECCCCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
ADGFAYANELVAFIKKNFPQIGVGVAGFPEGHPETLNRLTEIDHLKEKVDAGADYIVTQL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FFENRDFYDFRERCEIAGIHVPICAGIMPITTDKGMQRMAELSPGTRFPAPLLREISKAQ
HHCCCCHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
NDEEVSAIGLNWAIAQVRDLIENKTPNIHLYTLNHSRPTLKIFEALSEKNPISS
CCCHHHHEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]