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The map label for this gene is nusA [H]

Identifier: 193214185

GI number: 193214185

Start: 545268

End: 546863

Strand: Direct

Name: nusA [H]

Synonym: Ctha_0466

Alternate gene names: 193214185

Gene position: 545268-546863 (Clockwise)

Preceding gene: 193214184

Following gene: 193214186

Centisome position: 16.56

GC content: 44.36

Gene sequence:

>1596_bases
ATGGCTAAAAAGCAAGCAAAATTATCAGAGTCGGATGAGCGCAAAGCTCAAATTTTAAGAGCTTTTGGCGAGATTGAAAA
CTCAGACCGGCTTAAAGATAAAAAAGCGGACACCAATGCCGTTAAAATGGATATTGTCGATCTATTGCGCGACACCATTC
AGAAGCAACTTCGCAAGGATTATGATCCCGAGGTGGAAGCTCGTGTGATTATCAATCCAGATCGCGGCGACTTTGAGGTC
TACATTTTAAAACAGGTGGTTGAAGAAATAGATTTGCCAAGCATTGAAATCAGCCTTGAAGAAGCGCAAGAAATAGACGA
CTCGCTTGAGCTTGGGGATTTTTATGAAGTTGGGCCTATCCACCTTGAAGATTATTTGACCAGAAAGTCTATTCAAATTA
TCAAACAGGCCGTTCAGAAGAAAATGCGCGACGCTGAGCGCCAGGCGATTTATGAAGAATGCCTTGAAAAAGTAGGCGAA
GTGGTTTCAGCAGAGGTTCATCAGGTTCGCCAAAAAGAAGCGATTTTTACATACAATACCACAAAAGACCACAAAGTTGA
GCTGCTTTTGCCGAATACGGAAAAAATGCCGAAAGACAATCCGCGCAAAACGCCACGCATGCGGCTCTATGTAAAACGCA
TTGAACGAGAAAAGATCAGAGTTAAATTGGAAGATGGCACGGAAGTCGAGCGCGAACGCGAAGACGGGCCGATGAAAGTG
ATCGTGTCACGAACTGATGATCGTTTTCTCCATAAGCTTTTTGAAAGCGAAGTGCCAGAAATCTATGAAGGCTTGATTGT
AGTGAAAGGCATTGCGCGAGTTCCAGGTGAGCGGGCAAAAGTTTCCGTAGAATCCACCAGTTCAAGAATTGATCCGGTTG
GCGCGTGCGTTGGGCATCGCGGCAAACGCATTCAAAACATTGTTCGCGAGCTCAACAACGAAAATATTGACGTGATTTGC
TTTAGTGACGAGCCGCAGATTTATATTGCGCGCGCGCTTCAGCCTGCCAAAATTGACCCGATGACGGTTCATGTTGATCC
GAAAACTCGCAAATCGCGTGTGATGCTCAAACCCGACCAGATTAAATACGCGATCGGTAGAAATGGCAACAACATTCATC
TGGCTGAAAAACTGACCGGTTATGAAATAGAAATCTATCGCGATATTAGCGACAAAGCACTTGAAGATCCAGACGATATC
GATATTATTTCGTTCCGCGAAGAATTTGGCGACGATATGATTTATCAGTTGTTGGATAATGGGTTTGATACGGCTAAAAA
AATCCTTGAAGCTGGCGTAGACGCCATTGAAAAATCTTTGACCGCACCGCCGCGCCGCGAGGAAACTCTTGATTTTGGAC
GAAAATCACCAAGATCGCATCAGCCGCGTATACTGAGCGAAAGTGAACAGCGCTATTGGAGAAAAATAGCCGAAAGTATT
TATAAGACCATTAAAGAAGAGTTCGAAGAAGAAGATGGCTCTGCACAAGCATCAAAAAACAGCTTGGCCTCTGAGAACCA
AATAGAACAATCTCAAGCACCCGTAGCACCTTCTGACGTTCCTGACGAAGAAGATGAAAAAGCAGATTCGGAATAA

Upstream 100 bases:

>100_bases
AAATTCAAAAAGCAATTGTTCAAGTTGAATTTTAATTTGTTGCCATAGTGCAAGTAAACTTTCTTGTTGTCAAAGGCAAG
CAATTCATTTGAAAAGAGTT

Downstream 100 bases:

>100_bases
TGCACAGCTCTGCTTAAGTGGCCTCATAATGTGATGGAATGACGCTGCGGCATCTCATCATGATGTGTTGGGTGATAAGC
CCTGATTAATTGGTAATTCA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAKKQAKLSESDERKAQILRAFGEIENSDRLKDKKADTNAVKMDIVDLLRDTIQKQLRKDYDPEVEARVIINPDRGDFEV
YILKQVVEEIDLPSIEISLEEAQEIDDSLELGDFYEVGPIHLEDYLTRKSIQIIKQAVQKKMRDAERQAIYEECLEKVGE
VVSAEVHQVRQKEAIFTYNTTKDHKVELLLPNTEKMPKDNPRKTPRMRLYVKRIEREKIRVKLEDGTEVEREREDGPMKV
IVSRTDDRFLHKLFESEVPEIYEGLIVVKGIARVPGERAKVSVESTSSRIDPVGACVGHRGKRIQNIVRELNNENIDVIC
FSDEPQIYIARALQPAKIDPMTVHVDPKTRKSRVMLKPDQIKYAIGRNGNNIHLAEKLTGYEIEIYRDISDKALEDPDDI
DIISFREEFGDDMIYQLLDNGFDTAKKILEAGVDAIEKSLTAPPRREETLDFGRKSPRSHQPRILSESEQRYWRKIAESI
YKTIKEEFEEEDGSAQASKNSLASENQIEQSQAPVAPSDVPDEEDEKADSE

Sequences:

>Translated_531_residues
MAKKQAKLSESDERKAQILRAFGEIENSDRLKDKKADTNAVKMDIVDLLRDTIQKQLRKDYDPEVEARVIINPDRGDFEV
YILKQVVEEIDLPSIEISLEEAQEIDDSLELGDFYEVGPIHLEDYLTRKSIQIIKQAVQKKMRDAERQAIYEECLEKVGE
VVSAEVHQVRQKEAIFTYNTTKDHKVELLLPNTEKMPKDNPRKTPRMRLYVKRIEREKIRVKLEDGTEVEREREDGPMKV
IVSRTDDRFLHKLFESEVPEIYEGLIVVKGIARVPGERAKVSVESTSSRIDPVGACVGHRGKRIQNIVRELNNENIDVIC
FSDEPQIYIARALQPAKIDPMTVHVDPKTRKSRVMLKPDQIKYAIGRNGNNIHLAEKLTGYEIEIYRDISDKALEDPDDI
DIISFREEFGDDMIYQLLDNGFDTAKKILEAGVDAIEKSLTAPPRREETLDFGRKSPRSHQPRILSESEQRYWRKIAESI
YKTIKEEFEEEDGSAQASKNSLASENQIEQSQAPVAPSDVPDEEDEKADSE
>Mature_530_residues
AKKQAKLSESDERKAQILRAFGEIENSDRLKDKKADTNAVKMDIVDLLRDTIQKQLRKDYDPEVEARVIINPDRGDFEVY
ILKQVVEEIDLPSIEISLEEAQEIDDSLELGDFYEVGPIHLEDYLTRKSIQIIKQAVQKKMRDAERQAIYEECLEKVGEV
VSAEVHQVRQKEAIFTYNTTKDHKVELLLPNTEKMPKDNPRKTPRMRLYVKRIEREKIRVKLEDGTEVEREREDGPMKVI
VSRTDDRFLHKLFESEVPEIYEGLIVVKGIARVPGERAKVSVESTSSRIDPVGACVGHRGKRIQNIVRELNNENIDVICF
SDEPQIYIARALQPAKIDPMTVHVDPKTRKSRVMLKPDQIKYAIGRNGNNIHLAEKLTGYEIEIYRDISDKALEDPDDID
IISFREEFGDDMIYQLLDNGFDTAKKILEAGVDAIEKSLTAPPRREETLDFGRKSPRSHQPRILSESEQRYWRKIAESIY
KTIKEEFEEEDGSAQASKNSLASENQIEQSQAPVAPSDVPDEEDEKADSE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=393, Percent_Identity=31.2977099236641, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 61061; Mature: 60929

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQAKLSESDERKAQILRAFGEIENSDRLKDKKADTNAVKMDIVDLLRDTIQKQLRKD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
YDPEVEARVIINPDRGDFEVYILKQVVEEIDLPSIEISLEEAQEIDDSLELGDFYEVGPI
CCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
HLEDYLTRKSIQIIKQAVQKKMRDAERQAIYEECLEKVGEVVSAEVHQVRQKEAIFTYNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
TKDHKVELLLPNTEKMPKDNPRKTPRMRLYVKRIEREKIRVKLEDGTEVEREREDGPMKV
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCEEE
IVSRTDDRFLHKLFESEVPEIYEGLIVVKGIARVPGERAKVSVESTSSRIDPVGACVGHR
EEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCHHHHHHCCC
GKRIQNIVRELNNENIDVICFSDEPQIYIARALQPAKIDPMTVHVDPKTRKSRVMLKPDQ
CHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEECCCC
IKYAIGRNGNNIHLAEKLTGYEIEIYRDISDKALEDPDDIDIISFREEFGDDMIYQLLDN
EEEEECCCCCEEEEEECCCCCEEEEEECCCHHCCCCCCCCCEEHHHHHHCHHHHHHHHHC
GFDTAKKILEAGVDAIEKSLTAPPRREETLDFGRKSPRSHQPRILSESEQRYWRKIAESI
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YKTIKEEFEEEDGSAQASKNSLASENQIEQSQAPVAPSDVPDEEDEKADSE
HHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKQAKLSESDERKAQILRAFGEIENSDRLKDKKADTNAVKMDIVDLLRDTIQKQLRKD
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
YDPEVEARVIINPDRGDFEVYILKQVVEEIDLPSIEISLEEAQEIDDSLELGDFYEVGPI
CCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
HLEDYLTRKSIQIIKQAVQKKMRDAERQAIYEECLEKVGEVVSAEVHQVRQKEAIFTYNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
TKDHKVELLLPNTEKMPKDNPRKTPRMRLYVKRIEREKIRVKLEDGTEVEREREDGPMKV
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCEEE
IVSRTDDRFLHKLFESEVPEIYEGLIVVKGIARVPGERAKVSVESTSSRIDPVGACVGHR
EEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCHHHHHHCCC
GKRIQNIVRELNNENIDVICFSDEPQIYIARALQPAKIDPMTVHVDPKTRKSRVMLKPDQ
CHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEECCCC
IKYAIGRNGNNIHLAEKLTGYEIEIYRDISDKALEDPDDIDIISFREEFGDDMIYQLLDN
EEEEECCCCCEEEEEECCCCCEEEEEECCCHHCCCCCCCCCEEHHHHHHCHHHHHHHHHC
GFDTAKKILEAGVDAIEKSLTAPPRREETLDFGRKSPRSHQPRILSESEQRYWRKIAESI
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YKTIKEEFEEEDGSAQASKNSLASENQIEQSQAPVAPSDVPDEEDEKADSE
HHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]