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The map label for this gene is lysS [H]

Identifier: 193213961

GI number: 193213961

Start: 288734

End: 290287

Strand: Direct

Name: lysS [H]

Synonym: Ctha_0242

Alternate gene names: 193213961

Gene position: 288734-290287 (Clockwise)

Preceding gene: 193213960

Following gene: 193213962

Centisome position: 8.77

GC content: 49.29

Gene sequence:

>1554_bases
ATGTCGAATCAGCCAACCAAGCCGGTGCAGTCCGAAGAAAACGCAGAACACTTGAGCCAGCAAGCTCAGGAAGAATTAAA
TGACCAAATGCTGCGCCGCCGCGAAGAGCTTGAAAAACTTCGCGAAATGGATGTAAATCCGTATCCATATAGTTTTGAAG
TCACTCACACAGGCAAACAAATCATAGAGAATTTTAAAGACGACGAAAAAGCAGCGGTCGCCATTGCCGGCAGAATTATG
TCCATTCGCAAAATGGGCAAAGCCTCGTTTTTCCATGTGCAAGATGCCTCCGGCAGAATTCAAGTTTACATCAAAAAGGA
CGAAGTCGGCGAAACGGCTTATCAGGTTTTCAAACTTTTAGACATCGGCGATATTGTTGGCGTCAAAGGCTTTAGCTTCC
GCACAAAAACCGGTGAGGTTTCCGTTCATGCCGAAGCATGTGAATTGCTCTCCAAATCGCTGCGCCCGATGCCTATTGCC
AAACAAAAAGAAGTCGACGGCAAAACCGTTACCTACGACGCGTTCAGCGACAAAGAACTGCGCTATCGCCAACGCTATGT
AGATTTGATTGTCAATCCTGAGGTGAAAACCGTGTTTTTGAAGCGGACAAAAATGATAAATTCCATCCGCAATTTTCTCA
ACGAACGCGGCTACTTGGAAGTCGAAACGCCAATTCTTCAGCCGATTTACGGCGGCGCGTCGGCCAGGCCGTTCGTGACG
CATCACAACACGCTCGACATGCCGCTTTATTTGCGCATCGCCAACGAGCTTTATTTGAAGCGCCTCATCGTCGGCGGCTT
CGACGGCGTTTATGAGTTCGCGAAGGATTTCAGAAACGAAGGCATGGACAGATTCCACAACCCGGAATTCACGCAAATCG
AGCTGTATGTCGCTTATAAAGATTATGCGTGGATGATGAACTTGGTTGAGGAAATGCTTTCAAAAACGGCGGAAGCCGTG
AACGGCTCAACTGTCGTAGAATTTGCCGGCCATAAAATCGACTTGAAACCACCGTTCAAGCGCATGACCATCACGGATTC
GATTAAGGAATACACCGGCAAAGACATCGATGGCAAGTCGGAAGCCGAACTTCGCCAAATCGCAAAAGAGTTGGGCATTG
AGATTGAGGCAAATTTAGGCAGCTCGAAAATCATCGATGAAATTTTCGGCGAATATGTCGAGCCGAAGCTCATCGACCCG
ACATTTATCATGGACTATCCGGTGGAAATGTCGCCGCTGGCGAAAAAGCATCGCGAGAAACCCGGATTTGTGGAGCGATT
TGAACTCATTGCCGCTGGAAAAGAAATCTGCAATTCCTTCTCGGAACTCAACGATCCGCTCGACCAGCGCGCCAGATTGG
AAGAGCAAGCCAGCCTGCGTGCCAGAGGCGACGACGAAGCGATGGCGGTCGACGAGGATTTCCTTCGCGCGTTGGAATAC
GGCATGCCGCCTTGCGCCGGTCTCGGCATCGGCATCGACCGTTTGGCCATGATTTTAACTGGACAAGAGTCTATTCGCGA
CGTGCTCTTTTTTCCGCAAATGAAGCCAGAATAA

Upstream 100 bases:

>100_bases
GGCTAAAACTTTTCAAATCGTCCCATTTATGAGCGGATTTTTGGTAAATTTCCGCTCATTTATTTTCATCAATTGCAAAA
TTTCAGATAAAAAGCCGTTT

Downstream 100 bases:

>100_bases
CCGGCTTTTTGCGCATCGCAATTAACCGCTAAGATTGAAAACTTAGCGGTTTTTTTATTTTCGTCGATTTTTTCTCAAAA
ATATTCCGCTAACCTGCTTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSNQPTKPVQSEENAEHLSQQAQEELNDQMLRRREELEKLREMDVNPYPYSFEVTHTGKQIIENFKDDEKAAVAIAGRIM
SIRKMGKASFFHVQDASGRIQVYIKKDEVGETAYQVFKLLDIGDIVGVKGFSFRTKTGEVSVHAEACELLSKSLRPMPIA
KQKEVDGKTVTYDAFSDKELRYRQRYVDLIVNPEVKTVFLKRTKMINSIRNFLNERGYLEVETPILQPIYGGASARPFVT
HHNTLDMPLYLRIANELYLKRLIVGGFDGVYEFAKDFRNEGMDRFHNPEFTQIELYVAYKDYAWMMNLVEEMLSKTAEAV
NGSTVVEFAGHKIDLKPPFKRMTITDSIKEYTGKDIDGKSEAELRQIAKELGIEIEANLGSSKIIDEIFGEYVEPKLIDP
TFIMDYPVEMSPLAKKHREKPGFVERFELIAAGKEICNSFSELNDPLDQRARLEEQASLRARGDDEAMAVDEDFLRALEY
GMPPCAGLGIGIDRLAMILTGQESIRDVLFFPQMKPE

Sequences:

>Translated_517_residues
MSNQPTKPVQSEENAEHLSQQAQEELNDQMLRRREELEKLREMDVNPYPYSFEVTHTGKQIIENFKDDEKAAVAIAGRIM
SIRKMGKASFFHVQDASGRIQVYIKKDEVGETAYQVFKLLDIGDIVGVKGFSFRTKTGEVSVHAEACELLSKSLRPMPIA
KQKEVDGKTVTYDAFSDKELRYRQRYVDLIVNPEVKTVFLKRTKMINSIRNFLNERGYLEVETPILQPIYGGASARPFVT
HHNTLDMPLYLRIANELYLKRLIVGGFDGVYEFAKDFRNEGMDRFHNPEFTQIELYVAYKDYAWMMNLVEEMLSKTAEAV
NGSTVVEFAGHKIDLKPPFKRMTITDSIKEYTGKDIDGKSEAELRQIAKELGIEIEANLGSSKIIDEIFGEYVEPKLIDP
TFIMDYPVEMSPLAKKHREKPGFVERFELIAAGKEICNSFSELNDPLDQRARLEEQASLRARGDDEAMAVDEDFLRALEY
GMPPCAGLGIGIDRLAMILTGQESIRDVLFFPQMKPE
>Mature_516_residues
SNQPTKPVQSEENAEHLSQQAQEELNDQMLRRREELEKLREMDVNPYPYSFEVTHTGKQIIENFKDDEKAAVAIAGRIMS
IRKMGKASFFHVQDASGRIQVYIKKDEVGETAYQVFKLLDIGDIVGVKGFSFRTKTGEVSVHAEACELLSKSLRPMPIAK
QKEVDGKTVTYDAFSDKELRYRQRYVDLIVNPEVKTVFLKRTKMINSIRNFLNERGYLEVETPILQPIYGGASARPFVTH
HNTLDMPLYLRIANELYLKRLIVGGFDGVYEFAKDFRNEGMDRFHNPEFTQIELYVAYKDYAWMMNLVEEMLSKTAEAVN
GSTVVEFAGHKIDLKPPFKRMTITDSIKEYTGKDIDGKSEAELRQIAKELGIEIEANLGSSKIIDEIFGEYVEPKLIDPT
FIMDYPVEMSPLAKKHREKPGFVERFELIAAGKEICNSFSELNDPLDQRARLEEQASLRARGDDEAMAVDEDFLRALEYG
MPPCAGLGIGIDRLAMILTGQESIRDVLFFPQMKPE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=41.1417322834646, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=41.1417322834646, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI40789249, Length=289, Percent_Identity=23.8754325259516, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790571, Length=505, Percent_Identity=46.7326732673267, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1789256, Length=518, Percent_Identity=45.5598455598456, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=30.7453416149068, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788173, Length=325, Percent_Identity=22.4615384615385, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=41.8650793650794, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=41.8650793650794, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71994340, Length=508, Percent_Identity=41.7322834645669, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551876, Length=475, Percent_Identity=22.5263157894737, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=308, Percent_Identity=24.6753246753247, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=515, Percent_Identity=40.7766990291262, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=496, Percent_Identity=28.6290322580645, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=481, Percent_Identity=22.8690228690229, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=461, Percent_Identity=23.644251626898, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640849, Length=511, Percent_Identity=42.2700587084149, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640851, Length=511, Percent_Identity=42.2700587084149, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17136276, Length=474, Percent_Identity=22.5738396624473, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24584738, Length=311, Percent_Identity=26.3665594855305, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922416, Length=475, Percent_Identity=21.8947368421053, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 59038; Mature: 58907

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQPTKPVQSEENAEHLSQQAQEELNDQMLRRREELEKLREMDVNPYPYSFEVTHTGKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHH
IIENFKDDEKAAVAIAGRIMSIRKMGKASFFHVQDASGRIQVYIKKDEVGETAYQVFKLL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHH
DIGDIVGVKGFSFRTKTGEVSVHAEACELLSKSLRPMPIAKQKEVDGKTVTYDAFSDKEL
HHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHH
RYRQRYVDLIVNPEVKTVFLKRTKMINSIRNFLNERGYLEVETPILQPIYGGASARPFVT
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEE
HHNTLDMPLYLRIANELYLKRLIVGGFDGVYEFAKDFRNEGMDRFHNPEFTQIELYVAYK
ECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEH
DYAWMMNLVEEMLSKTAEAVNGSTVVEFAGHKIDLKPPFKRMTITDSIKEYTGKDIDGKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHEEHHHHHHHHCCCCCCCCC
EAELRQIAKELGIEIEANLGSSKIIDEIFGEYVEPKLIDPTFIMDYPVEMSPLAKKHREK
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCC
PGFVERFELIAAGKEICNSFSELNDPLDQRARLEEQASLRARGDDEAMAVDEDFLRALEY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
GMPPCAGLGIGIDRLAMILTGQESIRDVLFFPQMKPE
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNQPTKPVQSEENAEHLSQQAQEELNDQMLRRREELEKLREMDVNPYPYSFEVTHTGKQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHH
IIENFKDDEKAAVAIAGRIMSIRKMGKASFFHVQDASGRIQVYIKKDEVGETAYQVFKLL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHH
DIGDIVGVKGFSFRTKTGEVSVHAEACELLSKSLRPMPIAKQKEVDGKTVTYDAFSDKEL
HHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHH
RYRQRYVDLIVNPEVKTVFLKRTKMINSIRNFLNERGYLEVETPILQPIYGGASARPFVT
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEE
HHNTLDMPLYLRIANELYLKRLIVGGFDGVYEFAKDFRNEGMDRFHNPEFTQIELYVAYK
ECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEH
DYAWMMNLVEEMLSKTAEAVNGSTVVEFAGHKIDLKPPFKRMTITDSIKEYTGKDIDGKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHEEHHHHHHHHCCCCCCCCC
EAELRQIAKELGIEIEANLGSSKIIDEIFGEYVEPKLIDPTFIMDYPVEMSPLAKKHREK
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCC
PGFVERFELIAAGKEICNSFSELNDPLDQRARLEEQASLRARGDDEAMAVDEDFLRALEY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
GMPPCAGLGIGIDRLAMILTGQESIRDVLFFPQMKPE
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA