Click here to switch to the map view.

The map label for this gene is nadB1 [H]

Identifier: 193213932

GI number: 193213932

Start: 248454

End: 250046

Strand: Direct

Name: nadB1 [H]

Synonym: Ctha_0213

Alternate gene names: 193213932

Gene position: 248454-250046 (Clockwise)

Preceding gene: 193213931

Following gene: 193213935

Centisome position: 7.54

GC content: 47.65

Gene sequence:

>1593_bases
ATGACCGAAGAACTTAAAACTGATATTTTGGTAATCGGCAGCGGAATCGCCGGACTTTATTTTGCATTGAAAGCAGCGGA
GCTTGGCGAGGTTATCCTCATCACGAAAAAAGAACGCTCTGATTCGAATACAAACTGGGCACAAGGCGGAATTGCCGCTG
TTTTGGATGAAAATGACACATTTGACGCTCACATCGCCGATACGCTGAACGCGGGCGTTGGGCTTTGCAATCAGGAAGCC
GTTGAAGTGATGGTGAAAGAAGGCCCCGCACATATTTCAAAGTTGATTGAAATTGGTACGAACTTCACCAAAAAATCGGG
AAAGCTCGACTTAGGACGCGAAGGCGGGCATTCCTACAACCGAATCGTACACGCTGCAGATTTAACCGGCCACGAAGTGG
AACAAACCTTGCTTTCGAAAGTGGCTGAATCCCCCAACATTCAATTGCTTGAACATCATTTTGGAATTGAACTGATTACC
GAGCATCATCTCAAATGCAAAACCAACGATATTACTTGCTACGGGGTCTATGCGCTGGATTCAAAATCAAGGAAAATCAA
AAAGATTTTGGCTAAAGCCACCATGCTCGCTACCGGCGGACTTGGCCAAGTGTATCAACACACTACGAACCCAAGCATCG
CAACAGGCGACGGTATCGCCATGTCGTATCGTGCCGGCGCAGAAATTCACAACATGGAGTTCATCCAATTTCACCCAACC
ACGCTTTTTCATCCAAATGCCGATTCGTTTTTGATAAGTGAAGCGGTTCGCGGTTTTGGCGGTGTGCTGCGTCTGAAAAA
TGGCGAAGAATTTATGCACAAATACGACGAGCGAAAATCGCTGGCGCCGCGCGACATTGTCGCCCGTGCGATCGATAACG
AAATCAAAAAATCGGGTGAGGAATGCGTTTTCTTAGATGTCACGCATTTGGATTCGGAAAGCGTTACCCAGCATTTTCCC
AACATTTACAACACTTGCCTGGAATACGGCATCGATATTACCAAAGAGATGATTCCGGTTGTTCCGGCGGCGCATTATTC
TTGCGGCGGCGTAGCGACGGATTTAATCGGGCGAACCACCATCAATCGCTTGTATGCTTGCGGCGAAGCTGCCAGTGTGG
GTGTGCATGGCGCAAACCGTTTGGCGAGCAATTCGCTGCTCGAGGCACTCGTTTTTTCTCATCGCGCTTTTTTAGACATG
AAAGAACGACTTCAATATATCGAAAATAATATTTCCTTCCCGGATTGGGACGATAGCGGCACGGAAAATGCCGAAGAGTG
GATTTTGGTTTCGCACAACCGCAAAGAAGCGCAGCAGGTTATGAACGATTATGTTGGGATTGTTCGCAGCGACTTGAGGC
TTGATAGAGCCAGCCGGCGCATGGAGTTTTTGAAAGAGGAAACGGAGGTCTTTTACAAGAAAACCAAAATTACGCCGCAA
GTTCTCGAGTTGAGAAACGCAATTAAGGTTTCATCGCTGGTTATCGAAAGTGCGCTCAAACGGCGAGAATCGCGCGGCTT
GCACTACACAACCGATTATCCGTATGTCGACGATAAGCACTTCCTAAAAAACACCGTGCTGCGATCGTTTTGA

Upstream 100 bases:

>100_bases
TTTGAATTTTTTTTTGCAAGAATGTATTAAAAAGGCACGCAAAACAAGATTCTCGTGCTAAATTAACTTTTTTAACTCTG
GTTTAGTGCTTGTTGAGACA

Downstream 100 bases:

>100_bases
GCAAAGCAATTTTTGCGCTAAGTGCGCTGAATGAAATAAAAAAGCCAGCTAAAAAGCTGGCTTTTTTGTTGAGCAAAACT
GTTAGGATTAAGCCACTTCA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTEELKTDILVIGSGIAGLYFALKAAELGEVILITKKERSDSNTNWAQGGIAAVLDENDTFDAHIADTLNAGVGLCNQEA
VEVMVKEGPAHISKLIEIGTNFTKKSGKLDLGREGGHSYNRIVHAADLTGHEVEQTLLSKVAESPNIQLLEHHFGIELIT
EHHLKCKTNDITCYGVYALDSKSRKIKKILAKATMLATGGLGQVYQHTTNPSIATGDGIAMSYRAGAEIHNMEFIQFHPT
TLFHPNADSFLISEAVRGFGGVLRLKNGEEFMHKYDERKSLAPRDIVARAIDNEIKKSGEECVFLDVTHLDSESVTQHFP
NIYNTCLEYGIDITKEMIPVVPAAHYSCGGVATDLIGRTTINRLYACGEAASVGVHGANRLASNSLLEALVFSHRAFLDM
KERLQYIENNISFPDWDDSGTENAEEWILVSHNRKEAQQVMNDYVGIVRSDLRLDRASRRMEFLKEETEVFYKKTKITPQ
VLELRNAIKVSSLVIESALKRRESRGLHYTTDYPYVDDKHFLKNTVLRSF

Sequences:

>Translated_530_residues
MTEELKTDILVIGSGIAGLYFALKAAELGEVILITKKERSDSNTNWAQGGIAAVLDENDTFDAHIADTLNAGVGLCNQEA
VEVMVKEGPAHISKLIEIGTNFTKKSGKLDLGREGGHSYNRIVHAADLTGHEVEQTLLSKVAESPNIQLLEHHFGIELIT
EHHLKCKTNDITCYGVYALDSKSRKIKKILAKATMLATGGLGQVYQHTTNPSIATGDGIAMSYRAGAEIHNMEFIQFHPT
TLFHPNADSFLISEAVRGFGGVLRLKNGEEFMHKYDERKSLAPRDIVARAIDNEIKKSGEECVFLDVTHLDSESVTQHFP
NIYNTCLEYGIDITKEMIPVVPAAHYSCGGVATDLIGRTTINRLYACGEAASVGVHGANRLASNSLLEALVFSHRAFLDM
KERLQYIENNISFPDWDDSGTENAEEWILVSHNRKEAQQVMNDYVGIVRSDLRLDRASRRMEFLKEETEVFYKKTKITPQ
VLELRNAIKVSSLVIESALKRRESRGLHYTTDYPYVDDKHFLKNTVLRSF
>Mature_529_residues
TEELKTDILVIGSGIAGLYFALKAAELGEVILITKKERSDSNTNWAQGGIAAVLDENDTFDAHIADTLNAGVGLCNQEAV
EVMVKEGPAHISKLIEIGTNFTKKSGKLDLGREGGHSYNRIVHAADLTGHEVEQTLLSKVAESPNIQLLEHHFGIELITE
HHLKCKTNDITCYGVYALDSKSRKIKKILAKATMLATGGLGQVYQHTTNPSIATGDGIAMSYRAGAEIHNMEFIQFHPTT
LFHPNADSFLISEAVRGFGGVLRLKNGEEFMHKYDERKSLAPRDIVARAIDNEIKKSGEECVFLDVTHLDSESVTQHFPN
IYNTCLEYGIDITKEMIPVVPAAHYSCGGVATDLIGRTTINRLYACGEAASVGVHGANRLASNSLLEALVFSHRAFLDMK
ERLQYIENNISFPDWDDSGTENAEEWILVSHNRKEAQQVMNDYVGIVRSDLRLDRASRRMEFLKEETEVFYKKTKITPQV
LELRNAIKVSSLVIESALKRRESRGLHYTTDYPYVDDKHFLKNTVLRSF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=33.0152671755725, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788928, Length=516, Percent_Identity=47.093023255814, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1790597, Length=541, Percent_Identity=32.3475046210721, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1786942, Length=574, Percent_Identity=32.5783972125435, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=31.4126394052045, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17505833, Length=526, Percent_Identity=32.6996197718631, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71986328, Length=458, Percent_Identity=25.5458515283843, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=570, Percent_Identity=33.6842105263158, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6322416, Length=548, Percent_Identity=32.2992700729927, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322511, Length=487, Percent_Identity=26.4887063655031, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320788, Length=478, Percent_Identity=27.1966527196653, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137288, Length=542, Percent_Identity=32.2878228782288, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24655642, Length=542, Percent_Identity=32.2878228782288, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24655647, Length=542, Percent_Identity=32.2878228782288, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24663005, Length=562, Percent_Identity=31.4946619217082, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59042; Mature: 58910

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEELKTDILVIGSGIAGLYFALKAAELGEVILITKKERSDSNTNWAQGGIAAVLDENDT
CCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
FDAHIADTLNAGVGLCNQEAVEVMVKEGPAHISKLIEIGTNFTKKSGKLDLGREGGHSYN
CCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
RIVHAADLTGHEVEQTLLSKVAESPNIQLLEHHFGIELITEHHLKCKTNDITCYGVYALD
HEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHCCEEEEECCCCEEECCCEEEEEEEEEC
SKSRKIKKILAKATMLATGGLGQVYQHTTNPSIATGDGIAMSYRAGAEIHNMEFIQFHPT
CCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCEECEEEEEECCC
TLFHPNADSFLISEAVRGFGGVLRLKNGEEFMHKYDERKSLAPRDIVARAIDNEIKKSGE
EEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
ECVFLDVTHLDSESVTQHFPNIYNTCLEYGIDITKEMIPVVPAAHYSCGGVATDLIGRTT
CEEEEEEEECCCHHHHHHCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
INRLYACGEAASVGVHGANRLASNSLLEALVFSHRAFLDMKERLQYIENNISFPDWDDSG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENAEEWILVSHNRKEAQQVMNDYVGIVRSDLRLDRASRRMEFLKEETEVFYKKTKITPQ
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLELRNAIKVSSLVIESALKRRESRGLHYTTDYPYVDDKHFLKNTVLRSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TEELKTDILVIGSGIAGLYFALKAAELGEVILITKKERSDSNTNWAQGGIAAVLDENDT
CCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
FDAHIADTLNAGVGLCNQEAVEVMVKEGPAHISKLIEIGTNFTKKSGKLDLGREGGHSYN
CCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
RIVHAADLTGHEVEQTLLSKVAESPNIQLLEHHFGIELITEHHLKCKTNDITCYGVYALD
HEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHCCEEEEECCCCEEECCCEEEEEEEEEC
SKSRKIKKILAKATMLATGGLGQVYQHTTNPSIATGDGIAMSYRAGAEIHNMEFIQFHPT
CCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCEECEEEEEECCC
TLFHPNADSFLISEAVRGFGGVLRLKNGEEFMHKYDERKSLAPRDIVARAIDNEIKKSGE
EEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
ECVFLDVTHLDSESVTQHFPNIYNTCLEYGIDITKEMIPVVPAAHYSCGGVATDLIGRTT
CEEEEEEEECCCHHHHHHCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
INRLYACGEAASVGVHGANRLASNSLLEALVFSHRAFLDMKERLQYIENNISFPDWDDSG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENAEEWILVSHNRKEAQQVMNDYVGIVRSDLRLDRASRRMEFLKEETEVFYKKTKITPQ
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLELRNAIKVSSLVIESALKRRESRGLHYTTDYPYVDDKHFLKNTVLRSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]