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The map label for this gene is rpoN [H]

Identifier: 193213899

GI number: 193213899

Start: 210966

End: 212519

Strand: Direct

Name: rpoN [H]

Synonym: Ctha_0180

Alternate gene names: 193213899

Gene position: 210966-212519 (Clockwise)

Preceding gene: 193213898

Following gene: 193213900

Centisome position: 6.41

GC content: 42.66

Gene sequence:

>1554_bases
ATGAACACCCAATCACTATCGCAACGACAAGTGCAAACCATGCGGCTTAGCCCGCAGCAACTTTTATACACAAAGCTTTT
GCAACTTCCGTTAATTCAGCTTGAACAGCGCGTCAAGCAGGAATTGGAGGAAAATCCCATGCTGGAGGAATTTTCTGAAT
CGGATGAGGAAGCTGGCCAACCAGAAGAAATGGCCACGGACCTTTCGCAAAATAATGATGATGAAAATAAGCTGAACGAA
AAATCGGAGCAAAACGAGGAGGATCTCTACATCGCCGAAAAACCAACGAAAGACGAACCGCTTCCGAAAGACGATGACTA
CACCAAAGAGTTTGATCAAGAAATTGAGGAATATTTTCCTGATGGCGATGAAGAAGGCTTCAATGTGCCGTCTTTCCGCG
AAGAACCATCCGACGAAAGCTACTTTCAACCGGTTTTTCATGAGAGCTTTACCGAGCGCGTTTTAAACGATTTGCGCTAT
CAAGATGTCACAGAAAAAGAATTAGCAATTGCTGAGGAAATTGTCGGAAATATTGATGAAGATGGCTACCTTCGCTGCCC
AATTGAAGTGATTGCCGATGGCCTGATGACAACCGGAATTGAAGTTACTGAAGCTGAAATTTTGAAAGTGCTGGAAAAAA
TCTGGTACATCGATCCGGTTGGCATTGGCGCAAGAAATTTACAGGAATGTTTGCTGATTCAACTTGAAGCCAAATATCAA
CGAACGGAAGATATCGACGACACGCTCTATGAACAAGCCAAGCTTATTCTGAATAAATATTACCACGAGTTCACAATGAA
ACACTTTGAGAAGCTAATGACCCTTCTCAGTATCTCACCAGAAGAACTCACGCAAGCACTTTTGCTCATTCAAAGCTTTA
ATCCAAAACCGGGCGGCGATGTTTCGGCTTCAAGCAATTACATAATCCCTGATTTTATTGTTACTTTTGATGGGAAAAAT
TTGGTGGCCTTTTCCAATGAACGAAATTCTTTTCAGATCCGAATTTCGGAACAATACAAACCGTTGCTTGAAGACAAGTC
TCAGCCAAAAGGAGCAAAAGAGTTTGTCCGCAACAAATACGAAGCAGCCAAAAATTTCATGACGGCCATTGAAATGCGTC
GCCAAACCTTGCAGCGCGTGATGAACGCTCTTTTAAATCTGCAATATGAATTTTTCACCATTGGGCCAAACCGGTTGAAA
CCGATGATTTTAAAAGATGTGGCTGAAGTGGCTGGCGTCGATATTTCAACGGTGAGCCGCGTGGTAAATGGAAAATATGT
GCAAGCGCACAAGGGCATTTTGGAATTGAAATATTTCTTCAGCACAGGACTCGAGACCGAATCGGGCGATGAAATTTCCA
ACAAGCTGATTAAAGAAAAAATCAAATCGTTTATTGAAAAAGAAGATACACGCAAGCCGCTGAGCGACGACAAAATCTCC
GAGATGCTTCAGCAAAACGGATTCAAGGTGGCACGAAGAACCGTCGCCAAATATCGCGAGCAAATGAATATTCCAGTAGC
TCGCCTTCGAAAACAAATACAGTCTCTTAAATAA

Upstream 100 bases:

>100_bases
AGAAGTTTGTCATCAGTTTAGCGAGCAATAACTCGAATTTTGTTACAAAGTTGCTCCTATGGAGCGGTGTCGATTAATTG
TTTAGACAGCAGGAAGAGAT

Downstream 100 bases:

>100_bases
TTCAAACCAGTTTGTGTAGTGAGTAACGAGAAACCAATTATGCGCGCGACAACCGTCATTGGTGTTTTGCGAAATGGAAA
AGCGGCGCTTGGCAGCGACG

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MNTQSLSQRQVQTMRLSPQQLLYTKLLQLPLIQLEQRVKQELEENPMLEEFSESDEEAGQPEEMATDLSQNNDDENKLNE
KSEQNEEDLYIAEKPTKDEPLPKDDDYTKEFDQEIEEYFPDGDEEGFNVPSFREEPSDESYFQPVFHESFTERVLNDLRY
QDVTEKELAIAEEIVGNIDEDGYLRCPIEVIADGLMTTGIEVTEAEILKVLEKIWYIDPVGIGARNLQECLLIQLEAKYQ
RTEDIDDTLYEQAKLILNKYYHEFTMKHFEKLMTLLSISPEELTQALLLIQSFNPKPGGDVSASSNYIIPDFIVTFDGKN
LVAFSNERNSFQIRISEQYKPLLEDKSQPKGAKEFVRNKYEAAKNFMTAIEMRRQTLQRVMNALLNLQYEFFTIGPNRLK
PMILKDVAEVAGVDISTVSRVVNGKYVQAHKGILELKYFFSTGLETESGDEISNKLIKEKIKSFIEKEDTRKPLSDDKIS
EMLQQNGFKVARRTVAKYREQMNIPVARLRKQIQSLK

Sequences:

>Translated_517_residues
MNTQSLSQRQVQTMRLSPQQLLYTKLLQLPLIQLEQRVKQELEENPMLEEFSESDEEAGQPEEMATDLSQNNDDENKLNE
KSEQNEEDLYIAEKPTKDEPLPKDDDYTKEFDQEIEEYFPDGDEEGFNVPSFREEPSDESYFQPVFHESFTERVLNDLRY
QDVTEKELAIAEEIVGNIDEDGYLRCPIEVIADGLMTTGIEVTEAEILKVLEKIWYIDPVGIGARNLQECLLIQLEAKYQ
RTEDIDDTLYEQAKLILNKYYHEFTMKHFEKLMTLLSISPEELTQALLLIQSFNPKPGGDVSASSNYIIPDFIVTFDGKN
LVAFSNERNSFQIRISEQYKPLLEDKSQPKGAKEFVRNKYEAAKNFMTAIEMRRQTLQRVMNALLNLQYEFFTIGPNRLK
PMILKDVAEVAGVDISTVSRVVNGKYVQAHKGILELKYFFSTGLETESGDEISNKLIKEKIKSFIEKEDTRKPLSDDKIS
EMLQQNGFKVARRTVAKYREQMNIPVARLRKQIQSLK
>Mature_517_residues
MNTQSLSQRQVQTMRLSPQQLLYTKLLQLPLIQLEQRVKQELEENPMLEEFSESDEEAGQPEEMATDLSQNNDDENKLNE
KSEQNEEDLYIAEKPTKDEPLPKDDDYTKEFDQEIEEYFPDGDEEGFNVPSFREEPSDESYFQPVFHESFTERVLNDLRY
QDVTEKELAIAEEIVGNIDEDGYLRCPIEVIADGLMTTGIEVTEAEILKVLEKIWYIDPVGIGARNLQECLLIQLEAKYQ
RTEDIDDTLYEQAKLILNKYYHEFTMKHFEKLMTLLSISPEELTQALLLIQSFNPKPGGDVSASSNYIIPDFIVTFDGKN
LVAFSNERNSFQIRISEQYKPLLEDKSQPKGAKEFVRNKYEAAKNFMTAIEMRRQTLQRVMNALLNLQYEFFTIGPNRLK
PMILKDVAEVAGVDISTVSRVVNGKYVQAHKGILELKYFFSTGLETESGDEISNKLIKEKIKSFIEKEDTRKPLSDDKIS
EMLQQNGFKVARRTVAKYREQMNIPVARLRKQIQSLK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=515, Percent_Identity=32.4271844660194, Blast_Score=245, Evalue=5e-66,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 59895; Mature: 59895

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQSLSQRQVQTMRLSPQQLLYTKLLQLPLIQLEQRVKQELEENPMLEEFSESDEEAGQ
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCC
PEEMATDLSQNNDDENKLNEKSEQNEEDLYIAEKPTKDEPLPKDDDYTKEFDQEIEEYFP
HHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
DGDEEGFNVPSFREEPSDESYFQPVFHESFTERVLNDLRYQDVTEKELAIAEEIVGNIDE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
DGYLRCPIEVIADGLMTTGIEVTEAEILKVLEKIWYIDPVGIGARNLQECLLIQLEAKYQ
CCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RTEDIDDTLYEQAKLILNKYYHEFTMKHFEKLMTLLSISPEELTQALLLIQSFNPKPGGD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
VSASSNYIIPDFIVTFDGKNLVAFSNERNSFQIRISEQYKPLLEDKSQPKGAKEFVRNKY
CCCCCCEECCCEEEEECCCEEEEECCCCCCEEEEECHHHCCHHCCCCCCCCHHHHHHHHH
EAAKNFMTAIEMRRQTLQRVMNALLNLQYEFFTIGPNRLKPMILKDVAEVAGVDISTVSR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHH
VVNGKYVQAHKGILELKYFFSTGLETESGDEISNKLIKEKIKSFIEKEDTRKPLSDDKIS
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EMLQQNGFKVARRTVAKYREQMNIPVARLRKQIQSLK
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNTQSLSQRQVQTMRLSPQQLLYTKLLQLPLIQLEQRVKQELEENPMLEEFSESDEEAGQ
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCC
PEEMATDLSQNNDDENKLNEKSEQNEEDLYIAEKPTKDEPLPKDDDYTKEFDQEIEEYFP
HHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
DGDEEGFNVPSFREEPSDESYFQPVFHESFTERVLNDLRYQDVTEKELAIAEEIVGNIDE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
DGYLRCPIEVIADGLMTTGIEVTEAEILKVLEKIWYIDPVGIGARNLQECLLIQLEAKYQ
CCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RTEDIDDTLYEQAKLILNKYYHEFTMKHFEKLMTLLSISPEELTQALLLIQSFNPKPGGD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
VSASSNYIIPDFIVTFDGKNLVAFSNERNSFQIRISEQYKPLLEDKSQPKGAKEFVRNKY
CCCCCCEECCCEEEEECCCEEEEECCCCCCEEEEECHHHCCHHCCCCCCCCHHHHHHHHH
EAAKNFMTAIEMRRQTLQRVMNALLNLQYEFFTIGPNRLKPMILKDVAEVAGVDISTVSR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHH
VVNGKYVQAHKGILELKYFFSTGLETESGDEISNKLIKEKIKSFIEKEDTRKPLSDDKIS
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EMLQQNGFKVARRTVAKYREQMNIPVARLRKQIQSLK
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]